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Offlineaomoriakuma
Sensei


Registered: 09/09/13
Posts: 176
Last seen: 7 years, 3 months
no recovery after shaking ):
    #19167477 - 11/20/13 08:47 PM (10 years, 2 months ago)

why, oh why!

i shook 5 jars 5 days ago, and my experience up until now, has been a solid recovery of mycelium starting 2 days after shaking. this time, all 5 jars are doing absolutely nothing! One jar that i shook a few days before those ones(came from the same batch), is at 95% colonization and counting. but the 5 most recent ones just arent doing a damn thing..

is this contamination?

im just so confused.. i did these jars with the best sterile technique i have used of any of my batches. the mycelium started growing quicker than any of my previous batches, and everything just seemed to be running so much smoother than before. then when i do the shake.. everything stalls ):

i DID notice a slight bacterial contam, or what might have been a bacterial contam, in one of the jars. but it was so slight, that i just sort of shrugged it off. ive had worse, and recovered, so i thought nothing of it. but maybe thats the cause? i dont know.. just looking for some opinions.


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Offlinenn-IlliniSpiralDMT
Maniac


Registered: 07/19/12
Posts: 380
Last seen: 7 years, 3 months
Re: no recovery after shaking ): [Re: aomoriakuma]
    #19167520 - 11/20/13 08:55 PM (10 years, 2 months ago)

Probably some contaminates, bacteria or trich are your best bet. Check if the jars turn a different color within a few days.

How did you inoculate? Agar or MS?

What did you do differently than last time, that is the biggest question to ask.


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OfflinecronicrFacebook
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Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
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Trusted Cultivator
Re: no recovery after shaking ): [Re: aomoriakuma]
    #19167548 - 11/20/13 09:03 PM (10 years, 2 months ago)

Quote:

aomoriakuma said:
why, oh why!


i DID notice a slight bacterial contam, or what might have been a bacterial contam, in one of the jars. but it was so slight, that i just sort of shrugged it off. ive had worse, and recovered, so i thought nothing of it.



...................


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Invisiblewildernessjunkie
Reshitivest
I'm a teapot


Registered: 06/13/10
Posts: 8,118
Loc: HTTP 404 Not Found
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Re: no recovery after shaking ): [Re: cronicr]
    #19167617 - 11/20/13 09:16 PM (10 years, 2 months ago)

Bacteria is some agressive shit.


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OfflineMush4Brains
LOOL HACKED!!!

Registered: 07/31/13
Posts: 4,419
Last seen: 9 years, 2 months
Re: no recovery after shaking ): [Re: wildernessjunkie]
    #19167621 - 11/20/13 09:17 PM (10 years, 2 months ago)

Quote:

why, oh why!




Contams, as others have said.


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Offlineaomoriakuma
Sensei


Registered: 09/09/13
Posts: 176
Last seen: 7 years, 3 months
Re: no recovery after shaking ): [Re: Mush4Brains]
    #19167742 - 11/20/13 09:45 PM (10 years, 2 months ago)

i used MS. and the only things i did differently were that i steam dried the WBS, and used better lids that i bought online. overall, the mycelium seemed much healthier than previous jars, and grew exponentially quicker. although i did notice what seemed to be bacterial contamination, it was only in one jar, and covered a total area of about 2 seeds.

i can understand if that one jar was stalled.. but all 5? ):

i hope whatever contamination i have will show itself soon so i can know for sure whats going on!


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OfflineSgt. Pepper
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Registered: 06/19/13
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Last seen: 1 month, 23 days
Re: no recovery after shaking ): [Re: aomoriakuma]
    #19167810 - 11/20/13 10:04 PM (10 years, 2 months ago)

What does it smell like?


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OfflineTheApprentice
back at it
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Registered: 09/25/11
Posts: 4,727
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Re: no recovery after shaking ): [Re: Sgt. Pepper]
    #19168277 - 11/21/13 12:40 AM (10 years, 2 months ago)

If it doesn't recover, it is a contam.

what did u do differently in your sterile work?

it might have been from the master jar, but u said
you had another jar from the same "batch"?

does that mean the contamed jar and the 95%
jar are from the same master?!!


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Offlineaomoriakuma
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Registered: 09/09/13
Posts: 176
Last seen: 7 years, 3 months
Re: no recovery after shaking ): [Re: TheApprentice]
    #19169168 - 11/21/13 09:42 AM (10 years, 2 months ago)

well thats the confusing part, is that i essentially did NOTHING different aside from what i stated before. also, it was an MS syringe.

now.. i DO have another weird theory, and please dont bash me if its completely ridiculous lol. Is it possible that there is water, or a seed, stuck in my SFD, causing there to be a blockage in GE?

oh, and i havent smelled the jar.. but is it even safe to open a colonizing jar without using a flow hood? i dont want to expose it to open air and introduce contams if i dont have to.


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OfflineSgt. Pepper
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Registered: 06/19/13
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Re: no recovery after shaking ): [Re: aomoriakuma]
    #19169804 - 11/21/13 12:22 PM (10 years, 2 months ago)

Quote:

oh, and i havent smelled the jar.. but is it even safe to open a colonizing jar without using a flow hood? i dont want to expose it to open air and introduce contams if i dont have to.



Just smell through the polyfil or tyvek. If it's contaminated with bacteria it will smell sweet in a sickly way, mold will smell stagnant and stale. It's very distinct and you should be able to tell without opening it.


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Offlineaomoriakuma
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Registered: 09/09/13
Posts: 176
Last seen: 7 years, 3 months
Re: no recovery after shaking ): [Re: Sgt. Pepper]
    #19170263 - 11/21/13 02:05 PM (10 years, 2 months ago)

http://out-grow.com/cultivation-equipment-c-1/liquid-culture-or-spawn-jar-lids-p-89.html

im using these lids. cant seem to smell much of anything through it lol. and those are the filters in talking about that i feel MIGHT have gotten clogged and therefore could be restricting GE.. is this possible?


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OfflineSgt. Pepper
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Registered: 06/19/13
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Re: no recovery after shaking ): [Re: aomoriakuma]
    #19170564 - 11/21/13 03:15 PM (10 years, 2 months ago)

Hmm, I suppose it's possible but I don't have any experience with them so I don't really know. Sorry I can't help more.


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InvisibleEnigma1
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Registered: 08/15/13
Posts: 977
Re: no recovery after shaking ): [Re: Sgt. Pepper]
    #19170591 - 11/21/13 03:19 PM (10 years, 2 months ago)

Mine did the same thing I shot 1cc water in it one day and 2cc the next day and it took off.


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InvisibleStygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
Trusted Cultivator
Re: no recovery after shaking ): [Re: Enigma1]
    #19170667 - 11/21/13 03:31 PM (10 years, 2 months ago)

To repeat what's already been said.
Yes, contam.
Shaking is a good contam test, no recovering, no good.


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Invisibledodson

Registered: 10/30/13
Posts: 60
Loc: America Flag
Re: no recovery after shaking ): [Re: StygianKnight]
    #19532405 - 02/06/14 07:44 PM (9 years, 11 months ago)

Quote:

StygianKnight said:
To repeat what's already been said.
Yes, contam.
Shaking is a good contam test, no recovering, no good.




What should I consider  a fair "recovery time"  before I assume contamination or failure?


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OfflineCMOS
Whats next?


Registered: 01/08/12
Posts: 833
Last seen: 9 years, 8 months
Re: no recovery after shaking ): [Re: dodson]
    #19532437 - 02/06/14 07:50 PM (9 years, 11 months ago)

I toss mine if they don't recover in two days.


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InvisibleStygianKnight
A Mushroom

Registered: 03/12/12
Posts: 2,717
Trusted Cultivator
Re: no recovery after shaking ): [Re: CMOS]
    #19532636 - 02/06/14 08:33 PM (9 years, 11 months ago)

Yep!
2-3 days should be showing obvious signs of growth.


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Offlinewolf8312
Pennywise
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Registered: 10/01/12
Posts: 2,356
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Re: no recovery after shaking ): [Re: aomoriakuma]
    #19533659 - 02/07/14 12:53 AM (9 years, 11 months ago)

Quote:

aomoriakuma said:
http://out-grow.com/cultivation-equipment-c-1/liquid-culture-or-spawn-jar-lids-p-89.html

im using these lids. cant seem to smell much of anything through it lol. and those are the filters in talking about that i feel MIGHT have gotten clogged and therefore could be restricting GE.. is this possible?




Well actually, you are using syringe filters instead of SFD's which could well be the problem as everyone says you cannot use these syringe filters for grain jars because they will stall out through lack of proper GE.

I actually started a thread a while back asking how come this vendor was selling these lids when everyone says SF wont work! So maybe you are my long awaited answer and confirmation OP!

I would myself never use any other type of filter material but synthetic filter discs!

Edit- just realized this thread is old!


--------------------
"I'm every nightmare you ever had. I am your worst dreams come true. I am everything you ever were afraid of."

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Edited by wolf8312 (02/07/14 12:55 AM)


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Offlineaomoriakuma
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Registered: 09/09/13
Posts: 176
Last seen: 7 years, 3 months
Re: no recovery after shaking ): [Re: wolf8312]
    #22363005 - 10/11/15 12:09 PM (8 years, 3 months ago)

this thread is super old and i havent been on the forums in a long time lol. BUT, ive been growing consistently since then with literally NO issues EVER.. and i thought back to this recently.

i just wanted to go ahead and state that it WAS NOT contamination. my sterile technique is on point :p it was the lids.. the lids i bought were not meant to be used the way i was hoping to use them. oh well. you live and you learn.

anywho.. new thread for new grows on the way. hope u guys enjoy :p


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OfflineGreenHornThumb
Whisperer Of The Myc
Male


Registered: 10/08/15
Posts: 367
Last seen: 6 months, 16 days
Re: no recovery after shaking ): [Re: Enigma1]
    #22363038 - 10/11/15 12:16 PM (8 years, 3 months ago)

Quote:

Enigma1 said:
Mine did the same thing I shot 1cc water in it one day and 2cc the next day and it took off.




sorry for the added bump but is this correct practice? If i see a stall, I can add a bit of water?  I just shook mine at around 30% and the next day the white starting coming back  up again so no issues. But just wanted to know if this is something that someone can do?


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