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Immortal Jellyfish



Registered: 03/23/13
Posts: 192
Loc: Colorado
Last seen: 22 days, 3 hours
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Have to live in a van for a while. 1
#19163123 - 11/20/13 12:45 AM (10 years, 3 months ago) |
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Hey guys...Long story short, in a few months, I have to sell the house and I am moving to North Dakota to work. I figure with the decent money I'll be making, I will only have to live in the van for a few months. Housing is awful up there.
I have 15 strains that are in Cultube master culture slants. I have to take my slants with me. I bought a small coca-cola personal fridge that works pretty well. It holds about 6 regular sized soda cans and keeps them at 34 degrees F. I will have an electric hook up at night for the culture tubes, but during the day I'll need to drive the van to work and park it.
Will my tubes be alright going from cold to warm on a daily basis like that?
If not, what would you suggest I do to keep my mycelium viable?
Maybe place them in a small thermos that fits in the mini-fridge?
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JMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 10 months
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Heres a post that might help?
Quote:
JMcDoogle said: I posted this as a thread in the advanced section and in two days nobody has responded, maybe I'll get some help here -
Quote:
http://www.fpl.fs.fed.us/documnts/pdf1994/burds94a.pdf
This is the reference article -
Preserving Cultures Using Sterile Distiled Water in Cryovials
Abstract:
Prior to 1985, cultures at teh center for forest mycology research were maintained on a 1.5% malt extract agar test-tube slants. This system not only made it neccessary to transfer the entire collection every year but also permitted genetic change because continual growth occured. In 1985, the method of storing fungal cultures in sterile distilled water in CRYOVIALS was introduced. This study reports on the use of this method for long-term fungal storage. For varying periods of up to seven ( 7 ) years, 151 miscellaneous specials of wood-decaying Basidimycotina were stored in sterile distilled water. Water storage has numerous advantages: culture viability or growth rate is not significantly influenced; isolates can be stored longer; genetic stability is greater; the method is quick, easy, and inexpensive, and requires less space.
Cyrovials ( Internally Threaded & Self Standing
These can withstand autoclaving so if I were to buy them and try something along the lines of distilled water long term storage, I could always uses them as slighty more expensive alternative to the glass vacutainer blood collection tubes.
Heres what Im guessing - seven years, of unrefigerated storage is a possiblity, but even two years unrefigerated would be nice aswell.
Full Sterile - with option of threaded inside, or external - which would you prefer - internal or external threads?
Take a fully colonized grain, or spore print - isolate, isolate, gather your monoculture - inside of SAB/GB/FLOW/FKN LABRATORY
Take 4-6-8 ML of Sterile Distilled Water and put onto your agar monoculture
Scrape it a bit, mix up the myc. Suck it back up and put into the Cyrovial -
Than go about your business and save for 5 years and when needed re-enter onto agar - break down any contams if any - and sustain your strain? Or take fully colonized agar chunk, cut into 2x2 pieces and drop three or four into the cyrovial filled with sterile distilled water, seal and store. They feature a rubber type gasket internally and can be twisted completley air-tight aswell, so no need to wrap them.
Im new to all of this, so this all might be a huge waste of brainpower. Please leave any and all feedback, discreation unneccesary, so... what you think? 
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The ego is nothing other than the focus of conscious attention.
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Immortal Jellyfish



Registered: 03/23/13
Posts: 192
Loc: Colorado
Last seen: 22 days, 3 hours
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Re: Have to live in a van for a while. [Re: JMcDoogle]
#19163159 - 11/20/13 12:56 AM (10 years, 3 months ago) |
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Awesome! Thank you very much JMcDoogle! 5 shrooms for you!
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JMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 10 months
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The ego is nothing other than the focus of conscious attention.
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fancy
Stranger


Registered: 11/16/13
Posts: 5
Last seen: 10 years, 8 days
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Re: Have to live in a van for a while. [Re: JMcDoogle]
#19163598 - 11/20/13 05:04 AM (10 years, 3 months ago) |
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It's gonna be reeeaally cold here in a few months! Like way sub-zero. Housing isn't so hard to find in Minot. Williston area is pretty fucked though. I know that doesn't really address your question or anything but it's neat to see someone else coming to work in the middle of nowhere like I did. It's actually not as bad as I thought it was gonna be.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 12 hours, 41 minutes
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Re: Have to live in a van for a while. [Re: fancy]
#19163645 - 11/20/13 05:57 AM (10 years, 3 months ago) |
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Living in a van down by the river
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Re: Have to live in a van for a while. [Re: Stromrider]
#19163768 - 11/20/13 06:49 AM (10 years, 3 months ago) |
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Quote:
Stromrider said: Living in a van down by the river 
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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SupaThaRipper
Genetics Hoarder



Registered: 09/02/13
Posts: 1,688
Loc: USA
Last seen: 6 minutes, 12 seconds
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Re: Have to live in a van for a while. [Re: PussyFart]
#19163789 - 11/20/13 06:59 AM (10 years, 3 months ago) |
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Kind of off your question but do you want to be driving around everyday for months in your van with that stuff in there? Im just wondering what the charges would be. I too have strongly thought about going to ND but I dont want to lose my mushies
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kratom_redmomd
Kratom Lover



Registered: 07/25/13
Posts: 411
Loc: Hiking Cascadia
Last seen: 4 years, 4 months
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Re: Have to live in a van for a while. [Re: SupaThaRipper]
#19164100 - 11/20/13 09:20 AM (10 years, 3 months ago) |
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Driving around with them would not be.my first choice. Do you have a friend that could store them for you and then FedEx them to you once you were settled? Just a thought.
-------------------- "If the goal is to ride the bike, why is everyone arguing about which bike to ride? We are all bike riders. Ride your bike." -Unknown If I was going to destroy a nation, first I would destroy its language. -Confusious ¿Hablas Inglés?
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JMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 10 months
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Depending on the state its probably not a big deal, if he WERE busted, it would probably weigh less than a gram of illegal material. misdemeanor.
Although... the fuck you wanna be drivin around in a van with a bunch of culture slants for, they gon' find you.
lol I could go either way on this...
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The ego is nothing other than the focus of conscious attention.
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Sgt. Pepper



Registered: 06/19/13
Posts: 2,538
Loc: Third Stone From The Sun
Last seen: 2 months, 17 days
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Re: Have to live in a van for a while. [Re: JMcDoogle]
#19164305 - 11/20/13 10:14 AM (10 years, 3 months ago) |
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Quote:
JMcDoogle said: Depending on the state its probably not a big deal, if he WERE busted, it would probably weigh less than a gram of illegal material. misdemeanor.
Although... the fuck you wanna be drivin around in a van with a bunch of culture slants for, they gon' find you.
lol I could go either way on this...
I'm pretty sure mycelial growth and mushrooms at any stage is a felony. Mycelium contains psilocybin so that puts it at felony level.
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JMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 10 months
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Re: Have to live in a van for a while. [Re: Sgt. Pepper]
#19164318 - 11/20/13 10:17 AM (10 years, 3 months ago) |
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Quote:
Sgt. Pepper said:
Quote:
JMcDoogle said: Depending on the state its probably not a big deal, if he WERE busted, it would probably weigh less than a gram of illegal material. misdemeanor.
Although... the fuck you wanna be drivin around in a van with a bunch of culture slants for, they gon' find you.
lol I could go either way on this...
I'm pretty sure mycelial growth and mushrooms at any stage is a felony. Mycelium contains psilocybin so that puts it at felony level.
Damn, I guess every kid who gets popped with an eighter is a felon these days...
Not likely, its probably based on weight. Felony possesion > Possesion
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The ego is nothing other than the focus of conscious attention.
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TiN 42
Be the tree



Registered: 12/15/11
Posts: 1,242
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Re: Have to live in a van for a while. [Re: JMcDoogle]
#19164409 - 11/20/13 10:36 AM (10 years, 3 months ago) |
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I've always been told that mycel & cultures where not illegal unless there is mushrooms growing in the same space. I would also be more worried about them freezing.
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MUSH LOVE
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Have to live in a van for a while. [Re: TiN 42]
#19164477 - 11/20/13 10:50 AM (10 years, 3 months ago) |
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I don't think the cops would know what the culture tubes were. You could probably just say there button mushrooms or beer yeast and never have another question asked.
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ForgottenFreshness
Staying High


Registered: 11/16/13
Posts: 211
Last seen: 4 months, 25 days
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Re: Have to live in a van for a while. [Re: bodhisatta]
#19164559 - 11/20/13 11:05 AM (10 years, 3 months ago) |
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Why would he be getting busted? I mean if these cultures are in his van inside of a mini fridge. It's not like they are laying out in the open as long as the guy isn't smoking tons of blunts in the van while driving around town there's no reason po would have to investigate.
You people are WWWWAAAAAYYYYY to paranoid.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
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Quote:
ForgottenFreshness said: Why would he be getting busted? I mean if these cultures are in his van inside of a mini fridge. It's not like they are laying out in the open as long as the guy isn't smoking tons of blunts in the van while driving around town there's no reason po would have to investigate.
You people are WWWWAAAAAYYYYY to paranoid.
Unless you live in Chicago, where they search you and your car for traffic violations, with no probable cause, 90% of the time.
Even walking down the street you can be stopped and searched, while being white on a sunday.....it's fucking nuts.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Quote:
ForgottenFreshness said: Why would he be getting busted? I mean if these cultures are in his van inside of a mini fridge. It's not like they are laying out in the open as long as the guy isn't smoking tons of blunts in the van while driving around town there's no reason po would have to investigate.
You people are WWWWAAAAAYYYYY to paranoid.
if i were a cop and seen a ffridge in a van i'd look
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: Have to live in a van for a while. [Re: PussyFart]
#19164607 - 11/20/13 11:13 AM (10 years, 3 months ago) |
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Quote:
Notahacker420 said:
Quote:
ForgottenFreshness said: Why would he be getting busted? I mean if these cultures are in his van inside of a mini fridge. It's not like they are laying out in the open as long as the guy isn't smoking tons of blunts in the van while driving around town there's no reason po would have to investigate.
You people are WWWWAAAAAYYYYY to paranoid.
Unless you live in Chicago, where they search you and your car for traffic violations, with no probable cause, 90% of the time.
Even walking down the street you can be stopped and searched, while being white on a sunday.....it's fucking nuts.
It's against the law to not have road hazard kits, tire iron etc... so they have to "look for those" extra ticket if you dont have them $$ if they find something else *bonus*
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ForgottenFreshness
Staying High


Registered: 11/16/13
Posts: 211
Last seen: 4 months, 25 days
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Re: Have to live in a van for a while. [Re: cronicr]
#19164738 - 11/20/13 11:35 AM (10 years, 3 months ago) |
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Man, I'm glad I don't live even remotely close to any major city. Sounds fucked up
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Quote:
ForgottenFreshness said: Man, I'm glad I don't live even remotely close to any major city. Sounds fucked up
Chicago is one of the worst. Irony is that the cops and politics are part of the reason causing the outrageous crime rates. Poor poor people living in poverty with low education voting for the people that are hurting them the most. It's your city, why are you letting the cops get this crazy. Oh yea those alderpeople, mayors, governors, judges, police chiefs etc that you elected.
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