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using living mushroom to start mycelium growth in honey
    #1916249 -

I want to use a live mushroom to start some mycelium in honey.
I need to know:
- howmuch honey
- water
- howmuch mushroom
- Should I pressure cook or not?

I will be using 1 pint widemouth jars and I have tons of micron magic filter disks. I have a glovebox as well with a fan for positive pressure.


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http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1916258 -

125ml's of water 1/4ts of honey then pc and just a small piece of mushroom

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Re: using living mushroom to start mycelium growth in honey [Re: buu]
    #1917942 -

how long should I pc it for?


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http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1917996 -

1/4 honey is way too much. its more like 4%. 5-10 mls of honey shoulkd be plenty for 125ml water. then PC for 15 ms :smile: 


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comario


"crusaders against emotional poverty"

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Re: using living mushroom to start mycelium growth in honey [Re: comario2]
    #1918026 -

I assumed they meant 1/4 teaspoon, which would work fine.. about the same as what I've just used to clone some mushies..

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Re: using living mushroom to start mycelium growth in honey [Re: Sorted]
    #1918137 -

Hmmm, I donno about the 125 of water, what I have is 1 pint wide mouth jars, I just need to know howmuch water should be in them and howmuch honey.


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http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1918178 -

How about putting the 125ml of water and 1/4 teaspoon of honey into a 1 pint jar? I'm fairly sure it'll fit :tongue:

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1918257 -

Since you will be using live tissue and not spores, you could add some peroxide to make it almost foolproof.

About 3cc of hydrogen peroxide solution should be about right for a pint, assuming your peroxide is a 3% solution like most. Check the bottle.


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https://www.gofundme.com/f/support-for-renee-goods-wife-and-son

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Re: using living mushroom to start mycelium growth in honey [Re: Baby_Hitler]
    #1918283 -

sooo...if im hearing all this correct....u could take some honey...some water.....a chunk of fresh shroom..............and grow mycelium????

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Re: using living mushroom to start mycelium growth in honey [Re: ]
    #1918518 -

bingo :laugh: 


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comario


"crusaders against emotional poverty"

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Re: using living mushroom to start mycelium growth in honey [Re: comario2]
    #1918911 -

Yeah, and then transfer the mycelium into 1 pint jars full of substrate, that way I don't have to wait 1-2 weeks for the spores to start colonizing.


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http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1919844 -


I guess you would have to add H202 *after* pressure cooking the jar. I think H202 will break down and become useless if pressure cooked. Am I wrong?

.fs.

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Re: using living mushroom to start mycelium growth in honey [Re: fIsh in my head]
    #1920087 -

Well I don't really see why I need h202 if I pressure cook. All I would need to do is put a micron disk over the top, close the jar, and then pressure cook. I don't see a need for h202 except when I will use a syringe to extract mycelium and transfer it to other jars.


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http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1920271 -


Yeah, you don't need H202 to sterilize your jar. But I think hitler is worried about the little piece of shroom you will add to your jar that might not be sterile!

That's why H202 will help.

At least, that's how I figure it.

.fs.

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1920274 -

dude you dont even need to use the filter disc. it doesnt need that much air exchange. just use a regular lid.


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Farewell FRSE!  We had such good times together.

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Re: using living mushroom to start mycelium growth in honey [Re: acidhead1279]
    #1920547 -

hmm i gotta start doing this, but then I would have to build a glove box to do the mycellum to substrate transfers.. fuck.

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Re: using living mushroom to start mycelium growth in honey [Re: whole9]
    #1920561 -

whole9.....this glovebox will take you less than a half hour to make.
www.geocities.com/sheet_02


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Coaster is an idiot...
[quote]Coaster said:
but i thnk everything thats pure is white?
[/quote]



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Re: using living mushroom to start mycelium growth in honey [Re: Hanky]
    #1922324 -

Well thanx people, Im gonna get a mushroom from my bud and clone the sucka. Gracias


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http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"

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a [Re: downforpot]
    #1922566 -

a

Edited by enacid (01/30/08 01:17 AM)

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Re: using living mushroom to start mycelium growth in honey [Re: enacid]
    #1922595 -

um.. one.. ml and cc are the same thing

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Re: using living mushroom to start mycelium growth in honey [Re: enacid]
    #1922878 -

WTF!!!!

how come everybody is on my ignore list???


--------------------
Welcome to my world!


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Re: using living mushroom to start mycelium growth in honey [Re: enacid]
    #1922937 -

1ml=1cc  :crazy2:


--------------------
Don't fuck with me
I'm a postal worker;-)
A zen master once said to me
don't do anything I tell you to do
so i did

<p><a href="http://www.whump.com/dropbox/other/ujname.html">Get yours</a>.</p>

<p>My <a href="http://tinyurl.com/6valr">Unitarian Jihad Name</a> is: <strong>Brother Neutron Bomb of Compassion</strong>.</p>
<p><a href="http://www.whump.com/dropbox/other/ujname.html">Get yours</a>.</p>

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Re: using living mushroom to start mycelium growth in honey [Re: enacid]
    #1922954 -

1ml = 1cc

isn't the metric system smart...



--------------------
Hop in my Chrysler;
it's as big as a Whale, and it's about to set Sail.

Oh yeah and if anyone knows of any job openings for a Mechanical Engineer in the Boulder area let me know. tks

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a [Re: Pawn_Star]
    #1923109 -

a

Edited by enacid (01/30/08 01:17 AM)

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Re: using living mushroom to start mycelium growth in honey [Re: comario2]
    #1951476 -

I just bought some organic honey and the stuff is solid, I used a spoon to taste some of it,will this stuff work?


--------------------



http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1951485 -

Yeah it will.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Re: using living mushroom to start mycelium growth in honey [Re: SixCee]
    #1951489 -

How do I know how much I need? I got my graduated cylinder ready. I'll need 5-10mls of honey, howmuch should I dissolve in howmuch water?


--------------------



http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"

Edited by downforpot (09/25/03 03:17 PM)

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1951532 -

http://www.shroomery.org/index/par/8005 You can easily improvise with this if you like.


--------------------
~~~~~~~~~~~~~~~~~~~~~~~~
-->The above statements may or not be true.
->Quote of the Moment :
"Yea. All bitches are whores who love sex." -Cubie
----> PMs checked daily.

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Re: using living mushroom to start mycelium growth in honey [Re: SixCee]
    #1951537 -

My friend used WAY less honey than was suggested and his jar appeares to be working.


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Fiddlesticks.


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Re: using living mushroom to start mycelium growth in honey [Re: Rose]
    #1951545 -

Sweet, thanks for the link SixCee, I got the same honey as in the tek.


--------------------



http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
Can't fuck with me again"

Edited by downforpot (09/25/03 03:29 PM)

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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1951750 -

dfp I'd soak the piece of shroom your planning to use in h202/h20 solution before adding it....I've done this for g2g transfers and had almost 100% success without the use of a glovebox

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Re: using living mushroom to start mycelium growth in honey [Re: ]
    #1951793 -

My friend, Don Quixote, appears to be growing mycleum from a dry specimen that he soaked in H2O2.

http://www.shroomery.org/forums/showflat...;o=&fpart=1

Everything he, and I, have read lead us to believe this can not happen but... the proof is in the pudding... er... honey water.

I highly recomend using H202 from the begining of the process when you are using living mushies in honeywater.


--------------------
Fiddlesticks.


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