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Invisibledownforpot
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using living mushroom to start mycelium growth in honey
    #1916249 - 09/14/03 08:24 PM (13 years, 2 months ago)

I want to use a live mushroom to start some mycelium in honey.
I need to know:
- howmuch honey
- water
- howmuch mushroom
- Should I pressure cook or not?

I will be using 1 pint widemouth jars and I have tons of micron magic filter disks. I have a glovebox as well with a fan for positive pressure.


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Invisiblebuu
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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1916258 - 09/14/03 08:28 PM (13 years, 2 months ago)

125ml's of water 1/4ts of honey then pc and just a small piece of mushroom


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Invisibledownforpot
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Re: using living mushroom to start mycelium growth in honey [Re: buu]
    #1917942 - 09/15/03 01:31 PM (13 years, 2 months ago)

how long should I pc it for?


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Offlinecomario2
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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1917996 - 09/15/03 01:54 PM (13 years, 2 months ago)

1/4 honey is way too much. its more like 4%. 5-10 mls of honey shoulkd be plenty for 125ml water. then PC for 15 ms :smile: 


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InvisibleSorted
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Re: using living mushroom to start mycelium growth in honey [Re: comario2]
    #1918026 - 09/15/03 02:14 PM (13 years, 2 months ago)

I assumed they meant 1/4 teaspoon, which would work fine.. about the same as what I've just used to clone some mushies..


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Invisibledownforpot
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Re: using living mushroom to start mycelium growth in honey [Re: Sorted]
    #1918137 - 09/15/03 02:49 PM (13 years, 2 months ago)

Hmmm, I donno about the 125 of water, what I have is 1 pint wide mouth jars, I just need to know howmuch water should be in them and howmuch honey.


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InvisibleSorted
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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1918178 - 09/15/03 03:01 PM (13 years, 2 months ago)

How about putting the 125ml of water and 1/4 teaspoon of honey into a 1 pint jar? I'm fairly sure it'll fit :tongue:


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OfflineBaby_Hitler
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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1918257 - 09/15/03 03:30 PM (13 years, 2 months ago)

Since you will be using live tissue and not spores, you could add some peroxide to make it almost foolproof.

About 3cc of hydrogen peroxide solution should be about right for a pint, assuming your peroxide is a 3% solution like most. Check the bottle.


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Anonymous

Re: using living mushroom to start mycelium growth in honey [Re: Baby_Hitler]
    #1918283 - 09/15/03 03:37 PM (13 years, 2 months ago)

sooo...if im hearing all this correct....u could take some honey...some water.....a chunk of fresh shroom..............and grow mycelium????


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Offlinecomario2
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Re: using living mushroom to start mycelium growth in honey [Re: ]
    #1918518 - 09/15/03 04:45 PM (13 years, 2 months ago)

bingo :laugh: 


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Invisibledownforpot
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Re: using living mushroom to start mycelium growth in honey [Re: comario2]
    #1918911 - 09/15/03 07:05 PM (13 years, 2 months ago)

Yeah, and then transfer the mycelium into 1 pint jars full of substrate, that way I don't have to wait 1-2 weeks for the spores to start colonizing.


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OfflinefIsh in my head
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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1919844 - 09/15/03 11:33 PM (13 years, 2 months ago)


I guess you would have to add H202 *after* pressure cooking the jar. I think H202 will break down and become useless if pressure cooked. Am I wrong?

.fs.


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Invisibledownforpot
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Re: using living mushroom to start mycelium growth in honey [Re: fIsh in my head]
    #1920087 - 09/16/03 12:49 AM (13 years, 2 months ago)

Well I don't really see why I need h202 if I pressure cook. All I would need to do is put a micron disk over the top, close the jar, and then pressure cook. I don't see a need for h202 except when I will use a syringe to extract mycelium and transfer it to other jars.


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http://www.myspace.com/4th25


"And I don't care if he was handcuffed
Then shot in his head
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Can't fuck with me again"


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OfflinefIsh in my head
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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1920271 - 09/16/03 02:18 AM (13 years, 2 months ago)


Yeah, you don't need H202 to sterilize your jar. But I think hitler is worried about the little piece of shroom you will add to your jar that might not be sterile!

That's why H202 will help.

At least, that's how I figure it.

.fs.


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Offlineacidhead1279
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Re: using living mushroom to start mycelium growth in honey [Re: downforpot]
    #1920274 - 09/16/03 02:19 AM (13 years, 2 months ago)

dude you dont even need to use the filter disc. it doesnt need that much air exchange. just use a regular lid.


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Offlinewhole9
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Re: using living mushroom to start mycelium growth in honey [Re: acidhead1279]
    #1920547 - 09/16/03 04:33 AM (13 years, 2 months ago)

hmm i gotta start doing this, but then I would have to build a glove box to do the mycellum to substrate transfers.. fuck.


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InvisibleHanky
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Re: using living mushroom to start mycelium growth in honey [Re: whole9]
    #1920561 - 09/16/03 04:44 AM (13 years, 2 months ago)

whole9.....this glovebox will take you less than a half hour to make.
www.geocities.com/sheet_02


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Invisibledownforpot
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Re: using living mushroom to start mycelium growth in honey [Re: Hanky]
    #1922324 - 09/16/03 07:02 PM (13 years, 2 months ago)

Well thanx people, Im gonna get a mushroom from my bud and clone the sucka. Gracias


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"And I don't care if he was handcuffed
Then shot in his head
All I know is dead bodies
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Invisibleenacid
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a [Re: downforpot]
    #1922566 - 09/16/03 08:01 PM (13 years, 2 months ago)

a


Edited by enacid (01/30/08 03:17 AM)


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InvisibleSorted
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Re: using living mushroom to start mycelium growth in honey [Re: enacid]
    #1922595 - 09/16/03 08:09 PM (13 years, 2 months ago)

um.. one.. ml and cc are the same thing


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Amazon Shop for: ½ Pint Jars

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