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OfflineMaeon
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Registered: 11/19/13
Posts: 2
Last seen: 10 years, 2 months
Liquid culture growth span?
    #19160491 - 11/19/13 04:28 PM (10 years, 2 months ago)

I injected a mixture of 4 parts light Karo syrup to 96 parts distilled water with 6cc of a MS syringe.  It has been 10 days and I'm only seeing minimal signs of growth.  I did this with 9 different strains and they are being kept in a locker with slight air movement,  a constant temp of 81 degrees and the have a .5 micron filter disk on each jar.  Is this normal or has something gone wrong?


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
Trusted Cultivator
Re: Liquid culture growth span? [Re: Maeon]
    #19160498 - 11/19/13 04:31 PM (10 years, 2 months ago)

LCs from spores are sketchy...my advice is to forget all about LCs, and focus more on agar and G2G.

That being said, th only way to test an LC is to shoot up a test jar.

There is no way to visually tell if it is good or not....

Only use distilled water for spore syringes....it is a solvent.


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OfflineOregonMushys
Rye Wata Whippin
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Registered: 09/09/13
Posts: 280
Last seen: 6 years, 4 months
Re: Liquid culture growth span? [Re: Maeon]
    #19160788 - 11/19/13 05:21 PM (10 years, 2 months ago)

Quote:

Maeon said:
I injected a mixture of 4 parts light Karo syrup to 96 parts distilled water with 6cc of a MS syringe.  It has been 10 days and I'm only seeing minimal signs of growth.  I did this with 9 different strains and they are being kept in a locker with slight air movement,  a constant temp of 81 degrees and the have a .5 micron filter disk on each jar.  Is this normal or has something gone wrong?



6cc of spore solution can be used to expand 6 colonized jars into like 54, the 54 jars got a potential to expand into 200-480 fully colonized jars in a matter of a couple months, all by means of g2g. If you own that many jars, and considering one has a PC, SAB, tyvek arm sleeves, iso, loads of grains, etc. Those 200-480 jars can each be g2g into their own 10qt spawn bag, which will give you about 2,000-4,800qts of spawn. It depends on how hard you really wanna go and if you wanna buy 400 jars and spawn bags. Im dreamin.
:bongload::lol:

Check This Out


--------------------
Ps. Cubensis                                        Ps. Cyanescens                                      Ps. Stuntzii  *GrowLog*
   
                                                                                                      :sasquatch::mushroomgrow::leaf::mushroomgrow::leaf::mushroomgrow:
                                                                                                  :cop:


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