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Maeon
Stranger
Registered: 11/19/13
Posts: 2
Last seen: 10 years, 2 months
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Liquid culture growth span?
#19160491 - 11/19/13 04:28 PM (10 years, 2 months ago) |
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I injected a mixture of 4 parts light Karo syrup to 96 parts distilled water with 6cc of a MS syringe. It has been 10 days and I'm only seeing minimal signs of growth. I did this with 9 different strains and they are being kept in a locker with slight air movement, a constant temp of 81 degrees and the have a .5 micron filter disk on each jar. Is this normal or has something gone wrong?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 16 hours
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Re: Liquid culture growth span? [Re: Maeon]
#19160498 - 11/19/13 04:31 PM (10 years, 2 months ago) |
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LCs from spores are sketchy...my advice is to forget all about LCs, and focus more on agar and G2G.
That being said, th only way to test an LC is to shoot up a test jar.
There is no way to visually tell if it is good or not....
Only use distilled water for spore syringes....it is a solvent.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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OregonMushys
Rye Wata Whippin



Registered: 09/09/13
Posts: 280
Last seen: 6 years, 4 months
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Re: Liquid culture growth span? [Re: Maeon]
#19160788 - 11/19/13 05:21 PM (10 years, 2 months ago) |
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Quote:
Maeon said: I injected a mixture of 4 parts light Karo syrup to 96 parts distilled water with 6cc of a MS syringe. It has been 10 days and I'm only seeing minimal signs of growth. I did this with 9 different strains and they are being kept in a locker with slight air movement, a constant temp of 81 degrees and the have a .5 micron filter disk on each jar. Is this normal or has something gone wrong?
6cc of spore solution can be used to expand 6 colonized jars into like 54, the 54 jars got a potential to expand into 200-480 fully colonized jars in a matter of a couple months, all by means of g2g. If you own that many jars, and considering one has a PC, SAB, tyvek arm sleeves, iso, loads of grains, etc. Those 200-480 jars can each be g2g into their own 10qt spawn bag, which will give you about 2,000-4,800qts of spawn. It depends on how hard you really wanna go and if you wanna buy 400 jars and spawn bags. Im dreamin.
 
Check This Out
-------------------- Ps. Cubensis Ps. Cyanescens Ps. Stuntzii *GrowLog*
    
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