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Offlineunderfliptown
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Simple question about potato dextrose
    #19154864 - 11/18/13 03:45 PM (10 years, 2 months ago)

How much potato dextrose to water should you use in an LC?

Also, i assume you PC this after you make it. How long should you PC it at 15psi?


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OfflinePussyFart
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19154874 - 11/18/13 03:46 PM (10 years, 2 months ago)

I suggest not even doing an LC...it's just a waste of time IMHO.

Just knock up what you got with spores....there will be less risk, and less wasted time.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: PussyFart]
    #19154896 - 11/18/13 03:49 PM (10 years, 2 months ago)

OK... but I am gonna do an LC. So if you could answer the question....

Sterile procedures arent hard.


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19154908 - 11/18/13 03:51 PM (10 years, 2 months ago)



--------------------
The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19154911 - 11/18/13 03:51 PM (10 years, 2 months ago)

holy shit... you guys should just not post if you're not going to help. Annoying.

I already used the search function.


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OfflinePussyFart
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19154916 - 11/18/13 03:52 PM (10 years, 2 months ago)

Quote:

underfliptown said:
OK... but I am gonna do an LC. So if you could answer the question....

Sterile procedures arent hard.



Sterile procedures have nothing to do with the fact that spore syringes/prints are hardly ever 100% clean.

This is because the cap from which they came was more than likely fruited in open air.

LC is the perfect breeding ground for everything not fungi.

I do not have an answer to your question, as I have never used potato dextrose....

But all I can say is steer away from any LC that was not made from a clean agar wedge.


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19154919 - 11/18/13 03:53 PM (10 years, 2 months ago)

Could you not find this?

or This

or This

I could go all day.

That took twenty seconds.


--------------------
The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: PussyFart]
    #19154924 - 11/18/13 03:54 PM (10 years, 2 months ago)

If you sterilize your syringe it will kill any bacteria.


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19154929 - 11/18/13 03:54 PM (10 years, 2 months ago)

Dudes a troll.


--------------------
The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19154933 - 11/18/13 03:54 PM (10 years, 2 months ago)

Quote:

JMcDoogle said:
Could you not find this?

or This

or This

I could go all day.

That took twenty seconds.




Why do you gotta be such a dick about it man. You're really coming across as an asshole.

In the same breath, thank you for the links.


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OfflinePussyFart
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19154935 - 11/18/13 03:54 PM (10 years, 2 months ago)

Quote:

JMcDoogle said:
Could you not find this?

or This

or This

I could go all day.

That took twenty seconds.



Those links might be useful, if he was trying to do agar......
Quote:

underfliptown said:
If you sterilize your syringe it will kill any bacteria.



You are going to sterilize a syringe with spores inside?

This will also kill the spores.....


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: PussyFart]
    #19154938 - 11/18/13 03:55 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Quote:

JMcDoogle said:
Could you not find this?

or This

or This

I could go all day.

That took twenty seconds.



Those links might be useful, if he was trying to do agar......
Quote:

underfliptown said:
If you sterilize your syringe it will kill any bacteria.



You are going to sterilize a syringe with spores inside?

This will also kill the spores.....




no no I will clean the syringe on the outside with iso


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: PussyFart]
    #19154943 - 11/18/13 03:55 PM (10 years, 2 months ago)

Excuse me NAH but they will work just fine,

Seing as you are not adding the Agar-Agar to the recipe.

You stand corrected.


--------------------
The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19154950 - 11/18/13 03:56 PM (10 years, 2 months ago)

Quote:

JMcDoogle said:
Could you not find this?

or This

or This

I could go all day.

That took twenty seconds.




This is all agar man... Try reading my question. I was looking for info on LC. Just stop posting in my threads if you're just gonna be a jerk.


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19154955 - 11/18/13 03:56 PM (10 years, 2 months ago)

Read my last post. Friend.


--------------------
The ego is nothing other than the focus of conscious attention.


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OfflinePussyFart
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19154957 - 11/18/13 03:57 PM (10 years, 2 months ago)

Yea....trollito.

Alcohol does not sterilize, it sanitizes.

Flame your needle between jars, do not wipe with an unsterile substance.

The fact still remains that no spore syringe or print is ever 100% clean....no matter what your sterile procedures are like.
Quote:

JMcDoogle said:
Excuse me NAH but they will work just fine,

Seing as you are not adding the Agar-Agar to the recipe.

You stand corrected.



Never tried it, but if it works.....it works lol.....


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19154961 - 11/18/13 03:57 PM (10 years, 2 months ago)

Pay attention almighty troll!

PS. I like you. Can we be friends?
:aweohyou:


--------------------
The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19154979 - 11/18/13 03:59 PM (10 years, 2 months ago)

lol ok I made one trolly thread yesterday... Big deal. It actually ended up turning into a good discussion about strains and I learned a lot.

I am new to this and have only ever done pftek. I wanna get it right so i am asking a bunch of questions. I thought that is what this is for. as you can see i was not so successful on my last grow:





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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19154992 - 11/18/13 04:01 PM (10 years, 2 months ago)

Your picture is sideways,

You can open a picture in Windows Picture Viewer and rotate it.

Next time, rotate it.

I dont like tilting my head.

Nobody puts baby in a corner.

:trollhide:


--------------------
The ego is nothing other than the focus of conscious attention.


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OfflinePussyFart
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19154997 - 11/18/13 04:02 PM (10 years, 2 months ago)

:smbfacepalm:


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: PussyFart]
    #19155002 - 11/18/13 04:03 PM (10 years, 2 months ago)

soooooooooooooo....

My question still hasn't been answered...

ANyone?


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: PussyFart]
    #19155029 - 11/18/13 04:06 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
:smbfacepalm:




Reverse trolling, It works!

broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)

I suggest adding a small amount of Karo ( Light Corn Syrup )

10-20 ML


--------------------
The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19155040 - 11/18/13 04:08 PM (10 years, 2 months ago)

Quote:

JMcDoogle said:
Quote:

Notahacker420 said:
:smbfacepalm:




Reverse trolling, It works!

broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)

I suggest adding a small amount of Karo ( Light Corn Syrup )

10-20 ML




See how hard was that?! I just hope  you're serious right now and not setting me up for failure. Cause that would be really uncool!


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19155046 - 11/18/13 04:08 PM (10 years, 2 months ago)

Im not an ass, I wouldn't do that.

Good Luck
:fuckinawesome:


--------------------
The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19155057 - 11/18/13 04:10 PM (10 years, 2 months ago)

Thanks man. I am really excited to get a monotub going! I got r44 and zstrain to work with!


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Offlinetwistedty
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19155070 - 11/18/13 04:11 PM (10 years, 2 months ago)

if you have your hands on pda why make an lc, pour some dishes and save a few weeks of your life.

other than that if you insist on making an LC :derpyum:

there are very very simple recipes all over the forums.

just sayin.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: twistedty]
    #19155081 - 11/18/13 04:12 PM (10 years, 2 months ago)

Quote:

twistedty said:
if you have your hands on pda why make an lc, pour some dishes and save a few weeks of your life.

other than that if you insist on making an LC :derpyum:

there are very very simple recipes all over the forums.

just sayin.




What do you mean pour some dishes?


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Offlinetwistedty
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19155094 - 11/18/13 04:14 PM (10 years, 2 months ago)

i would look for agar recipes instead of LCs, but if your heart is set on LCs good luck


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: twistedty]
    #19155097 - 11/18/13 04:14 PM (10 years, 2 months ago)

No i don't have potato dextrose agar, its just potato dextrose.


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OfflinePussyFart
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19155116 - 11/18/13 04:16 PM (10 years, 2 months ago)

Quote:

underfliptown said:
No i don't have potato dextrose agar, its just potato dextrose.



So get some agar, and some petri dishes....

http://www.mushroomvideos.com/Agar-Petri-Dishes

http://www.mushroomvideos.com/Mushroom-Cloning

http://www.mushroomvideos.com/Strain-Isolation

http://www.mushroomvideos.com/Master-Culture-Slants


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: PussyFart]
    #19155125 - 11/18/13 04:17 PM (10 years, 2 months ago)

Do you have to buy agar online?


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OfflinePussyFart
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19155138 - 11/18/13 04:19 PM (10 years, 2 months ago)

Quote:

underfliptown said:
Do you have to buy agar online?



No....you can find it at asian markets, if there are any on your local area...or at some major grocery chains...

If not, online might just be the easiest way....


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Offlinetwistedty
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19155146 - 11/18/13 04:21 PM (10 years, 2 months ago)

Quote:

underfliptown said:
Do you have to buy agar online?




nope you can get agar agar from health food stores, and mix your pd with it.  you can get away with no pour petris if you dont have the cash for petris.

which online is your best deal

this is what i use

http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=PDAA01


and get a whole bunch of these

http://www.mycosupply.com/cgi-bin/shopper.cgi?preadd=action&key=SFDI01


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Offlinetwistedty
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Re: Simple question about potato dextrose [Re: twistedty]
    #19155150 - 11/18/13 04:22 PM (10 years, 2 months ago)

when i jumped into agar i had a lil cash so i bought a case of 500 petris, and 500g of pda.


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OfflinePsilicon
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19155207 - 11/18/13 04:33 PM (10 years, 2 months ago)

Quote:

underfliptown said:
soooooooooooooo....

My question still hasn't been answered...

ANyone?




For the record, and for anyone else reading this, the difference between a nutrient broth and an agar plate is the agar and the plate.  Anything that will grow on PDA or LMEA will grow in potato dextrose broth or a solution of light malt extract just as easily.  The concentrations of sugars and such are the same.

Earlier, underfliptown, you were talking about sanitizing the outside of your syringe with alcohol.  That's cool and all, but what you should do instead is make a self-healing injection port and a tyvek breathing patch, so that way you're limited to bacteria that are inside the syringe.  With a good vendor and using only a single drop of spore solution you'll have about a 50% success rate, so make several LCs at the same time and do some small test jars.


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InvisiblePastywhyteMDiscord
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Re: Simple question about potato dextrose [Re: twistedty]
    #19155211 - 11/18/13 04:34 PM (10 years, 2 months ago)

Once you have your clean culture on agar, if your heart is still set on LC, you can use an agar wedge to inoculate your LC and maybe have a chance that it will work. However IMO, LC is a complete waste of time if your growing cubensis, as G2G is faster and safer in the long run. Save LC for exotics or edibles. On a side note my p galindoi LC is looking good, should be able to test it on agar or a single test jar soon to see if its even worth using :lol:


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Re: Simple question about potato dextrose [Re: PussyFart]
    #19155276 - 11/18/13 04:44 PM (10 years, 2 months ago)

I am just a beginner, but using agar to isolate genomes and cloning is really easy.  Agar Agar is very inexpensive in grocery stores(in the Asian section) and Asian markets.  I paid only $1.69 USD for this package:



It is easy to work with.  Using an agar media took a little longer at first, but now I have a good sample that I can continue to use while testing different genomes and strains at the same time. Being a beginner myself, I am trying the tried and tested methods first, and I can figure out a way to make things work better for myself after.

Plus, working with agar is so interesting.  You can really get behind the science of the growth. 

I use something similar to a potato dextrose agar but I substitute the starch water with potato flakes and I use Karo for the nutrient.  It works great and it is super cheap. Happy growing :smile:

Edit: For less than $15 USD, I have enough to make 4 liters  of agar media.  This is about enough for 120 petri dishes.  Do it man.  You won't regret it.


Edited by Cheezymold (11/18/13 04:48 PM)


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: Psilicon]
    #19155278 - 11/18/13 04:44 PM (10 years, 2 months ago)

Quote:

van der griegen said:
Quote:

underfliptown said:
soooooooooooooo....

My question still hasn't been answered...

ANyone?




For the record, and for anyone else reading this, the difference between a nutrient broth and an agar plate is the agar and the plate.  Anything that will grow on PDA or LMEA will grow in potato dextrose broth or a solution of light malt extract just as easily.  The concentrations of sugars and such are the same.

Earlier, underfliptown, you were talking about sanitizing the outside of your syringe with alcohol.  That's cool and all, but what you should do instead is make a self-healing injection port and a tyvek breathing patch, so that way you're limited to bacteria that are inside the syringe.  With a good vendor and using only a single drop of spore solution you'll have about a 50% success rate, so make several LCs at the same time and do some small test jars.





Thank you for clarifying my awesomeness.

Take that NAH.

Score 1-1.

Your move sir.

:nicesmile:


--------------------
The ego is nothing other than the focus of conscious attention.


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InvisibleHypnotoadCroaked
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19155371 - 11/18/13 04:57 PM (10 years, 2 months ago)

I would advise as others have to research agar.  Not only can it accomplish exactly what a clean LC can, it can and will better your grasp on the hobby.

I have been doing agar work in pp5 plastic containers for a few months, and it has honestly been hands down the best investment of time to learn.  The biggest gain from working with agar is the ability to use clean cultures.  This alone makes it worth it.

Good luck in your future studies.


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OfflinePussyFart
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19155441 - 11/18/13 05:05 PM (10 years, 2 months ago)

Quote:

JMcDoogle said:
Thank you for clarifying my awesomeness.

Take that NAH.

Score 1-1.

Your move sir.

:nicesmile:



Ok, so I was wrong....I am not a robot guys...I am human.....I can be wrong.....

:unwanted:


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: PussyFart]
    #19155559 - 11/18/13 05:19 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Quote:

JMcDoogle said:
Thank you for clarifying my awesomeness.

Take that NAH.

Score 1-1.

Your move sir.

:nicesmile:



Ok, so I was wrong....I am not a robot guys...I am human.....I can be wrong.....

:unwanted:




You know our relationship, others may not.

We're okay, friend.

:douchewink:


--------------------
The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
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Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19155575 - 11/18/13 05:21 PM (10 years, 2 months ago)

Just make sure you guys post pics of your gay orgy...


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OfflinePussyFart
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19155655 - 11/18/13 05:33 PM (10 years, 2 months ago)

:curbyourenthusiasm:


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OfflineJMcDoogle
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Re: Simple question about potato dextrose [Re: underfliptown]
    #19155666 - 11/18/13 05:34 PM (10 years, 2 months ago)

Quote:

underfliptown said:
Just make sure you guys post pics of your gay orgy...




This isnt OTD.

Please refrain from saying things like that,

I find it offensive.


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The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
I suck and you should kill me


Registered: 03/07/11
Posts: 14,344
Last seen: 8 years, 5 months
Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19155677 - 11/18/13 05:36 PM (10 years, 2 months ago)

:cryaboutit:

I find you shitting on my thread offensive.

:dealwithit:


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 13 hours
Trusted Cultivator
Re: Simple question about potato dextrose [Re: underfliptown]
    #19155694 - 11/18/13 05:39 PM (10 years, 2 months ago)

Quote:

underfliptown said:
:cryaboutit:

I find you shitting on my thread offensive.

:dealwithit:



I like this guy.......he can stay....


Edited by PussyFart (11/18/13 05:40 PM)


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OfflineJMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 9 months
Re: Simple question about potato dextrose [Re: PussyFart]
    #19155710 - 11/18/13 05:42 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Quote:

underfliptown said:
:cryaboutit:

I find you shitting on my thread offensive.

:dealwithit:



I like this guy.......he can stay....




Meh, If you say so.

Again, reverse trolling works.

He's immune to it now.

Besides, I tried to help in the beginning, and you shat on me.

I than continued to help and you called me a liar.

Than you realized I was right and thanked me.

I guess we're okay/even.

:shrug:


--------------------
The ego is nothing other than the focus of conscious attention.


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Offlineunderfliptown
I suck and you should kill me


Registered: 03/07/11
Posts: 14,344
Last seen: 8 years, 5 months
Re: Simple question about potato dextrose [Re: JMcDoogle]
    #19155720 - 11/18/13 05:43 PM (10 years, 2 months ago)

:nyan:yay!:nyan:

I love it when everything comes full circle and we all love each other!!!!!!!


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