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sytar
Radiant



Registered: 09/01/13
Posts: 381
Last seen: 9 years, 5 months
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Pour vs no pour agar - advantages and disadvantages
#19152967 - 11/18/13 06:24 AM (10 years, 2 months ago) |
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I've seen some people here say that no pour agar is a crutch and that one would be far better off just learning how to pour the agar. However, I'm having a hard time finding specifics on why pouring the agar is so superior. One guess of mine would be that you can make many more Petri dishes a time with the pour method. However, it also seems to me that pouring leaves more room for contams to sneak in, even if you pour inside a SAB.
I bought a bunch of disposable Petri dishes that I'm guessing won't take well to being pressure cooked so I'm going to be pouring, but I was just curious about getting more info.
-------------------- I post from my phone. Excuse the typos and autocorrects.
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grainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
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Re: Pour vs no pour agar - advantages and disadvantages [Re: sytar]
#19152981 - 11/18/13 06:33 AM (10 years, 2 months ago) |
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No pour dishes are a little more difficult to work with due to the steep angle of the sides, but they're still a very viable means of using agar.
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Fred Teddy
Flying with Turkeys


Registered: 07/18/11
Posts: 208
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Re: Pour vs no pour agar - advantages and disadvantages [Re: grainbrain]
#19153062 - 11/18/13 07:39 AM (10 years, 2 months ago) |
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Petris have an advantage of size, easier to work with, quicker transfers, you can buy them pre-made, or you can whip up a bunch of em in a short time. That said, I use jars, lol. If using jars, learn to use a loop. Working with scalpels in a jar is miserable. A loop is waaaay easier to use in a jar than a scalpel. I have a dental pick I like to use, its not a loop per-say, but it works very well in jars. You can cut a wedge and stab it, or, I like to just rake some myc off the agar, like a loop.

Peace, FT
-------------------- ... its all about fun and games ... and no one has poked their eye out ... yet. Peace. Disclaimer: Fact is ... Its all fiction.
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Midnight Cyclone
StrangerDanger
Registered: 05/29/13
Posts: 399
Loc: oo ess aye oo ess aye
Last seen: 10 years, 11 days
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Re: Pour vs no pour agar - advantages and disadvantages [Re: grainbrain]
#19153084 - 11/18/13 07:50 AM (10 years, 2 months ago) |
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From what I know...
Advantages to no pour: -less room for contam -can use other (less expensive, reusable) items like baby food jars -don't necessarily need a laminar flow hood
Disadvantages to no pour: -limited on the number of plates you can PC at once -you can't PC most plastic petri dishes
Advantages to pouring your agar: -you can essentially pour unlimited plates -you can use disposable plates -you can use actual petri dishes instead of having to use baby food jars, or an alternate "petri dish" -once you can do this time and again while remaining sterile you can consider yourself pretty accomplished
Disadvantages to pouring your agar: -possible room for contam
EDIT:
Quote:
That said, I use jars, lol. If using jars, learn to use a loop. Working with scalpels in a jar is miserable. A loop is waaaay easier to use in a jar than a scalpel. I have a dental pick I like to use, its not a loop per-say, but it works very well in jars. You can cut a wedge and stab it, or, I like to just rake some myc off the agar, like a loop.
Ooo I like this Fred! I work in jars as well and am getting fed up with my scalpel. Luckily I have dental picks laying around because I use them as my dabber.
Edited by Midnight Cyclone (11/18/13 07:54 AM)
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SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
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Re: Pour vs no pour agar - advantages and disadvantages [Re: sytar] 1
#19153193 - 11/18/13 08:38 AM (10 years, 2 months ago) |
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Pros of no-pour: -Only have to open the jar to inoculate/transfer -Reusable -Don't have to wrap
Cons of no-pour: -steep angle makes it more difficult to transfer and isolate -can't see culture as well -have to remove and replace the lids every time
Pros of pouring: -takes up less space -clearer view of culture -easier to isolate/transfer -can pour several in one sitting -potentially exposes flaws in your sterile technique
Cons of pouring: -potentially exposes flaws in your sterile technique -creates plastic waste -must be wrapped
I can't really think of anything that would make me want to go back to no-pour
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