Home | Community | Message Board

Mycohaus
This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinesytar
Radiant
Male User Gallery


Registered: 09/01/13
Posts: 381
Last seen: 9 years, 5 months
Pour vs no pour agar - advantages and disadvantages
    #19152967 - 11/18/13 06:24 AM (10 years, 2 months ago)

I've seen some people here say that no pour agar is a crutch and that one would be far better off just learning how to pour the agar. However, I'm having a hard time finding specifics on why pouring the agar is so superior. One guess of mine would be that you can make many more Petri dishes a time with the pour method. However, it also seems to me that pouring leaves more room for contams to sneak in, even if you pour inside a SAB.

I bought a bunch of disposable Petri dishes that I'm guessing won't take well to being pressure cooked so I'm going to be pouring, but I was just curious about getting more info.


--------------------
I post from my phone. Excuse the typos and autocorrects.


Extras: Filter Print Post Top
Invisiblegrainbrain
Tribalistic

Registered: 05/11/11
Posts: 2,626
Re: Pour vs no pour agar - advantages and disadvantages [Re: sytar]
    #19152981 - 11/18/13 06:33 AM (10 years, 2 months ago)

No pour dishes are a little more difficult to work with due to the steep angle of the sides, but they're still a very viable means of using agar.


Extras: Filter Print Post Top
InvisibleFred Teddy
Flying with Turkeys


Registered: 07/18/11
Posts: 208
Re: Pour vs no pour agar - advantages and disadvantages [Re: grainbrain]
    #19153062 - 11/18/13 07:39 AM (10 years, 2 months ago)

Petris have an advantage of size, easier to work with, quicker transfers, you can buy them pre-made, or you can whip up a bunch of em in a short time. That said, I use jars, lol. If using jars, learn to use a loop. Working with scalpels in a jar is miserable. A loop is waaaay easier to use in a jar than a scalpel. I have a dental pick I like to use, its not a loop per-say, but it works very well in jars. You can cut a wedge and stab it, or, I like to just rake some myc off the agar, like a loop.



Peace,
FT


--------------------
... its all about fun and games ... and no one has poked their eye out ... yet.

Peace.

Disclaimer: Fact is ... Its all fiction.


Extras: Filter Print Post Top
OfflineMidnight Cyclone
StrangerDanger
Registered: 05/29/13
Posts: 399
Loc: oo ess aye oo ess aye Flag
Last seen: 10 years, 11 days
Re: Pour vs no pour agar - advantages and disadvantages [Re: grainbrain]
    #19153084 - 11/18/13 07:50 AM (10 years, 2 months ago)

From what I know...

Advantages to no pour:
-less room for contam
-can use other (less expensive, reusable) items like baby food jars
-don't necessarily need a laminar flow hood

Disadvantages to no pour:
-limited on the number of plates you can PC at once
-you can't PC most plastic petri dishes


Advantages to pouring your agar:
-you can essentially pour unlimited plates
-you can use disposable plates
-you can use actual petri dishes instead of having to use baby food jars, or an alternate "petri dish"
-once you can do this time and again while remaining sterile you can consider yourself pretty accomplished

Disadvantages to pouring your agar:
-possible room for contam

EDIT:

Quote:

That said, I use jars, lol. If using jars, learn to use a loop. Working with scalpels in a jar is miserable. A loop is waaaay easier to use in a jar than a scalpel. I have a dental pick I like to use, its not a loop per-say, but it works very well in jars. You can cut a wedge and stab it, or, I like to just rake some myc off the agar, like a loop.




Ooo I like this Fred! I work in jars as well and am getting fed up with my scalpel. Luckily I have dental picks laying around because I use them as my dabber. :bigblunt:


Edited by Midnight Cyclone (11/18/13 07:54 AM)


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Pour vs no pour agar - advantages and disadvantages [Re: sytar] * 1
    #19153193 - 11/18/13 08:38 AM (10 years, 2 months ago)

Pros of no-pour:
-Only have to open the jar to inoculate/transfer
-Reusable
-Don't have to wrap

Cons of no-pour:
-steep angle makes it more difficult to transfer and isolate
-can't see culture as well
-have to remove and replace the lids every time

Pros of pouring:
-takes up less space
-clearer view of culture
-easier to isolate/transfer
-can pour several in one sitting
-potentially exposes flaws in your sterile technique

Cons of pouring:
-potentially exposes flaws in your sterile technique
-creates plastic waste
-must be wrapped

I can't really think of anything that would make me want to go back to no-pour


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
Jump to top Pages: 1

PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* straight verm casing: advantages, disadvantages? comario2 2,214 6 10/07/02 12:21 PM
by zeta
* Grain to Agar? Aiko Aiko 2,470 12 05/23/16 11:00 AM
by bodhisatta
* question on agar Ripple 2,054 10 09/19/02 04:40 PM
by Ripple
* agar pouring apparatus? forevrgrounded98 1,053 5 05/12/04 02:52 AM
by ATWAR
* What are the advantages of..... NoFuture 644 4 06/29/03 01:58 PM
by Sorted
* some questions about agar ar393 996 3 06/11/01 03:04 PM
by Anno
* Re: Attn: ShroomGod, agar questions vivid 2,682 9 01/12/00 03:49 PM
by Anonymous
* agar
( 1 2 all )
mikearch 2,352 21 10/05/02 01:52 PM
by mikearch

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
3,019 topic views. 35 members, 226 guests and 38 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.007 seconds on 12 queries.