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Amphibolos
Le bourgeois gentilhomme




Registered: 05/22/09
Posts: 626
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Biohacking
#19152949 - 11/18/13 06:15 AM (10 years, 2 months ago) |
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Did someone ever went to Biocurious? I'm wondering what it look like as an organization.
The parallel with the early hackers and biohacking Article
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"Homo sum ; humani nihil a me alienum puto"
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Dest
Roller-Derby Coach


Registered: 06/14/09
Posts: 2,444
Last seen: 6 years, 5 months
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Dest
Roller-Derby Coach


Registered: 06/14/09
Posts: 2,444
Last seen: 6 years, 5 months
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Re: Biohacking [Re: Dest]
#19164068 - 11/20/13 09:13 AM (10 years, 2 months ago) |
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Amphibolos
Le bourgeois gentilhomme




Registered: 05/22/09
Posts: 626
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Re: Biohacking [Re: Dest]
#19165577 - 11/20/13 02:01 PM (10 years, 2 months ago) |
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It is indeed an interesting link,
The centrifuge machine is really interesting, especially since its a core equipment in every biotech lab. Along with the Thermocycler (open PCR machine)
Ill note these 2, i hope they give the blueprints for free 
Once our CNC will be done, these kind of projects will be easier to achieve. But how to mix an hackerspace/fablab with a DIY bio lab...Time will tell
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"Homo sum ; humani nihil a me alienum puto"
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dr.alkaline



Registered: 12/15/12
Posts: 684
Last seen: 4 years, 9 months
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I used to dream about doing this, but I think it is still out of reach for anyone trying to get into it as a hobby. Yes supply companies are making reagents cheaper and cheaper each year, but molec bio equip is still very expensive. You might be able to do it if you have high income, mush in the way some double income no kids couples can afford to be competitive in motorports, because that is the kind of money you are going to need.
A routine procedure like cloning will be a great example of how expensive this is going to be. High fidelity enzymes used in cloning are not cheap and run several hundred dollars for the smallest amount of units you can buy. NEB enzymes, plasmids, and selection antibiotics are going to add up. Column purification kits for PCR product cleaning and plasmid isolation are going to add up too. Purification columns for sequencing and the actual cost of sequencing is going to cost you quite a bit. Ligation enzyme and buffers for all the above are going to tack on quite a bit more. Storage is another problem and so is legal approval to do these things. Most of the equipment like centrifuge, gel boxes, and thermocyclers you can build yourself, but the cash this saves you will be a drop in the bucket.
Sadly, I dont think molec bio research is within reach of the citizen science. A yearly budget for an academic research lab can be hundreds of thousands to millions of dollars to do this type of research. Search NIH report for your local university and you will see what I am talking about.
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Metathetical
Stranger


Registered: 09/08/12
Posts: 34
Last seen: 7 years, 7 days
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I have been wondering how much it would cost to put something like this together. Relatively Cheap equipment like a thermocycler, pipettes, freezers, a basic centrifuge is available in liquidation auctions of big pharma labs. Reagents and enzyme would be expensive.
Basic cloning and protein engineering could probably be done in a basement or garage with a ~50,000$ USD lab setup. Plasmids and cDNAs are easy to find. I could imagine someone leaving school with their stack of freezer boxes also.
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dr.alkaline



Registered: 12/15/12
Posts: 684
Last seen: 4 years, 9 months
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I think for basic cloning you could do quite a few experiments for a couple thousand dollars. 50,000 is a high number.
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