Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds High THC Strains   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlinepsilohero
Stranger
Registered: 11/18/13
Posts: 41
Last seen: 8 years, 11 months
Some colonization issues..plz help
    #19152832 - 11/18/13 04:13 AM (10 years, 2 months ago)

Ok so Im having an issue right now I cant seem to solve. I have grown successfully in the past so Im familiar with the process.  I order fresh spores consisting of a B+ and Penis Envy Mix. I then prepared and inoculated some jars. Not a single B+ jar showed any signs of colonization but all of the PE showed at least some colonization. The following week I injected more B+ and PE. Again no growth on any B+ with growth again on the PE. When I opened the dull B+ jars from the first week they smelled almost like a bad wine (if that makes sense). I have grown B+ in the past with no issues and my technique is the same. The only thing I can even think is that maybe I received mislabeled B+ spores from a different species that cant colonize BRF cakes. Any input would be much appreciated, thx all


Extras: Filter Print Post Top
OfflineStromriderM
This must be the place
Male User Gallery


Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say... Flag
Last seen: 18 hours, 43 minutes
Trusted Cultivator
Re: Some colonization issues..plz help [Re: psilohero]
    #19152850 - 11/18/13 04:36 AM (10 years, 2 months ago)

Did you order the syringes from a reputable vendor?  By that I mean a shroomery sponsor


Extras: Filter Print Post Top
Offlinespacechildo
proletarians rise up
Male User Gallery


Registered: 01/24/13
Posts: 19,243
Loc: Babylon
Last seen: 6 years, 4 months
Re: Some colonization issues..plz help [Re: Stromrider]
    #19152885 - 11/18/13 05:18 AM (10 years, 2 months ago)

Sounds like the B+ syringe is bad. Did you get any mycel growth or just bacteria or mold?

Also what strom said. Lots of cowboys out there in the spore-vendor business.


Extras: Filter Print Post Top
Offlinepsilohero
Stranger
Registered: 11/18/13
Posts: 41
Last seen: 8 years, 11 months
Re: Some colonization issues..plz help [Re: Stromrider]
    #19153734 - 11/18/13 11:43 AM (10 years, 2 months ago)

*edit

Yes they are from a reputable source


Edited by psilohero (11/18/13 03:06 PM)


Extras: Filter Print Post Top
Offlinepsilohero
Stranger
Registered: 11/18/13
Posts: 41
Last seen: 8 years, 11 months
Re: Some colonization issues..plz help [Re: spacechildo]
    #19153748 - 11/18/13 11:46 AM (10 years, 2 months ago)

yes my thought was the problem lied within the syringes as well, as that is the only possible solution that could make sense in my head.  There is no visual mycelium growth or contamination growth or any kind. It looks just like a normal jar after sterilization with small needle slips under each lid hole and a slightly darker mixture hue where the spore liquid was injected.


Extras: Filter Print Post Top
OfflineAero
Orea


Registered: 11/01/13
Posts: 2,253
Last seen: 1 year, 2 months
Re: Some colonization issues..plz help [Re: psilohero]
    #19153816 - 11/18/13 12:05 PM (10 years, 2 months ago)

weird..contact your vendor, and tell him whats the situation, im sure ull get a refund


--------------------
SPREAD THE SPORES



Extras: Filter Print Post Top
Offlinepsilohero
Stranger
Registered: 11/18/13
Posts: 41
Last seen: 8 years, 11 months
Re: Some colonization issues..plz help [Re: Aero]
    #19153875 - 11/18/13 12:25 PM (10 years, 2 months ago)

lol but how do I tell him the situation without incrimination?


Extras: Filter Print Post Top
OfflineGiggle_Grower
A lil less noob each day
Male User Gallery


Registered: 04/07/10
Posts: 1,598
Loc: Shroomery
Last seen: 1 year, 5 months
Re: Some colonization issues..plz help [Re: psilohero]
    #19153903 - 11/18/13 12:35 PM (10 years, 2 months ago)

You didnt see any spores on your slides. Theses spores from vendors are for microscopy only. And you should prolly removed their name from the thread, so as too not incriminate them.


--------------------
I'm  always interested in trades.
The Awesome Purple Mystics
Noobs! The best tool here is up top to the right. Don't  forget about it. Just type your question in!:cool:
RR is my favorite source of knowledge. Check out his videos!

If I forgot to leave you a rating, please remind me.


Edited by Giggle_Grower (11/18/13 12:41 PM)


Extras: Filter Print Post Top
Offlinemonoculture
Mycaddict
Male User Gallery


Registered: 10/16/13
Posts: 246
Last seen: 9 years, 5 months
Re: Some colonization issues..plz help [Re: Giggle_Grower]
    #19155397 - 11/18/13 05:01 PM (10 years, 2 months ago)

When it smells like that I would say the syringe has a yeast contam.


Extras: Filter Print Post Top
Offlinepsilohero
Stranger
Registered: 11/18/13
Posts: 41
Last seen: 8 years, 11 months
Re: Some colonization issues..plz help [Re: monoculture]
    #19157122 - 11/18/13 09:57 PM (10 years, 2 months ago)

I realized the smell may be a result of the creek water I used in some of the jars. I will take note this weekend if I notice a difference in smell of the creek water and well water jars.  Water type doesnt seem to indicate any difference in colonization performance.

Recently I have also injected multiple different cube strains hoping to isolate those specific B+ syringes as the problem


Extras: Filter Print Post Top
InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Some colonization issues..plz help [Re: monoculture]
    #19157162 - 11/18/13 10:06 PM (10 years, 2 months ago)

Quote:

monoculture said:
When it smells like that I would say the syringe has a yeast contam.




Get the Vagasil :lol:

But seriously OP just email them and say that "after examination there does not appear to be any viable spores". This blanket statement will cover most situations and will not raise any alarms. Though one time I said that to a sponsor and they emailed me back suggesting that I re-hydrate the spores before inoculating my agar, and that they would send a replacement print anyway :lmafo:


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Unfolding Nature Unfolding Nature: Being in the Implicate Order   Kraken Kratom Red Vein Kratom   PhytoExtractum Kratom Powder for Sale   Original Sensible Seeds High THC Strains   Left Coast Kratom Buy Kratom Capsules   North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures


Similar ThreadsPosterViewsRepliesLast post
* Colonizing Help Plz Callampin 472 6 09/18/03 11:47 AM
by Callampin
* Quicky Colonization II (Please post responses!!!) Smokemon 3,365 8 05/26/15 07:19 PM
by R0B0G3N3S1S
* Issue with Myco-bag Air Exchange Supershrooomer 1,178 2 09/30/02 02:13 PM
by comario2
* colonization time related to amount of spores injected joeinc 887 2 05/26/03 12:51 AM
by lemunhed
* slow colonizing Thai cubies - any ideas? jimsuzo 734 4 04/12/03 09:58 AM
by jimsuzo
* Jars with black spots(mold?) continue to colonize?!?!?
( 1 2 3 all )
vitamink 12,584 46 06/30/03 10:19 PM
by vitamink
* Help: top of jar colonizing sloow FungusFarmer 1,863 3 09/06/01 10:15 AM
by puscle
* Straw Casing , colonizing and contams , plz help Callampin 1,677 10 12/23/03 09:05 AM
by trkgsy

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
674 topic views. 26 members, 235 guests and 32 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.01 seconds on 14 queries.