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underfliptown
I suck and you should kill me


Registered: 03/07/11
Posts: 14,344
Last seen: 8 years, 5 months
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Re: What's the best mushroom strain? [Re: bodhisatta]
#19152122 - 11/17/13 10:02 PM (10 years, 2 months ago) |
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Yes, i bought 2 spore syringes. I can make a LC from the best fruits from either of these.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: What's the best mushroom strain? [Re: underfliptown]
#19152160 - 11/17/13 10:11 PM (10 years, 2 months ago) |
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Quote:
underfliptown said: Yes, i bought 2 spore syringes. I can make a LC from the best fruits from either of these.
skip the LC
Put your clones to agar. Then you'll be able to see if they're clean and you won't have to use a needle to inject your jars. You're going to need the same amount of good sterility(sterile tek) to make a clean LC from a clone anyway, just as clean(SAB or LFH ,sterile tek) as it has to be to do agar.
Edited by Trusted cuItivator (11/17/13 10:12 PM)
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underfliptown
I suck and you should kill me


Registered: 03/07/11
Posts: 14,344
Last seen: 8 years, 5 months
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Re: What's the best mushroom strain? [Re: bodhisatta]
#19152195 - 11/17/13 10:17 PM (10 years, 2 months ago) |
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Quote:
bodhisatta said:
Quote:
underfliptown said: Yes, i bought 2 spore syringes. I can make a LC from the best fruits from either of these.
skip the LC
Put your clones to agar. Then you'll be able to see if they're clean and you won't have to use a needle to inject your jars. You're going to need the same amount of good sterility(sterile tek) to make a clean LC from a clone anyway, just as clean(SAB or LFH ,sterile tek) as it has to be to do agar.
I hear ya man, but i have no access to agar and I hate buying things online. It's too expensive. I am gonna start with LC and when I get some extra money I am gonna move onto agar if I feel teh need.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: What's the best mushroom strain? [Re: underfliptown]
#19152211 - 11/17/13 10:20 PM (10 years, 2 months ago) |
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Might as well stick with spores then, just make more spore prints from your fruits and then syringes. You're going to need a lot of spores if you want to do LC since so many will most likely contam you'll want to make as many as possible and then test them on a single cake to see if they're viable,
Edited by Trusted cuItivator (11/17/13 10:21 PM)
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underfliptown
I suck and you should kill me


Registered: 03/07/11
Posts: 14,344
Last seen: 8 years, 5 months
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Re: What's the best mushroom strain? [Re: bodhisatta]
#19152221 - 11/17/13 10:23 PM (10 years, 2 months ago) |
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Quote:
bodhisatta said: Might as well stick with spores then, just make more spore prints from your fruits and then syringes. You're going to need a lot of spores if you want to do LC since so many will most likely contam you'll want to make as many as possible and then test them on a single cake to see if they're viable,
No way dude. You just use a tissue sample from your best cluster and use that to innoc your LC.
Sterility for that isn't hard at all.
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OgreLokon
Pretty Fun Guy


Registered: 06/30/13
Posts: 512
Last seen: 6 years, 5 months
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Re: What's the best mushroom strain? [Re: bodhisatta]
#19152226 - 11/17/13 10:24 PM (10 years, 2 months ago) |
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You could also check an Asian market for agar-agar, if you don't like ordering things online...
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underfliptown
I suck and you should kill me


Registered: 03/07/11
Posts: 14,344
Last seen: 8 years, 5 months
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Re: What's the best mushroom strain? [Re: OgreLokon]
#19152234 - 11/17/13 10:26 PM (10 years, 2 months ago) |
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and have to deal with all the asians.... lol
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: What's the best mushroom strain? [Re: underfliptown]
#19152235 - 11/17/13 10:27 PM (10 years, 2 months ago) |
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Quote:
underfliptown said:
Quote:
bodhisatta said: Might as well stick with spores then, just make more spore prints from your fruits and then syringes. You're going to need a lot of spores if you want to do LC since so many will most likely contam you'll want to make as many as possible and then test them on a single cake to see if they're viable,
No way dude. You just use a tissue sample from your best cluster and use that to innoc your LC.
Sterility for that isn't hard at all.
Ok man, I've been there done that, but make your own mistakes and setbacks. You'll see contams all the time when you work with agar and clone tissue, but you'll be able to have success in cleaning up the cultures. All those contams i routinely see even with inside the stem tissue would destroy a whole LC. On a plate they're not so devastating. Like I said you'll want to make a lot of LC's if you want to try that route, and a lot of test cakes too.
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poopy mcpooperson
Pogonophobia



Registered: 10/17/13
Posts: 124
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Re: What's the best mushroom strain? [Re: underfliptown]
#19152236 - 11/17/13 10:27 PM (10 years, 2 months ago) |
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LCs are a really bad way to keep a constant clean amount of mycelium. i would suggest you make around 6 quarts of LC if you are dead set on doing LC. a few will inevitably become contaminated. best of luck to you though.
-------------------- love and laughter
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Just moving tissue around in a SAB there's a contam spot on one of the three receiving dishes. I was still able to use that dish but if it were an LC it would have destroyed everything you inoculated with it. Moving clone tissue into a LC jar even in a SAB you can expect a good failure rate.
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Prisoner#1
Even Dumber ThanAdvertized!


Registered: 01/22/03
Posts: 193,665
Loc: Pvt. Pubfag NutSuck
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Re: What's the best mushroom strain? [Re: underfliptown]
#19152785 - 11/17/13 10:31 PM (10 years, 2 months ago) |
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This thread has been closed.
Reason: .
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