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OfflineDelay
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Registered: 10/29/13
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Pleurotus Eryngii Contamination??
    #19150080 - 11/17/13 02:40 PM (10 years, 2 months ago)

Hi!

I recently purchased a culture of what pleurotus eryngii from themushrompath.com. I would not recommend this company. The syringe appeared loaded with mycelium, so I went ahead and inoculated some jars. They all became contaminated with slime mold. I later noticed that the same slime mold appeared to be covering the inside walls of the syringe, where there was air. It's worth noting identical substrate from the same batch of pressure cooked jars colonized fine with other cultures, under the exact same conditions/procedures. I'm fairly certain the original culture was contaminated.

The failed jars were done using wheat grains with small amounts of coffee. I had an extra jar laying around with 2-row brewers malt (barley) grain, so I thought what the heck and inoculated it with the pleurotus eryngii culture. To my surprise, I cannot see any slime mold growing. This white mycelium started growing about 4 or 5 days ago. Does this look like pleurotus eryngii, or a contam? My rationale is that perhaps the brewers malt substrate does not favor the slime mold as did the wheat/coffee substrate, hence this different culture growing.



Thanks. Hope that wasn't too confusing.


Edited by Delay (11/17/13 02:41 PM)


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OfflineDelay
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Re: Pleurotus Eryngii Contamination?? [Re: Delay]
    #19159870 - 11/19/13 02:15 PM (10 years, 2 months ago)

I just checked today, and there appears to be some white whispy mycelium which developed that looks more like pleurotus based off my limited experience.

I'm assuming that the bright white patches are indeed a contamination? Any input would be appreciated.


I'm thinking that maybe I should try and clone the whispy pleurotus-looking myc and assume defeat for this jar? Or should I just chuck it?

~Delay


Edited by Delay (11/19/13 02:16 PM)


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OfflineKizzle
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Re: Pleurotus Eryngii Contamination?? [Re: Delay]
    #19160573 - 11/19/13 04:44 PM (10 years, 2 months ago)

Probably contaminated. I haven't grown that particular species before though so I could be wrong.


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