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Delay
Stranger

Registered: 10/29/13
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Why is recovery needed for GLC?
#19147771 - 11/16/13 11:13 PM (10 years, 2 months ago) |
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Hi,
I have searched for this answer but I cannot seem to find it. Why does one need the mycelium to recover in the syringe with the GLC tek? Wouldn't the mycelium recover on grain just as well as in the syringe?
Intuitively, I would think that immediately inoculating after extracting mycelium-laden water would be preferred, as the mycelium wouldn't have to recover twice (in syringe, then after inoculation with >48 hour old water.
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OgreLokon
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Re: Why is recovery needed for GLC? [Re: Delay]
#19147774 - 11/16/13 11:14 PM (10 years, 2 months ago) |
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tripdawg420
low life with no life



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Re: Why is recovery needed for GLC? [Re: OgreLokon]
#19148074 - 11/17/13 12:45 AM (10 years, 2 months ago) |
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yup you can use the glc immediatly
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Violet



Registered: 12/06/11
Posts: 4,205
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Re: Why is recovery needed for GLC? [Re: tripdawg420]
#19148633 - 11/17/13 07:12 AM (10 years, 2 months ago) |
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Yeah there's no need to wait for using the GLC.
However "recovery" time on the grains will be several days anyway. This is because the mycelium is not so much recovering as establishing itself in the surface of the grains for the first time, before growth away from the grains will begin becoming visible. With a G2G inoc, mycelium is already established on those initial grains, trimming a day or two off.
-------------------- Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it! PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers The simplest, quickest, safest tek! For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers! • Violet's Teks and Posts •
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InTheBiggun
The Milk-Man



Registered: 06/01/09
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Re: Why is recovery needed for GLC? [Re: Violet]
#19148873 - 11/17/13 09:23 AM (10 years, 2 months ago) |
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I do quart jars 2/3's filled using 10cc of GLC, after a good rolling around they fully colonize in 4 days.
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InTheBiggun
The Milk-Man



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Re: Why is recovery needed for GLC? [Re: InTheBiggun]
#19149146 - 11/17/13 10:49 AM (10 years, 2 months ago) |
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After four days:
On the left heavy g2g On the right 5cc GLC In the middle 10cc GLC
The G2G did take off first, but the 10cc GLC was much faster in the end.
Edited by InTheBiggun (11/17/13 10:50 AM)
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Psilicon
Really Nice Guy


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Re: Why is recovery needed for GLC? [Re: InTheBiggun]
#19149442 - 11/17/13 12:06 PM (10 years, 2 months ago) |
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Okay, but with popcorn you can't just say it colonizes in four days. There's way less ground for the mycelium to cover than with other grains.
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InTheBiggun
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Re: Why is recovery needed for GLC? [Re: Psilicon]
#19149498 - 11/17/13 12:22 PM (10 years, 2 months ago) |
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It's worked the same with popcorn, wbs and other grains. The important thing is to roll/shake it well to distribute mycelium everywhere.
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Psilicon
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Re: Why is recovery needed for GLC? [Re: InTheBiggun]
#19149526 - 11/17/13 12:29 PM (10 years, 2 months ago) |
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I'm a little skeptical if what you're telling me is that your popcorn and your WBS colonize at the same rates.
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InTheBiggun
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Re: Why is recovery needed for GLC? [Re: Psilicon]
#19149563 - 11/17/13 12:36 PM (10 years, 2 months ago) |
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Its not the "4 day rule", I wasn't saying it was the exact same growth for all or any grain...
My point was to the OP that there is no need to allow GLC syringes recover, in my opinion, and that the GLC'd jars, regardless of grain type move very fast(when inoculated with enough GLC), even when side by side with G2G jars.
Feel free to be skeptical, or call bullshit. I use GLC all the time on various grains and I'm speaking from my own personal experiences.
Edited by InTheBiggun (11/17/13 12:41 PM)
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Psilicon
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Re: Why is recovery needed for GLC? [Re: InTheBiggun]
#19149588 - 11/17/13 12:40 PM (10 years, 2 months ago) |
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No, it's not that I disagree with anything you're saying here. I just think it's misleading to say:
Quote:
InTheBiggun said: I do quart jars 2/3's filled using 10cc of GLC, after a good rolling around they fully colonize in 4 days.
when those jars are full of popcorn, which colonizes disproportionately fast because it's got very few very huge kernels in a jar.
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InTheBiggun
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Re: Why is recovery needed for GLC? [Re: Psilicon]
#19149668 - 11/17/13 01:02 PM (10 years, 2 months ago) |
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The thing I do is drain my soaked grains very well before PC'ing, by putting a plate on the colander and "whipping" the last of the trapped water out.
If its small grains I use a piece of window screen inside the colander.
The reason I do this is because 10cc of GLC is a lot of extra moisture if your grains are already extra-wet. I noticed right away that 10cc colonizes a lot faster than 5cc.
You could use verm at the bottom, but I don't myself.
Of course you could use 5cc in a pint... I was only speaking of quart jars (with room for mixing).
Edited by InTheBiggun (11/17/13 01:03 PM)
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Delay
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Re: Why is recovery needed for GLC? [Re: InTheBiggun]
#19150125 - 11/17/13 02:48 PM (10 years, 2 months ago) |
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Interesting. I am growing a very slow species that a friend gifted me, but I think my observations are valid nonetheless...I'm quite a novice so perhaps I am overlooking some abiotic factor, such as moisture content, but these are my observations thus far:
-I underestimated how much GLC to use in my first attempt, adding ~0.5cc/Liter of substrate. A MONTH later, I am seeing the first growth of what appears to be my culture.
-Interestingly, adding a similar ratio of GLC/grain after 48 hours of recovery yielded much better results (fully colonized after 5 weeks).
I just inoculated some jars with 10CC of fresh GLC. Interested to see the results.
Perhaps the recovery time (in syringe) is more beneficial if you are using a low GLC/grain ratio, but negligible if inoculating with greater quantities of GLC???
Edited by Delay (11/17/13 02:52 PM)
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InTheBiggun
The Milk-Man



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Re: Why is recovery needed for GLC? [Re: Delay]
#19150258 - 11/17/13 03:20 PM (10 years, 2 months ago) |
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"Shake" well... roll em around good. A few hours later if there is pooling water roll that around to. Get that myc everywhere.
What sprcies? Seems that GLC should grow much, much faster than what you mention.
What kind of jar did the GLC come from? I've noticed aged mycelium that is milked of GLC can perform quite slowly.
Edited by InTheBiggun (11/17/13 03:23 PM)
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Delay
Stranger

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Re: Why is recovery needed for GLC? [Re: InTheBiggun]
#19150336 - 11/17/13 03:40 PM (10 years, 2 months ago) |
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Mhmmm yes that was probably the issue. It was a species in the Sclerotiniaceae family, and at least 4 weeks into sclerotia formation...Furthermore, I didn't shake the jar as I did not want to disturb the mycelium/stone formation. I got around the shaking issue by injecting sterilized water into my parent jar as forcefully as possible, so as to dislodge a minimal amount of mycelium via the force of the water hitting the colonized substrate.
Nonetheless, I noticed a very significant difference between GLC which recovered in the syringe vs. in the jars. Again, I suspect the benefit of allowing it to recover in a syringe might be exaggerated when using a low amount of GLC/grain, or a weakly mycelium-laden GLC. I couldn't and still don't understand why that might be the case. My observations are clearly contrary to what others observed at higher inoculation rates.
Again, the moisture content of the jars that I inoculated on the same day vs. 48 hours later may have differed, which from what I gather could fully or in part explain the differences in colonization times. These are just crude and unintentional observations of mine. A proper experimental design would be needed to determine whether there is a correlation between inoculation rate and the benefit of recovering the mycelium in the syringe, I would think.
Edited by Delay (11/17/13 03:44 PM)
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InTheBiggun
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Re: Why is recovery needed for GLC? [Re: Delay]
#19150454 - 11/17/13 04:02 PM (10 years, 2 months ago) |
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I've done lots of experiments with GLC mycelium milking in all kinds of scenarios and inoc-rates, even side-by-sides with G2G at different rates... but mostly with Cubensis although this teknique works equally as good or better with other species, especially edibles (Oyster, Shiitake etc), and aggressive species like woodlovers.
Good luck by the way...
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InTheBiggun
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Re: Why is recovery needed for GLC? [Re: InTheBiggun]
#19150946 - 11/17/13 05:46 PM (10 years, 2 months ago) |
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One of the ways I make GLC is to start cultures/spores in small agar-jars, let that colonize, observe and transfer if necessary.

When that's colonized I add sterilized grain.

That colonizes quickly...

When that's done I inject a certain amount of water into each agar/grain jar, shake vigorously and suck it back.

That's how I do GLC on a small scale. The jars recovery very quickly and can be milked more than a dozen times.
For larger projects I developed a mycelium milking system using jar lids.

This unit is sterilized and then hooked onto a colonized grain jar, inside a SAB/clean area. That is flipped and hooked onto a clean-water jar. Thats tightened and then flipped to flood the grain jar. Thats shaken vigorously and then flipped to remove the mycelium water.

This is how I collect as much GLC as I need...
If I milk two jars of grain into one collection jar, that double strength myc-water turns a deep shade of blue.
Anyhow, just sharing a way of doing GLC.
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Delay
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Re: Why is recovery needed for GLC? [Re: InTheBiggun] 1
#19175177 - 11/22/13 03:00 PM (10 years, 2 months ago) |
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That's pretty cool. What is underneath the hose clamp, sealing it off?
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InTheBiggun
The Milk-Man



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Re: Why is recovery needed for GLC? [Re: Delay]
#19175565 - 11/22/13 04:44 PM (10 years, 2 months ago) |
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The two lid bands are glued together with waterproof glue (gorilla glue), pressed down together at the same time the hose clamp is tightened.
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