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illuminati
Strange


Registered: 06/17/07
Posts: 556
Loc: Aboard the T.A.R.D.I.S.
Last seen: 3 years, 4 months
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Contamination After 90%+ Colonization
#19144479 - 11/16/13 08:00 AM (10 years, 2 months ago) |
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Out of 30 WBS jars inoculated with agar wedges, only 5 remain. The 25 that contaminated, did so after nearly full colonization. The jars have been colonizing in a TiT incubator due to the ambient temperature being around 50F; the incubator was cleaned with bleach prior to the jars being loaded in.
Other information:
- The agar being used is from a plate that has been grown to fruit, and has not exhibited these issues before, but I'm not ruling this out as the culprit.
- The WBS jars were prepared by soaking grains for 12 hours, rinsing well, drying for approximately 14 hours (until at field capacity). They were loaded into quart mason jars with a Tyvek filter and covered with foil. The jars were PC'd at 15PSI for 1HR, at which time the heat was turned off, and the PC was left on the stove to cool.
- Some of the jars had appeared to be fully colonized with the exception of a few grains at the top. When left to sit longer, occasionally these jars would form green spots (what I believe to be trichoderma) over the mycelium.
If anyone can shed some light on this or provide any thoughts as to why this may be happening, it would be greatly appreciated!
-------------------- I didn't get turned on I just got turned I wasn't as aroused as I was concerned for each one of 'em I've hurt and every time I've been burned I've got a lot to teach but even more to learn
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Kizzle
Misanthrope


Registered: 08/30/11
Posts: 9,855
Last seen: 14 hours, 25 minutes
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Re: Contamination After 90%+ Colonization [Re: illuminati]
#19145179 - 11/16/13 12:51 PM (10 years, 2 months ago) |
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A few possibilities -
You let your agar fully colonize, the mycelium can become contaminated if it starts growing up the side of the dish The culture became contaminated during a transfer You're filters were wet or damaged
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illuminati
Strange


Registered: 06/17/07
Posts: 556
Loc: Aboard the T.A.R.D.I.S.
Last seen: 3 years, 4 months
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Re: Contamination After 90%+ Colonization [Re: Kizzle]
#19145207 - 11/16/13 12:59 PM (10 years, 2 months ago) |
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But do any of these things account for the fact the jars are almost entirely colonized before they contaminate? I feel like any of the factors you mentioned would cause contamination much earlier.
-------------------- I didn't get turned on I just got turned I wasn't as aroused as I was concerned for each one of 'em I've hurt and every time I've been burned I've got a lot to teach but even more to learn
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