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hbettag
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Registered: 06/30/13
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noob exprimental bulk substrate question?????
#19136130 - 11/14/13 01:09 PM (10 years, 2 months ago) |
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Ok, so I wonder can I experiment with substrate? I know that question probably sounds stupid but here why I ask. I have spent countless hours doing research into mycology. And it seems as though there are only a small hand full of bulk substrate recipes. Now I know this is partially because they are the tried true proven methods they work they been tested hence they are repeated. I have some hpoo, some brf, some wbs, some peat if I were to say mix all providing that my moisture content was right, and I sterilize correctly could inoculate. I haven't seen too much in the way of experimenting with this. So if I do some kind of crazy concoction what happens? Has anyone else tried this and wish to share?
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: noob exprimental bulk substrate question????? [Re: hbettag]
#19136289 - 11/14/13 01:59 PM (10 years, 2 months ago) |
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Bulk substrates like coir and Hpoo are relatively low nutrient. If you used something like BRF it would contaminate before your spawn(mycellium) had a chance to colonize the bulk substrate. Especially if you sterilize your bulk substrate. We never sterilize a substrate except in rare circumstances. If you're spawning in open air(99% of the time) your substrate needs to be pasteurized so that thermophillic bacteria colonize it and repel contamination that would get to the substrate before your mycellium does.
Spawn : sterilize high nutrient source (WBS, Rye, etc) The mycellium colonizes this in a sterile vessel usually a bag or jar that's been pressure cooked.
This spawn acts as 1000s of tiny inoculation points for a bulk substrate. if you shoot spores into bulk substrate it will never colonize. We use spawn to jump start that process.
Bulk substrate is inoculated with spawn and we call that spawning to bulk. If the bulk is sterilized any contams in the open air will take over it. Pasteurized bulk is resistant to contamination yet the mycellium can still eat it.
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hbettag
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Re: noob exprimental bulk substrate question????? [Re: bodhisatta]
#19136379 - 11/14/13 02:29 PM (10 years, 2 months ago) |
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I know what your saying, let me be a little more specific. Ok so made up I dont know 15-20 pints brf jars pf tek recipe, had roughly 1.5 qt leftover it went into bucket, several weeks later made up 10-15 qt wbs, had 2-3 qts left threw it in same bucket, mixed up bunch of casing mix had some verm/peat leftover it went in bucket. Pasteurized some hpoo had some left over went in bucket,,,,So all of a sudden got this 5 gallon bucket full of everything, what happens if I fill some say pint jars and sterilize, i mean providing my moisture content is right then whats the problem? I mean look at Hippies supermix or something like that just wondering what will happen?
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SpitballJedi
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Registered: 10/13/12
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Re: noob exprimental bulk substrate question????? [Re: hbettag] 1
#19136833 - 11/14/13 04:03 PM (10 years, 2 months ago) |
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Deleted---oops, I misread. Good eye cronicr.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
Edited by SpitballJedi (11/14/13 05:31 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: noob exprimental bulk substrate question????? [Re: hbettag]
#19137252 - 11/14/13 05:26 PM (10 years, 2 months ago) |
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Quote:
hbettag said: I know what your saying, let me be a little more specific. Ok so made up I dont know 15-20 pints brf jars pf tek recipe, had roughly 1.5 qt leftover it went into bucket, several weeks later made up 10-15 qt wbs, had 2-3 qts left threw it in same bucket, mixed up bunch of casing mix had some verm/peat leftover it went in bucket. Pasteurized some hpoo had some left over went in bucket,,,,So all of a sudden got this 5 gallon bucket full of everything, what happens if I fill some say pint jars and sterilize, i mean providing my moisture content is right then whats the problem? I mean look at Hippies supermix or something like that just wondering what will happen?
if it's all going into the bucket dry, how do you get the moisture content right? it takes X amount of time to get grains hydrated which is X amount of time longer then it takes to get brf hydrated and X amount of time for bulk subs, your flour would be sticky your grains would be dry and bottom line it sounds like a mess waiting to happen, but mixing is not old news provided it's done correctly but is by no means a smart approach
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,889
Loc: Milky way
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Re: noob exprimental bulk substrate question????? [Re: hbettag]
#19137492 - 11/14/13 06:25 PM (10 years, 2 months ago) |
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Quote:
hbettag said: I know what your saying, let me be a little more specific. Ok so made up I dont know 15-20 pints brf jars pf tek recipe, had roughly 1.5 qt leftover it went into bucket, several weeks later made up 10-15 qt wbs, had 2-3 qts left threw it in same bucket, mixed up bunch of casing mix had some verm/peat leftover it went in bucket. Pasteurized some hpoo had some left over went in bucket,,,,So all of a sudden got this 5 gallon bucket full of everything, what happens if I fill some say pint jars and sterilize, i mean providing my moisture content is right then whats the problem? I mean look at Hippies supermix or something like that just wondering what will happen?
After a couple weeks of having hydrated PF mix, WBS in a bucket how is not a festering pile of contamination. If you make a PF jar and leave it sit for 3-4 days and forget to sterilize it it's going to look like a rainbow took a shit inside of the jar.
And
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hbettag
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Re: noob exprimental bulk substrate question????? [Re: bodhisatta]
#19271928 - 12/13/13 06:18 PM (10 years, 1 month ago) |
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Ok so apparently it does work, experiment was putting all leftover ingredients into one bulk mixture, pplacing into jars pressure cook at 15psi 2.5 hrs heres pic of result after 6 days got about a 2inch line all the way from top to bottom of jar
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 8 days
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Re: noob exprimental bulk substrate question????? [Re: hbettag]
#19271989 - 12/13/13 06:33 PM (10 years, 1 month ago) |
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--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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hbettag
Stranger


Registered: 06/30/13
Posts: 116
Last seen: 6 years, 4 months
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Re: noob exprimental bulk substrate question????? [Re: cronicr]
#19272177 - 12/13/13 07:36 PM (10 years, 1 month ago) |
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Here's how I did the moisture, so anyway I got a pillow case, went outside to my bucket put about 2 gallons worth of mixture (wbs,coir,poo,brf,coffee grounds) into the pillow case took my 16 qt PC placed on stove filled 3/4 with filtered tap water placed pillow case in PC lid off heated water to 165 maintained just over two hours, took rack out of oven placed it onto bucket in bathtub, took pillow case set it onto rack to drain overnight, next day squeezed excess moisture from bag.
Now some of this fresh pasteurized creation went into small tupperware trays to be used right then as bulk, the rest was put into jars and sterilized to see if I could inoculate with live culture attached pic is one of said jars
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