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Offlineryanferg
Stranger
Registered: 08/09/13
Posts: 1
Last seen: 1 year, 5 months
lab sterilization
    #19099267 - 11/07/13 08:09 AM (10 years, 3 months ago)

I am building a small lab in my basement. I will most likely not be using a flow hood. Will UVC lights be sufficient? Or would anyone recommend this as a viable option,

http://www.amazon.com/PetAirapy-PA-777-Portable-Purifier-Ionizer/dp/B002RL9BBO/ref=sr_1_4?ie=UTF8&qid=1383836002&sr=8-4&keywords=air+disinfector

I will also be using a glove box.

many thanks!


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Invisible36fuckin5
Alchemycologist


Registered: 08/11/03
Posts: 12,081
Loc: Diving into Mystical Territori...
Trusted Cultivator
Re: lab sterilization [Re: ryanferg]
    #19099567 - 11/07/13 09:45 AM (10 years, 3 months ago)

Both those options are ridiculous.

Build a flowhood and use it, and skip all the dumb shit.


--------------------
Redd Foxx said:
If you're offended I don't give a shit and don't come see me no more.

Pat The Bunny said:
A punk rock song won't ever change the world, but I can tell you about a couple that changed me.

bodhisatta said:
i recommend common sense and figuring it out.

These are the TEKs I use. They're all as cheap and easy as possible, just like your mom.


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 11 days
Trusted Cultivator
Re: lab sterilization [Re: ryanferg]
    #19099571 - 11/07/13 09:46 AM (10 years, 3 months ago)

Quote:

ryanferg said:
I am building a small lab in my basement. I will most likely not be using a flow hood. Will UVC lights be sufficient? Or would anyone recommend this as a viable option,

http://www.amazon.com/PetAirapy-PA-777-Portable-Purifier-Ionizer/dp/B002RL9BBO/ref=sr_1_4?ie=UTF8&qid=1383836002&sr=8-4&keywords=air+disinfector

I will also be using a glove box.

many thanks!



None of the above.

No UV lights, no air filters, no glove boxes.

If a Laminar Flow Hood is not an option, the next best thing would be a SAB(Still Air Box).

This is a tub with 2 arm holes cut/melted out, and maybe tyvek sleeves.

Gloves do not get attached to this box, you wear the gloves.

SABs are better than GBs for this hobby.


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OfflineAmanita virosa
botanist by day
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Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
Trusted Cultivator
Re: lab sterilization [Re: PussyFart]
    #19127120 - 11/12/13 03:56 PM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Quote:

ryanferg said:
I am building a small lab in my basement. I will most likely not be using a flow hood. Will UVC lights be sufficient? Or would anyone recommend this as a viable option,

http://www.amazon.com/PetAirapy-PA-777-Portable-Purifier-Ionizer/dp/B002RL9BBO/ref=sr_1_4?ie=UTF8&qid=1383836002&sr=8-4&keywords=air+disinfector

I will also be using a glove box.

many thanks!



None of the above.

No UV lights, no air filters, no glove boxes.

If a Laminar Flow Hood is not an option, the next best thing would be a SAB(Still Air Box).

This is a tub with 2 arm holes cut/melted out, and maybe tyvek sleeves.

Gloves do not get attached to this box, you wear the gloves.

SABs are better than GBs for this hobby.



I suggest scratching the word "maybe" from in front of the word tyvek sleeves. They are cheap, effective, can be autoclaved, reused over and over, and will make your SAB complete.


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
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Re: lab sterilization [Re: Amanita virosa]
    #19127133 - 11/12/13 03:59 PM (10 years, 3 months ago)

I only said maybe because it's not really needed....

But I wear a pair and have a pair attached to my box so lol.


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InvisiblecArcace-x
YeAh!!
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Registered: 10/17/13
Posts: 2,412
Loc: The Simulator
Re: lab sterilization [Re: ryanferg]
    #19131130 - 11/13/13 12:48 PM (10 years, 3 months ago)

i did my lab in the basement too but due to the high risk of contam. with mold and evil, i made a small closed room with some black'n'white wrap, its easy to keep clean and you can make it almost air-tight with some duck-tape and velcro. a simple SAB  work fine in it. :wink:

a flow hood in a dirty basement is useless
Good Luck


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Edited by cArcace-x (11/13/13 01:49 PM)


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Offlinenanncee
Male


Registered: 12/01/12
Posts: 434
Loc: Utah
Last seen: 9 years, 2 months
Re: lab sterilization [Re: cArcace-x]
    #19135418 - 11/14/13 09:48 AM (10 years, 3 months ago)

Quote:

cArcace-x said:

a flow hood in a dirty basement is useless





That is completely false.. We used a flowhood in a lived in bedroom covered in carpet, bedding and clothing for 2 years. If you are good at culturing it doesn't matter where the flowhood is. Go look at what Sparkle is doing..

I haven't added up numbers recently but once we learned what we were doing our contam rate was under %10.

Im not saying it is the ideal conditions to use a flowhood in, but it is completely acceptable. Just plan on having a higher contam rate until you figure stuff out.


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Edited by nanncee (11/14/13 09:56 AM)


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InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
Re: lab sterilization [Re: nanncee]
    #19135464 - 11/14/13 09:58 AM (10 years, 3 months ago)

I've been outa the scene for awhile so bear with me, but I'm a little shocked at the negative points given to GB's over a SAB. Why am I not seeing what's so much better about a box with holes and hands pumping in and out compared to a sealed box which is actually more of a SAB than a flexible holy rubbermaid and dish washing gloves? Please fill me in.


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OfflinePussyFart
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Re: lab sterilization [Re: NOS4A2]
    #19135519 - 11/14/13 10:09 AM (10 years, 3 months ago)

Quote:

NOS4A2 said:
I've been outa the scene for awhile so bear with me, but I'm a little shocked at the negative points given to GB's over a SAB. Why am I not seeing what's so much better about a box with holes and hands pumping in and out compared to a sealed box which is actually more of a SAB than a flexible holy rubbermaid and dish washing gloves? Please fill me in.



When you "Seal" and attach gloves to a box, it makes it impossible to flame sterilize a tool outside the box.

Flame sterilizing inside the box is just silly, as the heat currents will negate the "still air environment.

Also, with gloves attached to the box, every time you move your arms air will get sucked in and out the lid, which is extremely hard to completely seal.

THis also negates the "still air" environment.

A SAB with gloves attached to you, rather than the box gives you the freedom you need....

SABs are just all around better.


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InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
Re: lab sterilization [Re: PussyFart]
    #19135586 - 11/14/13 10:31 AM (10 years, 3 months ago)

Ok, I can see your points. I don't mean to beat this to death, yet I believe if your not making a flimsy GB out of totes but something substantial like 3/4" plywood and include a thick foam seal with clamping devices you can eliminate ALL outside air exchange. It's not as if it's a pressurized container where there's no room to displace the volume of two hands unless you're working in a very small box. As far as the flaming, I see that as a matter of opinion. In my opinion a tool sterilized outside will have to travel through the outside atmosphere however short as your hands push air inside as well which can contaminate your still air environment just the same as an ill aimed butane pencil torch inside a large GB that never opens and has been redundantly sterilized and let to settle. When you first use a GB you find out real quick that the result of having a conventional alcohol lamp constantly burning in a GB can be a very sketchy proposition. But I'm not going to argue that you're wrong by any means. You have obviously plenty of experience which has taught you all to appreciate the merits of a SAB over most GB's and I respect that.
:smile:


--------------------


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OfflineAmanita virosa
botanist by day
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Registered: 12/04/11
Posts: 2,458
Loc: north kakalacky
Last seen: 7 months, 11 days
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Re: lab sterilization [Re: NOS4A2]
    #19135811 - 11/14/13 11:34 AM (10 years, 3 months ago)

Quote:

NOS4A2 said:
I've been outa the scene for awhile so bear with me, but I'm a little shocked at the negative points given to GB's over a SAB. Why am I not seeing what's so much better about a box with holes and hands pumping in and out compared to a sealed box which is actually more of a SAB than a flexible holy rubbermaid and dish washing gloves? Please fill me in.



One word for the setup you describe above.  AWKWARD!  Try it the other way you will see.  it isn't sterile or completely still either way.  And being able to put things in and out without having to take the lid on and off makes a huge difference in practicality.


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Registered: 04/08/12
Posts: 22,502
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Re: lab sterilization [Re: NOS4A2]
    #19135920 - 11/14/13 12:02 PM (10 years, 3 months ago)

Quote:

NOS4A2 said:
When you first use a GB you find out real quick that the result of having a conventional alcohol lamp constantly burning in a GB can be a very sketchy proposition.



Especially if you use a flammable substance like alcohol or lysol inside....then it's just idiotic....but I get what you mean.


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InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
Re: lab sterilization [Re: PussyFart]
    #19139735 - 11/15/13 05:24 AM (10 years, 3 months ago)

yes with a GB you definitely gotta plan ahead. Perhaps it's better for a short sequence of events like innoc'ing some plates. Doing a bunch of quart G2G jars would definitely be a PITA.

Thank you for sharing your point of view. It's opened my mind up to some new ways things can be improved.


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OfflineeLeSDenes
Mycelium Expander
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Folding@home Statistics
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Re: lab sterilization [Re: PussyFart]
    #19142529 - 11/15/13 08:09 PM (10 years, 3 months ago)

Quote:

Notahacker420 said:
Quote:

NOS4A2 said:
When you first use a GB you find out real quick that the result of having a conventional alcohol lamp constantly burning in a GB can be a very sketchy proposition.



Especially if you use a flammable substance like alcohol or lysol inside....then it's just idiotic....but I get what you mean.




:grin: long time ago i was working in a SAB doing some agar shit, sprayed everything down with alcohol and then did not really think about that i should not flame sterilize my tools, my still air box almost got exploded. i was shaking the box like hell to extinguish the fire. All my agar plates and the fire made a mess:D i had extrathick gloves on but i was panicking that wtf is happening:D
so guys... don't spray alcohol everywhere if you are going to flame sterilize... or just wait a little bit until it dries up.


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OfflineOICU812
NC Tree Farm owner
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Registered: 11/06/11
Posts: 1,064
Loc: Foothills of NC Flag
Last seen: 3 years, 5 months
Re: lab sterilization [Re: eLeSDenes]
    #19150483 - 11/17/13 04:08 PM (10 years, 3 months ago)

I dug-up an interesting article from Fungi Magazine Fall 2011 by Rebecca Miller who has worked in the spawnmaking industry for many years in Western Pennsylvania.  Notice that she has just cut a rectangular hole for her hands to fit through, simple but effective according to her. 

I also noticed that her hands and arms are un-gloved in the picture.  I would like to know if this was purely for demonstration of if she advocates same.  I may try to contact her to ask.

I do not agree, however, with the flame inside the box, but would encourage builders to cut a small (3/4-1") hole in the side of the box through which a flame immediately adjacent to the box can be accessed.




--------------------
--------------
"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

"Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin
----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson


Edited by OICU812 (11/17/13 04:49 PM)


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OfflinePussyFart
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Registered: 04/08/12
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Re: lab sterilization [Re: OICU812]
    #19151736 - 11/17/13 08:47 PM (10 years, 3 months ago)

Quote:

OICU812 said:
I dug-up an interesting article from Fungi Magazine Fall 2011 by Rebecca Miller who has worked in the spawnmaking industry for many years in Western Pennsylvania.  Notice that she has just cut a rectangular hole for her hands to fit through, simple but effective according to her. 

I also noticed that her hands and arms are un-gloved in the picture.  I would like to know if this was purely for demonstration of if she advocates same.  I may try to contact her to ask.



Well she also has a live flame in the box....which totally defeats the purpose of the still air box, considering the heat currents.......

I think that is just for demonstration.....

Quote:

OICU812 said:
I do not agree, however, with the flame inside the box



Told ya.....lol.


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Offlineadastra80
Boss

Registered: 10/23/13
Posts: 50
Last seen: 10 years, 2 months
Re: lab sterilization [Re: PussyFart]
    #19156639 - 11/18/13 08:23 PM (10 years, 3 months ago)

Notahacker420, you're the real deal.


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OfflineTerry M
Stranger in a Strange Land
Male


Registered: 06/18/10
Posts: 1,502
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Re: lab sterilization [Re: adastra80]
    #19160302 - 11/19/13 03:47 PM (10 years, 3 months ago)

I seem to remember that Stamets uses ungloved hand. He doesn't like the feel of gloves. He simply washes his hands in alcohol very frequently.


--------------------
Liberté, égalité, humidité.


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OfflineOICU812
NC Tree Farm owner
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Registered: 11/06/11
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Re: lab sterilization [Re: Terry M]
    #19160866 - 11/19/13 05:44 PM (10 years, 3 months ago)

Quote:

Terry M said:
I seem to remember that Stamets uses ungloved hand. He doesn't like the feel of gloves. He simply washes his hands in alcohol very frequently.




Another good reason NOT to have a flame in the box!


--------------------
--------------
"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

"Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin
----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson


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OfflinePussyFart
Retired Cultivation Extrodinaire
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Re: lab sterilization [Re: OICU812]
    #19160948 - 11/19/13 05:59 PM (10 years, 3 months ago)

Bare hands mean lots of shedding dead skin cells.

Im str888.......


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