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Offlinebobsters
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Fool proof % 99 contam free glovebox? good idea?
    #19098919 - 11/07/13 03:28 AM (10 years, 3 months ago)

Hello, first time poster, year long lurker. Heres the idea:construct sealed glove box. Left side of gb cut hole and seal in synthetic filter disc. Right side hook up hepa filter (all sealed of course).

Before using insert all tools/specimens/cleaning supplies, clean w/bleach solution and then run hepta filter for 30 min. Shouldnt this rule out contams %99 of the time!

Only reason I'm asking is because I grew lions mane inside controlled enviroment last year with only %33 success rate that gradually got worse cuz I was a noob and exposed my house to green mold several times by opening ruined mono's. So ya, have to be extra sterile than most.

Thanks for your time reading this post!



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OfflinePsilosopherr
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: bobsters]
    #19098968 - 11/07/13 04:07 AM (10 years, 3 months ago)

that sounds like a plastic ghetto flowhood dude. :lolwut:


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OfflineRogerRabbitM
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Re: Fool proof % 99 contam free glovebox? good idea? (moved) [Re: bobsters] * 1
    #19098974 - 11/07/13 04:14 AM (10 years, 3 months ago)

This thread was moved from Advanced Mycology.

Reason:
Not an advanced mycology topic.  Check the search engine for all the thousands of times this exact idea has been proposed by new growers.
RR


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OfflinePussyFart
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: bobsters]
    #19099580 - 11/07/13 09:49 AM (10 years, 3 months ago)

Quote:

bobsters said:
Hello, first time poster, year long lurker. Heres the idea:construct sealed glove box. Left side of gb cut hole and seal in synthetic filter disc. Right side hook up hepa filter (all sealed of course).



This has fail written all over it.

Filters hooked up to it makes it NOT sealed.

You want a SAB(Still Air Box) for this hobby, not a GB(Glove Box).

If you do not have a Laminar Flow Hood, still air is the next best thing.


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OfflinePussyFart
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: PussyFart]
    #19099584 - 11/07/13 09:51 AM (10 years, 3 months ago)

   


A tub with 2 arm holes....that is all you need.


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InvisiblebodhisattaMDiscordReddit
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: PussyFart]
    #19099681 - 11/07/13 10:23 AM (10 years, 3 months ago)

Quote:

Notahacker420 said:
   


A tub with 2 arm holes....that is all you need.




Nah man the SGFC and SAB are way to simple to work :lmafo:

I just got done putting a new flux capacitor on mine.


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OfflinePussyFart
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: bodhisatta]
    #19099687 - 11/07/13 10:24 AM (10 years, 3 months ago)

How many Jigawatts does that baby pump out when you hit 88mph?


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InvisiblebodhisattaMDiscordReddit
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: PussyFart]
    #19099698 - 11/07/13 10:26 AM (10 years, 3 months ago)

A little over 9000.

I think all the ionizing radiation is why I'm getting 0 contams.


Edited by Trusted cuItivator (11/07/13 10:29 AM)


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Invisibleelasticaltiger
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: PussyFart]
    #19099788 - 11/07/13 10:43 AM (10 years, 3 months ago)

Quote:

Notahacker420 said:
   


A tub with 2 arm holes....that is all you need.



Is that your SAB Notahacker? I have one about that size I was thinking of pitching because I was worried it would be too short for G2G.  Have you had any problems with a ceiling that low?


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflinePussyFart
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: elasticaltiger]
    #19099837 - 11/07/13 10:57 AM (10 years, 3 months ago)

It's a 110qt Sterlite tub, and yes, at first, I had problems with the low clearance for G2G.

But eventually, you learn to just fling your wrist a little(or at least I did), and instead of pouring the grains in, they kinda get shot into the receiving jar....I should make a video....lol....it's cool how it works.....sometimes it helps to tilt the receiving jar a little....

All in all I am very satisfied with the tub....even though it is shorter than most, it still works, and I have an uber amount of space.


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Offlinebobsters
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: PussyFart]
    #19103961 - 11/08/13 03:48 AM (10 years, 3 months ago)

Is that pvc pipe for the arm holes? I like how you seal the sab to let your air settle


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Invisibleelasticaltiger
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: bobsters]
    #19104213 - 11/08/13 07:16 AM (10 years, 3 months ago)

Quote:

bobsters said:
Is that pvc pipe for the arm holes? I like how you seal the sab to let your air settle




It is.  They are called 'clean out adapters' with 'plugs.'  If you have really fat forearms like me you might need to upgrade to the 6" version. The 4" ones just don't give me enough room to move.


--------------------
First time growing cakes? DON'T make a Shotgun Fruiting Chamber

The Shmuvbox. - The Old TC's Like it :shrug:

Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger!
No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit.

"The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT

Pastywhite's Easy Agar Tek (PastyPlates)

Tiger Drop Video Demos By munchauzen

Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson

EZEKIEL 23:20


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OfflineCaptainPuffy
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: elasticaltiger]
    #19104304 - 11/08/13 08:13 AM (10 years, 3 months ago)

What do you guys use inside the SAB? Bleach? Soap and Water?

I've been using an inverted tub with a bleached towel for the base; however, my old lady has a horrible allergy to bleach, and I've been trying to think of an alternate.


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InvisiblebodhisattaMDiscordReddit
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: CaptainPuffy]
    #19104310 - 11/08/13 08:16 AM (10 years, 3 months ago)

Soap and water. The contams are not gone in a SAB the air is just still so the contams don't get in your work. I only use alcohol to wipe the tops of the jars when I do a g2g transfer. Otherwise warm soapy water in a spray bottle spray it down and let it sit for 10 or so minutes.


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Offlinesytar
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: bodhisatta]
    #19104327 - 11/08/13 08:24 AM (10 years, 3 months ago)

Soap and water isn't much more effective than just spraying it with water if you don't apply friction, from what I understand.


--------------------
I post from my phone. Excuse the typos and autocorrects.


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InvisiblebodhisattaMDiscordReddit
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: sytar]
    #19104338 - 11/08/13 08:27 AM (10 years, 3 months ago)

It just coats the surface better rather than how water would just be beading up. I like the contams to stick to the wet surfaces and then stay there. I believe it's a surface tension thing rather than actually cleaning.


Edited by Trusted cuItivator (11/08/13 08:27 AM)


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Invisiblebootster
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: bodhisatta]
    #19104584 - 11/08/13 09:38 AM (10 years, 3 months ago)

Soap acts like a "wetting agent". That's one of the reasons straw is soaked in soapy water before spawning.


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Offlinewestthebest
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: bobsters]
    #19105239 - 11/08/13 11:47 AM (10 years, 3 months ago)

Quote:

bobsters said:
I like how you seal the sab to let your air settle




smart.  might start doing that...


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InvisiblebodhisattaMDiscordReddit
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: westthebest]
    #19105896 - 11/08/13 02:11 PM (10 years, 3 months ago)

Quote:

westthebest said:
Quote:

bobsters said:
I like how you seal the sab to let your air settle




smart.  might start doing that...




You always should. It's part of the operation of the SAB. You can use cleanouts, tyvek sleeves, paper towels or anything really.


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OfflineOICU812
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Re: Fool proof % 99 contam free glovebox? good idea? [Re: CaptainPuffy]
    #19133154 - 11/13/13 08:18 PM (10 years, 3 months ago)

Quote:

CaptainPuffy said:
What do you guys use inside the SAB? Bleach? Soap and Water?

I've been using an inverted tub with a bleached towel for the base; however, my old lady has a horrible allergy to bleach, and I've been trying to think of an alternate.




I use Star-San a lot as my second/alternative sanitizer.  It's used in home brewing to sanitize equipment and brewing vessels.  The $7.99 size linked here will make 40 gallons!



--------------------
--------------
"Democracy is two wolves and a lamb voting on what to have for lunch. Liberty is a well-armed lamb contesting the vote!" --Benjamin Franklin

"Those who give up liberty for security won't have, or deserve, either.". . . Benjamin Franklin
----> Read: The Fight of our Lives - Defeating the Ideological War Against the West - by Victor Davis Hanson


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