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Offlinethedude0345
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How many jars per syringe?
    #19132583 - 11/13/13 06:41 PM (10 years, 2 months ago)

hey guys, so i should be getting my spores in the mail tomorrow (thursday) and plan to start everything up then. Im doing just the basic PF tek BRF cake method and have 24 jars. I know most places say 1cc per jar, and with 2 10 cc syringes that would give me 20 jars. My question is though, can i stretch those 20cc's into 24 jars? I know some places say alot of spore syringes come with more than 10cc's in them so if thats the case then i should be fine.

Also, i have an alcohol lamp for flame sterilization and plan on using a home made glove box from a plastic bin with arm holes cut in it. Im under the impression that i need to flame sterilzie between jars but i feel like keeping the lamp inside the enclosed box isnt good for various reasons, and i also feel like removing and re entering my hands/the needle 24 times may render the glove box useless as i feel it would sort of bring in outside air.

Any help or advice would be appreciated!! :mushroom2:


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Offlinebrianstequila
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Re: How many jars per syringe? [Re: thedude0345]
    #19132627 - 11/13/13 06:51 PM (10 years, 2 months ago)

You dont need much in the jars, me personally, I would stretch all 24 with 1 syringe and have some left for an agar plate.


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Every citizen should be a soldier. This was the case with the Greeks and Romans, and must be that of every free state.
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:drunkdriver:http://www.shroomery.org/forums/showflat.php/Number/19161913:drunkdriver:


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Offlinethedude0345
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Re: How many jars per syringe? [Re: brianstequila]
    #19132649 - 11/13/13 06:56 PM (10 years, 2 months ago)

nice, i think im just going to use both spores for the jars then. ive been looking into doing bulk grows and other things but ive decided that i want to get at least one solid PF tek grow under my belt before i move on to bigger and better things


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Invisibleelasticaltiger
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Re: How many jars per syringe? [Re: brianstequila]
    #19132692 - 11/13/13 07:03 PM (10 years, 2 months ago)

Quote:

My question is though, can i stretch those 20cc's into 24 jars?




Yes. Definitely.  You can stretch them pretty darn far I think assuming you've shaken it well.  I remember reading a thread where a TC said to use a few drops only.  I think the 1 cc rule is used because it's hard to accurately measure anything less than 1/4 a cc by eyeball. Not that it can't be done, it just takes a degree of control and a little practice.  1/4 cc is pretty easy for most people to eyeball relatively accurately.


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OfflinePussyFart
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Re: How many jars per syringe? [Re: elasticaltiger] * 1
    #19132711 - 11/13/13 07:05 PM (10 years, 2 months ago)

You only need a drop on agar to get growth, so only a few drops per jar per hole is actually needed.

You can use 9cc of spores, then suck up 9cc of sterile water, and keep using the syringe.

Spores are very, very potent.


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Offlinebrianstequila
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Re: How many jars per syringe? [Re: PussyFart]
    #19132747 - 11/13/13 07:10 PM (10 years, 2 months ago)

:whathesaid:He is the one that got me to be stingy with the syringe, I have filled mine back up several times before with good results.


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Every citizen should be a soldier. This was the case with the Greeks and Romans, and must be that of every free state.
Thomas Jefferson

Not My trade list i aint got shit anymore
:drunkdriver:http://www.shroomery.org/forums/showflat.php/Number/19161913:drunkdriver:


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Offlinethedude0345
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Re: How many jars per syringe? [Re: PussyFart]
    #19132774 - 11/13/13 07:13 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
You only need a drop on agar to get growth, so only a few drops per jar per hole is actually needed.

You can use 9cc of spores, then suck up 9cc of sterile water, and keep using the syringe.

Spores are very, very potent.





wow, didnt know that but that is awesome. any advice on the flame sterilization? does the alcohol lamp go inside the glove box or am i okay to keep it out of the box but still sterilize between every jar?


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Offlinebrianstequila
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Re: How many jars per syringe? [Re: thedude0345]
    #19132807 - 11/13/13 07:18 PM (10 years, 2 months ago)

Keep it out expecially if you wipe it down with iso first, you dont want a bomb


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Every citizen should be a soldier. This was the case with the Greeks and Romans, and must be that of every free state.
Thomas Jefferson

Not My trade list i aint got shit anymore
:drunkdriver:http://www.shroomery.org/forums/showflat.php/Number/19161913:drunkdriver:


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Offlinethedude0345
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Re: How many jars per syringe? [Re: brianstequila]
    #19132817 - 11/13/13 07:20 PM (10 years, 2 months ago)

Quote:

brianstequila said:
Keep it out expecially if you wipe it down with iso first, you dont want a bomb




:thumbup: no i certainly dont want a bomb. just scared taking my hands in and out of the glove box 24 times might endanger sterility!


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OfflinePussyFart
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Re: How many jars per syringe? [Re: thedude0345]
    #19132831 - 11/13/13 07:22 PM (10 years, 2 months ago)

Quote:

thedude0345 said:
Quote:

brianstequila said:
Keep it out expecially if you wipe it down with iso first, you dont want a bomb




:thumbup: no i certainly dont want a bomb. just scared taking my hands in and out of the glove box 24 times might endanger sterility!



wait a minute for the air to settle each time before going back to work.


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OfflineGymspawn
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Re: How many jars per syringe? [Re: PussyFart]
    #19132922 - 11/13/13 07:36 PM (10 years, 2 months ago)

I never knew to do that Hacker thanks for the info. I got a question for hacker that might help the op as well if you don't mind?

I've heard that sucking air into the syringe can contam the whole syringe.
So the last time I sucked air into the syringe to properly shake and distribute the spores through the fire coming from flame sterilizing with my lighter.
This was kind of hard to get it just right so I ended up with some clumps of spores
so what are some other ways of properly distributing spores without sucking in the flame?


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Invisibledionysiandame
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Re: How many jars per syringe? [Re: brianstequila]
    #19132957 - 11/13/13 07:42 PM (10 years, 2 months ago)

Quote:

brianstequila said:
You dont need much in the jars, me personally, I would stretch all 24 with 1 syringe and have some left for an agar plate.




See this is good to know, as I know I used 1 syringe to make about 6 jars and that was probably way too much. Now I know to stretch that out because the mycellia will kick off regardless.


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Offlinethedude0345
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Re: How many jars per syringe? [Re: Gymspawn]
    #19133034 - 11/13/13 07:55 PM (10 years, 2 months ago)

Quote:

Gymspawn said:
I never knew to do that Hacker thanks for the info. I got a question for hacker that might help the op as well if you don't mind?

I've heard that sucking air into the syringe can contam the whole syringe.
So the last time I sucked air into the syringe to properly shake and distribute the spores through the fire coming from flame sterilizing with my lighter.
This was kind of hard to get it just right so I ended up with some clumps of spores
so what are some other ways of properly distributing spores without sucking in the flame?




i was wondering this as well but i plan on just shaking the shit out of the syringe. sucking outside air into the syringe sounds risky to me


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Offlineclueless
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Re: How many jars per syringe? [Re: thedude0345]
    #19133214 - 11/13/13 08:31 PM (10 years, 2 months ago)

No need to suck air in just shake like crazy. :crazy2:


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