Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Kratom Capsules for Sale   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck

Jump to first unread post Pages: < Back | 1 | 2 | 3  [ show all ]
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Never buy spores again? [Re: krypto2000]
    #19130372 - 11/13/13 08:52 AM (10 years, 3 months ago)

buy syringe

make sporeprints from the edible mushrooms you grow
make liquid culture from the shrooms you grow(from agar isolation) , enough culture for eternity

each sporeprint is enough for several syringes...

LC or sporeprints can be stored for years
sporeprints can be stored for eternity (just place the cap of a mushroom on alufoil, let dry,store it)

if you do agar you only need 1 or 2 spores? to germinate, I forgot how it works that lowlevel ;-)
which means 1 sporeprint can give you xxx isolations :-)

1 spore print is enough for eternity, if you do agar/LC

syringes have a limited lifetime though iirc... you better use the syringe you made
make LC from it, do agar from it, grow from it


Extras: Filter Print Post Top
InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
 User Gallery

Folding@home Statistics
Registered: 04/30/13
Posts: 61,890
Loc: Milky way
Trusted Cultivator
Re: Never buy spores again? [Re: krypto2000]
    #19130730 - 11/13/13 11:00 AM (10 years, 3 months ago)

Quote:

krypto2000 said:
Also if you're just growing a few mono's or less then a LC or agar are both a waste of time imo, typically you should only do agar or LC's if you plan to grow a lot or over a long period of time which is why I don't think it's significant that you'll have to wait an additional 2 weeks or so for it to grow and be tested.

As far as LC's vs Agar though I just don't think they're very comparable. Agar is for isolation and LC is for bulk inoculation. Whether you start your LC from agar or ms is irrelevant. Either way you shouldn't start your LC from an unknown source and expect it to be clean, however if you do start it from a clean source there's likewise no reason you should expect it to be dirty.




:lolwut:
I don't even grow a single full sized monotub and agar is my most valuable tool. I'm not looking for yields but I'm looking for quality and clean viable cultures. There's really no time involved. It's all in a pipeline anyway. After the first 2 weeks there's no waiting there's always work.

You think a MS syringe is ever clean?? Unless you're growing in-vitro fruits and then opening the jar in front of a flow hood or in a SAB and then taking a print and then putting that straight to a LC you're not using a clean spore print.

If you start a LC with a needle you should expect it to be dirty.

Inoculation in general(MS or LC) sucks ass for bulk, Period. mycellium expansion is where it's at ala G2G.

Who in their right mind growing bulk would want to inoculate 100 jars with a less-trusty needle....


Edited by Trusted cuItivator (11/13/13 11:01 AM)


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Never buy spores again? [Re: bodhisatta]
    #19130751 - 11/13/13 11:08 AM (10 years, 3 months ago)

:whathesaid:


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
OfflineChilled
Out


Registered: 04/21/12
Posts: 261
Loc: Flag
Last seen: 4 years, 9 months
Re: Never buy spores again? [Re: SpitballJedi]
    #19130947 - 11/13/13 12:07 PM (10 years, 3 months ago)

Not disagreeing with what has been said here (been an interesting read) but could you not use MS syringe to get started with WBS jars (in a SAB as good practise IMO) or other grain jar, wait for colonisation, then grain LC some jars and other jars G2G using a SAB and good sterile procedure (just to try both methods to see which I prefer). Then use a bit of colonised grain for Agar work.

I ask this as tried Agar with both MS syringe and a spore print and both were fiddly and not successful, plus I cant see how either would be a very sterile starting point. Clearly it can be done but since you can still get a isolate but starting with something slightly more contam resistant like a bit of grain, would this not be easier?

Love to hear peoples thoughts on this...


Extras: Filter Print Post Top
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Never buy spores again? [Re: Chilled]
    #19130964 - 11/13/13 12:11 PM (10 years, 3 months ago)

There is only one way to be clean... agar

but sometimes you get clean syringes.... if you use a reputable vendor

sometimes there can be bacteria in though...

agar solves it all, and you don't need a flowhood for success
still air box

then you can do agar->LC

Never do biopsy -> LC or syringe->LC , if you want to be successful first time
same goes for sporeprint ->syringe .. no go.... assume any sporeprint to be contamed

might work first 2 times, and fail on the 3th
if you do agar you will get close to 100% success which is much better


Extras: Filter Print Post Top
OfflinecronicrFacebook
 User Gallery


Registered: 08/07/11
Posts: 61,436
Loc: Van Isle Flag
Last seen: 2 years, 1 month
Trusted Cultivator
Re: Never buy spores again? [Re: Chilled] * 1
    #19130985 - 11/13/13 12:14 PM (10 years, 3 months ago)

Quote:

Chilled said:
Not disagreeing with what has been said here (been an interesting read) but could you not use MS syringe to get started with WBS jars (in a SAB as good practise IMO) or other grain jar, wait for colonisation, then grain LC some jars and other jars G2G using a SAB and good sterile procedure (just to try both methods to see which I prefer). Then use a bit of colonised grain for Agar work.





you sure can

Quote:

Chilled said:

I ask this as tried Agar with both MS syringe and a spore print and both were fiddly and not successful, plus I cant see how either would be a very sterile starting point. Clearly it can be done but since you can still get a isolate but starting with something slightly more contam resistant like a bit of grain, would this not be easier?

Love to hear peoples thoughts on this...



colonized grain works fine, it takes off quickly on agar(because of the lower nutrition level in the agar)


--------------------

It doesn't matter what i think of you...all that matters is clean spawn

I'm tired do me a favor


Extras: Filter Print Post Top
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Never buy spores again? [Re: bodhisatta]
    #19132439 - 11/13/13 06:06 PM (10 years, 3 months ago)

Agar is your best bet

and is easy... once you buy agar agar at your local supermarket/health food store
it is a gelatine substitute from seaweed, for vegans

agar is the only way to get growing reliably
do it right from start, because before you know you will get contam in everything
just do agar from start, save yourself some time

a still air box will make your innoculations more successful
and you use that for agar

if you don't do agar... you can keep buying syringes as about the only option
else I wouldn't count on reliable growing

search for "oatmeal agar" tek, can recommend that one, works -very- good, mycellium loves it
no need for PDA, oatmeal you usually got at home

edit:
fixed the misunderstanding, thx for mentioning :-)


Edited by lessismore (11/13/13 06:16 PM)


Extras: Filter Print Post Top
OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 10 days
Trusted Cultivator
Re: Never buy spores again? [Re: lessismore]
    #19132466 - 11/13/13 06:11 PM (10 years, 3 months ago)

Quote:

mio said:
a glovebox(still air box) will make your innoculations more successful and you use that for agar



A GB(Glove Box) is not the same thing as a SAB(Still Air Box).

A SAB is better for this hobby than a GB because the gloves are attached to you and not the box.

Making it possible to flame sterilize your tool outside the box between transfers.

Also when people try to "seal" their GBs, the spots that they missed(like around the lid) will constantly suck air in and out with your movements.

This will negate the "still air environment"....


Edited by PussyFart (11/13/13 06:12 PM)


Extras: Filter Print Post Top
Invisiblelessismore
Registered: 02/10/13
Posts: 6,268
Re: Never buy spores again? [Re: PussyFart]
    #19132474 - 11/13/13 06:13 PM (10 years, 3 months ago)

Yes, that is what I ment too
sorry if it was unclear, fixed it in edit now

still air box is what you want, any high bedroller tub with 2 holes in it so you can get your hands in, and a lid

for agar innoc/transfer: inoculation loop is what is used, or scalpel (innoc loop can be made from twisted wire + a pen)
alcohol lamp is a plus and gloves are a plus too, sterilize red hot outside, bring inside

agar will make your life much easier

oatmeal agar is some good stuff..... if you cannot find PDA, or MDA i.e.,  pda/mda might be easier to mix together initially, dunno

cubensis seems to love oatmeal agar to what I've seen, it's a pretty good tek, grows perfectly - perfect consistency

only downside with oatmeal agar is it can be bothersome to mix together IMO
PDA/MDA might be better there , might save time in preparation (no need to extract the juice from oatmeal)
benefit is it is very easy to find, PDA/MDA you might not have luck finding

agar is nutrient less, you need some nutrients for your agar, for mycellium to grow in your jars/petris
PDA = potato dextrose agar
MDA = malt dextrose agar
or oatmeal agar


Edited by lessismore (11/13/13 06:28 PM)


Extras: Filter Print Post Top
Offlinetwistedty
Forcefully Retired
Male User Gallery


Registered: 07/01/12
Posts: 5,487
Loc: Middle
Last seen: 3 years, 7 months
Re: Never buy spores again? [Re: bodhisatta]
    #19133205 - 11/13/13 08:27 PM (10 years, 3 months ago)

Quote:

bodhisatta said:



I don't even grow a single full sized monotub and agar is my most valuable tool. I'm not looking for yields but I'm looking for quality and clean viable cultures. There's really no time involved. It's all in a pipeline anyway. After the first 2 weeks there's no waiting there's always work.

You think a MS syringe is ever clean?? Unless you're growing in-vitro fruits and then opening the jar in front of a flow hood or in a SAB and then taking a print and then putting that straight to a LC you're not using a clean spore print.

If you start a LC with a needle you should expect it to be dirty.

Inoculation in general(MS or LC) sucks ass for bulk, Period. mycellium expansion is where it's at ala G2G.

Who in their right mind growing bulk would want to inoculate 100 jars with a less-trusty needle....





:whathesaid:


agar is the best hands down no argument.


Extras: Filter Print Post Top
Jump to top Pages: < Back | 1 | 2 | 3  [ show all ]

Shop: PhytoExtractum Kratom Powder for Sale   North Spore Bulk Substrate   Original Sensible Seeds Bulk Cannabis Seeds   Bridgetown Botanicals CBD Concentrates   Myyco.com Isolated Cubensis Liquid Culture For Sale   Kraken Kratom Kratom Capsules for Sale   Left Coast Kratom Buy Kratom Capsules   Mushroom-Hut Liquid Cultures   MagicBag.co All-In-One Bags That Don't Suck


Similar ThreadsPosterViewsRepliesLast post
* Can spores be directly injected into potting soil? LSAuser 9,319 10 09/10/02 12:16 PM
by SixCee
* Spore Bacterial Infection?
( 1 2 all )
BladeLSD 12,159 34 05/21/03 03:30 PM
by Suntzu
* Spore syringe question... zoomzoom 1,531 4 11/21/02 09:31 PM
by user_exists
* Spore Mixing goober420 629 2 12/27/01 12:34 AM
by isis
* wasted spores Blu Spore 2,994 3 02/22/19 10:59 AM
by LosTresOjos
* Re: Spore Price Comparison spores 5,048 16 07/27/00 12:46 PM
by Mycelium5150
* Temp at which SPORES die (in syringe form) not mycelium ModestMouserrrrr 5,879 15 02/07/10 01:03 PM
by RogerRabbit
* Did my pet lemming inject too much!? DarkLemming 1,098 5 10/08/02 09:20 PM
by unsecreto

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
4,439 topic views. 29 members, 167 guests and 21 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.029 seconds spending 0.008 seconds on 13 queries.