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InvisibleKavalo
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Registered: 04/09/00
Posts: 39
Loc: Portugal
Any tips on how to make a LC syringe from a cake?
    #19129013 - 11/12/13 10:33 PM (10 years, 3 months ago)

Greetings,

Here's the problem:
I need to make a LC syringe and only have 5 almost fully colonized PF cakes made in a drinking glass with tinfoil as a lid.
Because I used the tinfoil lids I presume it is not adequate to try the Poor Mans Syringe Tek ( http://www.shroomery.org/67/Poor-Mans-Mycelium-Syringe-Tek ).

I was wondering if it was possible to:
1) dunk a cake with a little bit of bleach to avoid possible contaminants and then put in in a ziplock, crumble it, and inject water with a syringe to create a LC;
2) dunk it with bleach, crumble it, and use a piece of it to start a grain culture an then (if it works out) try a GLC.
(All working in a SAB).

I know these aren't the best choices, but I really need to make a LC.

Please share some of your thoughts and/or suggest alternative techniques.

Thanks in advance,
K.


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OfflinecronicrFacebook
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Re: Any tips on how to make a LC syringe from a cake? [Re: Kavalo]
    #19129022 - 11/12/13 10:35 PM (10 years, 3 months ago)

y do you need a lc? your verm barrier could be harbouring lots of contams, not  a great idea


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InvisibleFrankHorrigan
The Inquisition
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Registered: 01/04/11
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Re: Any tips on how to make a LC syringe from a cake? [Re: cronicr]
    #19129046 - 11/12/13 10:39 PM (10 years, 3 months ago)

Here's how to make an LC from your cake:

1) Grow mushrooms.

2) Take spore prints.

3) Use these spores to inoculate your LC.

4) Inoculate more LCs with spores because they are bound to contam quite often.

5) Test LCs.

6) Use clean LCs, throw away bad ones.


--------------------

Yes, you can bump my old threads with a question.
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You should take a look. :hehehe:


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AMU- Get an answer here -AMU


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InvisibleKavalo
Phallic Mushroom

Registered: 04/09/00
Posts: 39
Loc: Portugal
Re: Any tips on how to make a LC syringe from a cake? [Re: FrankHorrigan]
    #19129095 - 11/12/13 10:49 PM (10 years, 3 months ago)

Frank,

I know that is by far the best choice. But if you were to make a liquid culture with a cake (without waiting for the spores) what do you suggest to be the best (of the worst...) approach?


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OfflinePussyFart
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Re: Any tips on how to make a LC syringe from a cake? [Re: Kavalo]
    #19129106 - 11/12/13 10:50 PM (10 years, 3 months ago)

Quote:

Kavalo said:
I know that is by far the best choice. But if you were to make a liquid culture with a cake (without waiting for the spores) what do you suggest to be the best (of the worst...) approach?



Wait for spores.....


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OfflinecronicrFacebook
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Re: Any tips on how to make a LC syringe from a cake? [Re: PussyFart]
    #19129113 - 11/12/13 10:50 PM (10 years, 3 months ago)

:whathesaid:


--------------------

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Offlinetwistedty
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Re: Any tips on how to make a LC syringe from a cake? [Re: cronicr]
    #19129949 - 11/13/13 03:52 AM (10 years, 3 months ago)

agar


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OfflineJMcDoogle
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Re: Any tips on how to make a LC syringe from a cake? [Re: FrankHorrigan]
    #19130144 - 11/13/13 06:24 AM (10 years, 3 months ago)

Quote:

FrankHorrigan said:
Here's how to make an LC from your cake:

1) Grow mushrooms.

2) Take spore prints.

3) Use these spores to inoculate your LC.

4) Inoculate more LCs with spores because they are bound to contam quite often.

5) Test LCs.

6) Use clean LCs, throw away bad ones.




Or

Scratch all that

Do Agar to WBS for purpose of
Grain 2 Grain


--------------------
The ego is nothing other than the focus of conscious attention.


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InvisibleSpitballJedi
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Re: Any tips on how to make a LC syringe from a cake? [Re: Kavalo]
    #19130198 - 11/13/13 07:01 AM (10 years, 3 months ago)

With tin foil for a lid, I'm honestly surprised your cake is not growing contaminants already. If you are using a dry vermiculite layer as your contam barrier, I'd say that is quite a testament to how affective it is.

Making LC is a risky endeavor as it is. Making one from a cake has a high likelihood of fail.

It will be very difficult to keep contams out of your rich LC. Once they get in there, it's likely to be a party for them.

Like everyone said, you really should get a spore print or make agar.


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Edited by SpitballJedi (11/13/13 10:42 AM)


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OfflineJMcDoogle
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Re: Any tips on how to make a LC syringe from a cake? [Re: SpitballJedi]
    #19130226 - 11/13/13 07:21 AM (10 years, 3 months ago)

The term I think you're looking for is
GLC
Grain to Liquid Culture.

My rudimentary understanding is this:

You would inject a sterile liquid into your

(preferably) GRAIN colonized growth medium

swirl it around, than reclaim that liquid for

use to inject into more growth mediums.


--------------------
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InvisibleFrankHorrigan
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Re: Any tips on how to make a LC syringe from a cake? [Re: JMcDoogle]
    #19130568 - 11/13/13 10:04 AM (10 years, 3 months ago)

Quote:

JMcDoogle said:
Or

Scratch all that

Do Agar to WBS for purpose of
Grain 2 Grain




Well yes but I am assuming the OP is a new cultivator who doesn't want to invest in a whole new set of 'stuff' for his grow.


--------------------

Yes, you can bump my old threads with a question.
Here is how I get things done.
You should take a look. :hehehe:


Frank's tips and tricks. Updated on 3/21/14
AMU- Get an answer here -AMU


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InvisiblebodhisattaMDiscordReddit
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Re: Any tips on how to make a LC syringe from a cake? [Re: Kavalo]
    #19130705 - 11/13/13 10:51 AM (10 years, 3 months ago)

Quote:

Kavalo said:
but I really need to make a LC.

Please share some of your thoughts and/or suggest alternative techniques.

Thanks in advance,
K.




No you really don't.

Say you don't want to get into agar yet. Take spore prints and make spore syringes. The time it will take for the LC to germinate + then turning that into a syringe and inoculating more cakes will take longer than just having used the spores in the first place.

You're adding a step even though it seems like your cakes will go faster with the LC.

I'm new at agar. Maybe a month of experience. I have gotten a bunch of little spots of contamination making petris from spores. I wouldn't want one of those little dots of contam making it into my LC and waste all of my time and effort. You're going to literally have to make 10+ of these things and try em out just to find the couple that are clean if you're starting from spores.


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OfflinecronicrFacebook
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Re: Any tips on how to make a LC syringe from a cake? [Re: bodhisatta]
    #19130759 - 11/13/13 11:10 AM (10 years, 3 months ago)

shit if i'm making my own prints/syringes they would be faster then making a lc:shrug:


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It doesn't matter what i think of you...all that matters is clean spawn

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OfflineWhiplash
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Registered: 09/20/13
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Last seen: 9 years, 2 months
Re: Any tips on how to make a LC syringe from a cake? [Re: bodhisatta]
    #19130769 - 11/13/13 11:12 AM (10 years, 3 months ago)

I am a total noob on a first grow and know nothing. Nothing I say should even be considered. However I like learning from mistakes and like to try things out.

One of these experiments I had I made a syringe with sterile water and just before I birthed one of my BRF Jars I took the syringe and stuck it far down the side of my jar, between the glass and the cake. I then shot 12mls of sterile water into the jar, removed needle and swirled it for about 2 minutes. I then flame sterilized the needle and stuck it back down the side. I drew about 6mls of water back up and pretty much used that immediatly to inoculate some jars.

Within a few days there was growth and they colonized just like the original jar. I have not birthed them yet as my FC is full but they are "waiting" in a fridge for birthing as soon as possible. I will only really know (probably not even then) if I succeeded once they are out. But they seem identical to the original jar.

Here is the inoculated jar


As far as using that for LC??? I cannot say. But I thought Id put that out there



Edit: Oops. I only saw now that you allready posted the poor mans syringe tek. Ignore me please


Edited by Whiplash (11/13/13 11:15 AM)


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InvisiblebodhisattaMDiscordReddit
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Re: Any tips on how to make a LC syringe from a cake? [Re: Whiplash]
    #19130827 - 11/13/13 11:31 AM (10 years, 3 months ago)

Quote:

Whiplash said:
I am a total noob on a first grow and know nothing. Nothing I say should even be considered. However I like learning from mistakes and like to try things out.

One of these experiments I had I made a syringe with sterile water and just before I birthed one of my BRF Jars I took the syringe and stuck it far down the side of my jar, between the glass and the cake. I then shot 12mls of sterile water into the jar, removed needle and swirled it for about 2 minutes. I then flame sterilized the needle and stuck it back down the side. I drew about 6mls of water back up and pretty much used that immediatly to inoculate some jars.

Within a few days there was growth and they colonized just like the original jar. I have not birthed them yet as my FC is full but they are "waiting" in a fridge for birthing as soon as possible. I will only really know (probably not even then) if I succeeded once they are out. But they seem identical to the original jar.

Here is the inoculated jar


As far as using that for LC??? I cannot say. But I thought Id put that out there



Edit: Oops. I only saw now that you allready posted the poor mans syringe tek. Ignore me please





Sometimes you get lucky sure.

This is like driving your car at night with the lights off. Does it work, yes technically, but it's not always going to end well.


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OfflineJMcDoogle
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Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
Last seen: 3 years, 10 months
Re: Any tips on how to make a LC syringe from a cake? [Re: FrankHorrigan]
    #19132786 - 11/13/13 07:15 PM (10 years, 3 months ago)

Quote:

FrankHorrigan said:
Quote:

JMcDoogle said:
Or

Scratch all that

Do Agar to WBS for purpose of
Grain 2 Grain




Well yes but I am assuming the OP is a new cultivator who doesn't want to invest in a whole new set of 'stuff' for his grow.




Exactly, you are correct.


--------------------
The ego is nothing other than the focus of conscious attention.


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OfflineKiya_Star427
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Registered: 10/14/13
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Re: Any tips on how to make a LC syringe from a cake? [Re: JMcDoogle]
    #19132934 - 11/13/13 07:38 PM (10 years, 3 months ago)

scratch off some clean mycelium, run it under water, PC your LC substrate, then stick it in there. No agar, or growing mushrooms/spore print needed


--------------------


Edited by Kiya_Star427 (11/13/13 07:39 PM)


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InvisibleSpitballJedi
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Re: Any tips on how to make a LC syringe from a cake? [Re: Kiya_Star427]
    #19132956 - 11/13/13 07:42 PM (10 years, 3 months ago)

Expect failure when using that advice.


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OfflineKiya_Star427
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Registered: 10/14/13
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Re: Any tips on how to make a LC syringe from a cake? [Re: SpitballJedi]
    #19132969 - 11/13/13 07:44 PM (10 years, 3 months ago)

expect failure most the time you use an LC, whats your point? You never know it could work..


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InvisibleSpitballJedi
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Re: Any tips on how to make a LC syringe from a cake? [Re: Kiya_Star427]
    #19132991 - 11/13/13 07:47 PM (10 years, 3 months ago)

Yeah, it could work, but I wouldn't expect it to. I put odds of success at less than 1%.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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