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TheApprentice
back at it



Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 2 months
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spores, no growth
#19125209 - 11/12/13 06:58 AM (10 years, 3 months ago) |
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I made 3 agar plates last week Monday and inoculated them with my new inoculation loop I made from a coat hanger.
I rubbed the loop on the spore print, then to the agar.
8 days later, 2 of my jars have contams at the edge (must have knocked them in). 3rd jar has no contam, but no myc either.
All 3 show no signs of spore germination.
wtf. The spores have been sitting for about a year 
did I do it wrong? Or should I just buy a new syringe.
I threw my print away because it has been used 2 times before
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RR Videos -Best $9 Ever Spent * No Pour AGAR Tek * Easy COIR Trays! * Pink Oysters on Newspaper TEK "Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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OregonMushys
Rye Wata Whippin



Registered: 09/09/13
Posts: 280
Last seen: 6 years, 4 months
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They usually take 7-10 days at least before you see anything. You're supposed to flame sterilize the loop, swipe it like 2 times in the agar plate to cool it, swipe spores onto loop, then swipe spores from loop onto center of agar. I did this with a wild stuntzii print like 19 days ago and havn't seen any growth either.

If youre using a spore syringe, flame needle, bring inside SAB let the very first drop cool needle and fall then let the second drop inoc the agar.
-------------------- Ps. Cubensis Ps. Cyanescens Ps. Stuntzii *GrowLog*
    
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TheApprentice
back at it



Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 2 months
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I did it right then...
maybe it was the spores. but I find it hard to believe no spores got attached to the loop...
I guess I'll just buy a new spore syringe.
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RR Videos -Best $9 Ever Spent * No Pour AGAR Tek * Easy COIR Trays! * Pink Oysters on Newspaper TEK "Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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Pestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
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There's no need to throw out a print because it's been used twice. A print can be used dozens of times. :-)
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   The Corbett Report Open Source Intelligence News
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rustycobwebs
Green mold cultivator

Registered: 12/17/05
Posts: 799
Last seen: 8 years, 10 months
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Re: spores, no growth [Re: Pestile]
#19125511 - 11/12/13 09:32 AM (10 years, 3 months ago) |
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Spores a year old might require some re hydration to germinate. Should have tried making it a syringe before throwing it I guess.
-------------------- and let it be so Praise Psilocybe
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Quote:
rustycobwebs said: Spores a year old might require some re hydration to germinate. Should have tried making it a syringe before throwing it I guess.
this! nice to see ya around apprentice!
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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TheApprentice
back at it



Registered: 09/25/11
Posts: 4,727
Last seen: 2 years, 2 months
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Re: spores, no growth [Re: cronicr]
#19127126 - 11/12/13 03:58 PM (10 years, 3 months ago) |
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Ya I've had some personal issues I've been dealing with.
all my mushrooms died in the process. Trying to get everything started again, and the mushroom gods are making me work 2x as hard.
ya, I got to thinking about rehydration since these spores didn't germinate. One of the plates I scraped the spores on, and they didn't go anywhere.
oh well, a lucky vendor will get my $12 next paycheck.
ya, how sad is it that having the skills I have...
only have $2 in the bank. LOL. Its time!
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RR Videos -Best $9 Ever Spent * No Pour AGAR Tek * Easy COIR Trays! * Pink Oysters on Newspaper TEK "Yeah? Well, DRACULA called... and he said he's coming over tonight, and I said OK!"
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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