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OfflineMasonic
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Found some lions mane.
    #19125524 - 11/12/13 09:39 AM (10 years, 2 months ago)

I found some lions mane growing wild. Can I take a tissue sample and star a culture on agar? Anyone have experience growing these indoors?


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OfflineForresterM
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Re: Found some lions mane. [Re: Masonic]
    #19125606 - 11/12/13 10:07 AM (10 years, 2 months ago)

You sure can.  They're pretty easy to grow.  Agar --> grain --> sawdust/fuel pellets.  Do a search for recent threads there's some good ones.


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
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Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinekushrooms
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Re: Found some lions mane. [Re: Forrester]
    #19128660 - 11/12/13 09:07 PM (10 years, 2 months ago)

Congrats!!! And what forester said 4 sure! :smile:


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"All are archetects of fate..."

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Offlineforrest
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Re: Found some lions mane. [Re: kushrooms]
    #19129440 - 11/12/13 11:56 PM (10 years, 2 months ago)

isn't it that also the inner more compact part of the lions mane are tubes compressed together, wich when they grew, closed in contaminants?

i never cloned them, but i remember reading something like that here, and the technike recommended was putting the tissue sample on agar, and then covering it with agar, with the idea that the fungus grows throug it faster than the contaminants.
or maybe use H2O2.

but can anyone verivy or dismiss this?


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OfflineMrcloudy
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Re: Found some lions mane. [Re: forrest]
    #19129784 - 11/13/13 02:16 AM (10 years, 2 months ago)

I found what I believe to be Herecium americanum. I cloned it to agar and it contamed pretty bad with bacteria which was expected. So i set it aside and checked on it yesterday to see a large mass of mycelium coming off from tissue sample. Once I had seen the bacteria I didn't expect too much. But it surprised me.  I'd say give it a try but expect to get bacteria.


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10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflineMolimo
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Re: Found some lions mane. [Re: Mrcloudy]
    #19131801 - 11/13/13 03:27 PM (10 years, 2 months ago)

I found some lion's mane last weekend that I'm also trying to clone. I did 6 cuttings last night, hopefully one of them won't contaminate.


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Invisiblebootster
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Re: Found some lions mane. [Re: Molimo]
    #19131858 - 11/13/13 03:40 PM (10 years, 2 months ago)

I've seen the myc do some impressive stuff on the Lions Mane. Sometimes it'll run right over some contaminants.


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OfflineMrcloudy
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Re: Found some lions mane. [Re: bootster]
    #19131936 - 11/13/13 03:58 PM (10 years, 2 months ago)

Quote:

bootster said:
I've seen the myc do some impressive stuff on the Lions Mane. Sometimes it'll run right over some contaminants.




How does this plate look? Its about 2 weeks old at this point. Initially the tissue sample I took was overrun by bacteria by day 3 so I set it aside. 2 weeks later I check it and see a huge wad of what looks like healthy mycelium with mycelium leading out across the plate. I have little experience with lions mane so I'm not even certain what the mycelium should look like.



--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflineSkysTheLimit
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Re: Found some lions mane. [Re: Mrcloudy]
    #19134845 - 11/14/13 05:40 AM (10 years, 2 months ago)

Hmm..shouldn't you have quickly transferred it to another clean agar plate asap? It isn't too late to do now, but there sure is alot of bacteria and what seems like a contaminating mold.
Good luck mrcloudy!


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Offlinekushrooms
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Re: Found some lions mane. [Re: SkysTheLimit]
    #19134925 - 11/14/13 06:53 AM (10 years, 2 months ago)

What this person said, I haven't isolated from a wild sample yet. I have isolated away from contam though, and I would make the transfer as soon as you see healthy mycelium and definitely as soon as you see contam on the plate. It should clean up.


--------------------
"All are archetects of fate..."

Check out oyster pics :smile: Click Here




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Offlinenanncee
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Re: Found some lions mane. [Re: kushrooms]
    #19135391 - 11/14/13 09:40 AM (10 years, 2 months ago)

I have never personally done a lions mane tissue sample, but on multiple other samples I have had to allow the mycellium to take over the contam then transfer it. Sometimes if you transfer immediatly as you see growth of the mycellium it transfer the contam and it grows faster than the mycellium.

Obviously it's not like that all the time, in fact i don't do that very often. But I have had to use it recently on both a A. Bitorqis and G. Lucidum. I went through two sleeves trying to clean up the G. Lucidum before I just let it hangout for a month then got it clean.


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Offlinenanncee
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Re: Found some lions mane. [Re: nanncee]
    #19135396 - 11/14/13 09:41 AM (10 years, 2 months ago)

oh one more thing, I personally have noticed lions mane to be a bit slow on agar compared to say a oyster or shiitake.


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I am a small scale farmer, come check out what we do. www.facebook.com/biocentricbros

Check out our Youtube videos. www.youtube.com/biocentricbros


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OfflineMrcloudy
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Re: Found some lions mane. [Re: nanncee]
    #19136167 - 11/14/13 01:19 PM (10 years, 2 months ago)

Well starting out the bacteria was the only thing alive on the plate so I assumed it was a lost cause so I set it aside. I tend to set my contaminants aside to see what develops. I didn't even know I had mycelium at all until I looked at it the other day. I plan on transferring it as soon as I pour some more plates probably later today. 

I had actually anticipated going back to the samples which are mostly dry and starting over which I don't think would have worked to well. So live mycelium is a step up from that at least.

Quote:

nanncee said:
but on multiple other samples I have had to allow the mycellium to take over the contam then transfer it. Sometimes if you transfer immediatly as you see growth of the mycellium it transfer the contam and it grows faster than the mycellium.

Obviously it's not like that all the time, in fact i don't do that very often. But I have had to use it recently on both a A. Bitorqis and G. Lucidum. I went through two sleeves trying to clean up the G. Lucidum before I just let it hangout for a month then got it clean.




I have noticed that in a few of the plates I have set aside it looks like the mycelium I was after eventually became the dominant organism. But I have not yet tried to transfer from one of those plates. This will actually be my first time transferring with this degree of contam.


--------------------

10 different Ganoderma species from across the USA

AMU

MrCloudys guide to North American GanodermaUpdated A rough guide to North American Ganoderma species, with an emphasis on the laccate species.


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OfflineForresterM
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Re: Found some lions mane. [Re: nanncee]
    #19151474 - 11/17/13 07:44 PM (10 years, 2 months ago)

Quote:

nanncee said:
I have never personally done a lions mane tissue sample, but on multiple other samples I have had to allow the mycellium to take over the contam then transfer it. Sometimes if you transfer immediatly as you see growth of the mycellium it transfer the contam and it grows faster than the mycellium.

Obviously it's not like that all the time, in fact i don't do that very often. But I have had to use it recently on both a A. Bitorqis and G. Lucidum. I went through two sleeves trying to clean up the G. Lucidum before I just let it hangout for a month then got it clean.




I think you've got a really good point there, I've just been figuring this out myself.  Had some plates of P. eryngii that got some green mold.  Didn't even wanna mess with that so I left them for a month or so.  Completely overgrew all the mold, so I was like hmmmm... I wonder...

Cut a wedge and transferred, after only one more transfer (which was probably due to my shitty SAB more than the original green mold) I think it's clean.  We'll see, but I think it just completely grew over all the mold and even entrapped the spores.  Would appear so anyhow :shrug:


--------------------
Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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OfflineMolimo
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Re: Found some lions mane. [Re: Forrester]
    #19181873 - 11/24/13 09:38 AM (10 years, 2 months ago)

Well my lion's mane cuttings did nothing :frown:. I had to keep the fruit I found wrapped in paper towels and unrefrigerated for 24 hours because I was camping. Do you think I killed it doing this? How long is a sample viable for cloning after removal from the tree?


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OfflineTerry M
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Re: Found some lions mane. [Re: Molimo]
    #19181950 - 11/24/13 10:06 AM (10 years, 2 months ago)

I do all my contaminant isolation in a plastic bin Glove Box/Still Air Box. This allows me to work in my regular lab, and not worry about spreading mold spores via the flow hood air stream.


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Liberté, égalité, humidité.


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