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OfflineChuckBerry
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Registered: 12/12/11
Posts: 31
Last seen: 9 years, 8 months
Went to inoculate jar and whole syringe is somehow sucked into it without me doing anything?
    #19124010 - 11/11/13 11:10 PM (10 years, 2 months ago)

Any guesses what could have happened. Ive done the pf tek a few times before without any problems but for what ever reason i go to inoculate my jars and poof! All my spores into one jar. What might happen to it? Anything i can do to make it more likely to survive?


Edited by ChuckBerry (11/11/13 11:11 PM)


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OfflineOgreLokon
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Registered: 06/30/13
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Re: Went to inoculate jar and whole syringe is somehow sucked into it without me doing anything? [Re: ChuckBerry]
    #19124030 - 11/11/13 11:16 PM (10 years, 2 months ago)

Sounds like your jars had negative pressure. Next time make sure your jar lids are slightly loose while sterilizing, and completely cool before inoculation.


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OfflineMaJiK_420
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Registered: 06/30/08
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Re: Went to inoculate jar and whole syringe is somehow sucked into it without me doing anything? [Re: OgreLokon]
    #19124060 - 11/11/13 11:23 PM (10 years, 2 months ago)

What kind of lids are you using? I don't know how that could happen if your following pftek with the 4 hole lid and verm barrier.


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OfflineChuckBerry
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Registered: 12/12/11
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Last seen: 9 years, 8 months
Re: Went to inoculate jar and whole syringe is somehow sucked into it without me doing anything? [Re: OgreLokon]
    #19124088 - 11/11/13 11:30 PM (10 years, 2 months ago)

Quote:

OgreLokon said:
Sounds like your jars had negative pressure. Next time make sure your jar lids are slightly loose while sterilizing, and completely cool before inoculation.



Damn that sounds about right.


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Offlinekneesocks
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Registered: 12/25/11
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Re: Went to inoculate jar and whole syringe is somehow sucked into it without me doing anything? [Re: ChuckBerry]
    #19124096 - 11/11/13 11:33 PM (10 years, 2 months ago)

Yes, either the jar had negative pressure or the syringe had positive pressure (which would be weird).


--------------------
"An ignorant man is lost, faithless, and filled with self-doubt;
A soul that harbors doubt has no joy, not in this world or the next."
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OfflinePsilicon
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Registered: 08/26/12
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Re: Went to inoculate jar and whole syringe is somehow sucked into it without me doing anything? [Re: kneesocks]
    #19124392 - 11/12/13 01:02 AM (10 years, 2 months ago)

The syringe didn't have positive pressure, because if it did it wouldn't have waited until he got it into the jar to squirt spores everywhere.

As someone already said, the PF tek works best with four holes for injection and GE.


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