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MaJiK_420
...lost



Registered: 06/30/08
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creeper tub turned PE?
#19123906 - 11/11/13 10:44 PM (10 years, 2 months ago) |
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I think this is second flush on some creepers. And then about half way through the flush I noticed a couple random PE mutants, but now everything on the sides are pretty much PE.
I already picked some of the regulars before I took the pictures, but I thought it was really weird. .. I got a batch of agar and I'm going to take some clone samples.
How often does something like this happen?
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19123957 - 11/11/13 10:56 PM (10 years, 2 months ago) |
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you don't tape your tubs? and that is pretty wild.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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MaJiK_420
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Why would they be taped while fruiting? Lol I wrap em in saran wrap and tape the edges while they colonize. Kinda hybrid shotgun/mono.
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MaJiK_420
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19123975 - 11/11/13 11:02 PM (10 years, 2 months ago) |
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I don't use a liner if that's what you mean. or taping the bottom? I thought that was old school and unnecessary lol
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19124002 - 11/11/13 11:09 PM (10 years, 2 months ago) |
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Quote:
MaJiK_420 said: I don't use a liner if that's what you mean. or taping the bottom? I thought that was old school and unnecessary lol
It is old school and since light is not the primary cause of side pins it is unnecessary.
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TeaSippinHippie



Registered: 11/09/13
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Loc: small sticks town
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Re: creeper tub turned PE? [Re: Pastywhyte]
#19124057 - 11/11/13 11:22 PM (10 years, 2 months ago) |
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didn't get the memo of that, all iv ever read was tape and a liner fixes side pinning
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Quote:
TeaSippinHippie said: didn't get the memo of that, all iv ever read was tape and a liner fixes side pinning
Updated
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MaJiK_420
...lost



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Quote:
TeaSippinHippie said: didn't get the memo of that, all iv ever read was tape and a liner fixes side pinning
if you find that it works then by all means go for it. Besides others, I have found tape and liners to be more effort than its worth. Nothing wrong with side pins, sometimes they are ugly, but they are still active lol
in the end do what works for you, methods change and evolve all the time.
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gmc52789


Registered: 09/11/10
Posts: 145
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19124444 - 11/12/13 01:14 AM (10 years, 2 months ago) |
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2 for the price of 1 nice!
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MaJiK_420
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Re: creeper tub turned PE? [Re: gmc52789]
#19124480 - 11/12/13 01:23 AM (10 years, 2 months ago) |
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Yeah I'm just Curious about the next flush now ha.
And then I was thinking, since PE is a mutated strain, does anyone know what the original PE stain is? Or would it vary from vendor to vendor since it's just a mutation? Or am I overthinking that?
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19124697 - 11/12/13 02:47 AM (10 years, 2 months ago) |
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Those are pretty normal side pins for a second flush. Your tub also shows signs of lack of FAE which can lead to presentation of fruits like that, esp on the sides.
While the PE phenotype is possible to find in a multispore grow of any cube, I don't believe that is what you are seeing here.
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MaJiK_420
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Quote:
FrankHorrigan said: Those are pretty normal side pins for a second flush. Your tub also shows signs of lack of FAE which can lead to presentation of fruits like that, esp on the sides.
While the PE phenotype is possible to find in a multispore grow of any cube, I don't believe that is what you are seeing here.
The fruits on the sides don't matter to me, I care less if they come from sides or top at the moment. I admit I only fan maybe twice a day, but I've also experimented with this box leaving the whole thing topless in my gh as well. Always trying something different, my shit aint all dialed in yet lol. And phenotype... I had to look it up, a set of observable characteristics in a individual due do genetic and environmental factors? so that means what? These are just random helmet head, lumpy stemmed mutants rather than"authentic PE"? Lol?
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MaJiK_420
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19126059 - 11/12/13 12:19 PM (10 years, 2 months ago) |
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PussyFart
Retired Cultivation Extrodinaire



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Re: creeper tub turned PE? [Re: MaJiK_420]
#19126062 - 11/12/13 12:20 PM (10 years, 2 months ago) |
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That is PE.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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blojo02184
Big Red



Registered: 05/15/13
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19126070 - 11/12/13 12:22 PM (10 years, 2 months ago) |
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Basically, any p. Cubensis syringe has the genetic material to make a true PE.
the statistical probability is so low that it is very unlikely. The probability that what you see is an environmental condition is alot higher.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: creeper tub turned PE? [Re: blojo02184]
#19126101 - 11/12/13 12:28 PM (10 years, 2 months ago) |
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I almost find it more likely that OP got a mislabeled PE syringe that produced a few normal looking fruits or maybe a mislabled PE6
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MaJiK_420
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Re: creeper tub turned PE? [Re: blojo02184]
#19126115 - 11/12/13 12:31 PM (10 years, 2 months ago) |
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So. I've had one or two pe mutants before, so the significance here to me is that this isn't one or two. This is approximately 30 with a handful of nice full sized pe fruits, from a tub that already flushed creepers, now there are no creeper fruits forming anymore, only pe.
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MaJiK_420
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19126119 - 11/12/13 12:32 PM (10 years, 2 months ago) |
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Creepers or b+ only. I had some actual vendor pe this summer but lost the strain.
This is like 4th or 5th gen expansion g2g from wbs and gs alternating. Really can't remember if it's b+or creepertthough, they looked similar in comparison to the gt I had.
So would this be an environmental factor causing the whole tub to change its genetic expression?
Edited by MaJiK_420 (11/12/13 01:27 PM)
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19126261 - 11/12/13 01:04 PM (10 years, 2 months ago) |
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thats not how generations are defined, thats just expanding the original gen
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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PussyFart
Retired Cultivation Extrodinaire



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Re: creeper tub turned PE? [Re: cronicr]
#19126264 - 11/12/13 01:04 PM (10 years, 2 months ago) |
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--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19126435 - 11/12/13 01:41 PM (10 years, 2 months ago) |
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Quote:
MaJiK_420 said: Pics this morning.



Yeah, that's the PE phenotype alright, or close enough  It actually makes sense that you see those now while the rest of the tub is on its second flush, since PE genetics are very slow fruiters 99% of the time.
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shroomnub4u
college dropout

Registered: 02/27/13
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I think they some say PE originated from the Amazonian. This is not a fact though.
-------------------- “Develop success from failures. Discouragement and failure are two of the surest stepping stones to success.” Dale Carnegie "Whenever you find yourself on the side of the majority, it is time to pause and reflect.” Mark Twain
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MaJiK_420
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So if I take clone sample to agar, would I be likely to get some more penis? Or would I probably need to isolate over time?
Funny that I get a better pe harvest from creepers than I did my first try with actual pe. i just want to keep it going if possible.
I tried looking up PE origins, and couldn't find anything solid. Everyone says mckenna developed it but that's the end. Also that mckenna supposedly worked with amazonian, so it would make sense if he had a batch like I just had and isolated it over time?
Here's another question... If I am taking clone samples, should I sample multiple fruits or only one? I took 8 samples from 1, but there's some that will be ready tomorrow I could whip up another batch of agar
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Gymspawn
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19127916 - 11/12/13 06:20 PM (10 years, 2 months ago) |
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Hey Frank nice Mono is that a test tub or is that how your making your Monos now?
I would give my left dick to have some of the harvests you've had Not the right one though He's my Buddy;)
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MaJiK_420
...lost



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Re: creeper tub turned PE? [Re: Gymspawn]
#19127932 - 11/12/13 06:23 PM (10 years, 2 months ago) |
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lol. ..I think you missed that he had me quoted? Frank hasn't posted any pics here lol
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Gymspawn
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19127969 - 11/12/13 06:30 PM (10 years, 2 months ago) |
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oops well I would give my left dick to have some of your harvests as well you want it??? we'll make a trade He's a bit wore out anyways
Edited by Gymspawn (11/12/13 06:30 PM)
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Gymspawn
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Re: creeper tub turned PE? [Re: Gymspawn]
#19128027 - 11/12/13 06:42 PM (10 years, 2 months ago) |
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Hey and what about the monotub design Majik?
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MaJiK_420
...lost



Registered: 06/30/08
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Re: creeper tub turned PE? [Re: Gymspawn]
#19128066 - 11/12/13 06:50 PM (10 years, 2 months ago) |
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Just an old tub. Was experimenting, just a mono with shotgun holes instead of poly holes. I do my tubs regular mono now, but I found that these still work about as well as my regular monos. they dry out a little faster just cuz they don't have the poly barrier but I open and mist a few times a day and they do fine.
I like to think they get a little increase in fae while the poly tubs get a little increase in humidity retention, they perform equally though it seems, I've gotten wall to wall harvests both versions.
Edited by MaJiK_420 (11/12/13 06:55 PM)
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19128549 - 11/12/13 08:37 PM (10 years, 2 months ago) |
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No one really knows PE's origins. All the stories, from the Vice magazine article to anything else, has just been hearsay.
Penis envy is different in terms of looks but it is a phenotype that is always possible to see in any variety of cubensis. It's just very uncommon so most people choose to start with PE spores.
Take the clone and see what happens. They're very pretty
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Gymspawn
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Great thanks for the phenotype lesson Frank I never knew.
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PussyFart
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Re: creeper tub turned PE? [Re: Gymspawn]
#19128709 - 11/12/13 09:20 PM (10 years, 2 months ago) |
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I wanna know what the spores from those predominantly produce....
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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MaJiK_420
...lost



Registered: 06/30/08
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Re: creeper tub turned PE? [Re: PussyFart]
#19128730 - 11/12/13 09:24 PM (10 years, 2 months ago) |
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Yeah so thats where the confusion comes in, is that pe is a phenotype, not a"strain." Or are stains phenotypes as well? What differentiates a strain and a phenotype? Ha smokin a bowl Damn.
If I were to attempt getting spores... Would I swab? Never done that before but I'd be down to try.
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19128741 - 11/12/13 09:29 PM (10 years, 2 months ago) |
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A phenotype is a broad definition for the general presentation of the fruit/fungus.
A strain in a single set of isolated genetics.
So many strains could present themselves as a single phenotype but only one phenotype will be present in a single strain.
"Variety" is the appropriate term for the name of the cubensis spores, such as Treasure Coast or B+.
Why do spores of certain varities tend towards certain characteristics? I don't really know. Domestication plays a part, that is another area I am not familiar with.
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MaJiK_420
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Can't find a solid spore swab tek, but I keep seeing use a sterile swab... Does it really need to be sterile if it's just going to agar? And I saw one place to soak the swab in water then wipe the gills... is the water necessary?
And thank you Frank, your an encyclopedia ha. good explanation, cleared it up for me
Edited by MaJiK_420 (11/13/13 12:39 AM)
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FrankHorrigan
The Inquisition



Registered: 01/04/11
Posts: 10,573
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Re: creeper tub turned PE? [Re: MaJiK_420]
#19129702 - 11/13/13 01:38 AM (10 years, 2 months ago) |
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Throw some q-tips in a jar, add a lid you would use for grains.
PC it for 30 minutes.
Sterile swabs 
Open the jar in your SAB and take the swabs out using good sterile technique.
Then swab your fruits and move the spores to agar.
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MaJiK_420
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You are The Man Frank. Thanks fir the tips and insights
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