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SamhainJ
I wanna rock out, in my dreams

Registered: 05/16/04
Posts: 1,002
Loc: USA
Last seen: 8 years, 8 months
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
#19123186 - 11/11/13 08:41 PM (10 years, 3 months ago) |
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Post deleted by SamhainJReason for deletion: .
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ImFukNCLUELESS
I SPIT ON PEOPLE


Registered: 11/19/12
Posts: 1,580
Last seen: 9 years, 1 month
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Re: double knockn gran bags [Re: SamhainJ]
#19123194 - 11/11/13 08:43 PM (10 years, 3 months ago) |
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how to pass a drug test FOO MAN'S WBS TEK damions5050's coir tek DONATE TO THE "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf "my girl said it's OK to have a little penis I prefer she didnt have a penis at all"-prisoner#1
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: double knockn gran bags [Re: SamhainJ]
#19123198 - 11/11/13 08:43 PM (10 years, 3 months ago) |
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Quote:
SamhainJ said: Bod, i do t use jars.. bagsonly.. and yes believe it or not my bags have 10% SURFACE area colonized in 24 hours..not 10 % total bag colonization.. and if theres 1000s of diff strains in each syringe why even label em a certain strain for sale? Im sure there could be.tons of diff genetics, but a syringe is made from a specific print amd should be that strain..
You have a lot of reading to do.
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RogerRabbit said: The quote above is horribly inaccurate. It presumes that the name some fool wrote on a print constitutes a strain, which it doesn't. A strain is a pairing of compatible hyphae. One can easily cross spores from prints labeled ecuador and cambodian for example by simply placing a cap to print on top of the print made by the first, and then using agar or a spore syringe. This would not be a hybrid any more than someone named Smith would have a hybrid baby from somebody named Jones. RR
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RogerRabbit said: Isolate more if you want single sectors. You might even start over from scratch, using minimum spores this time. When you have many thousands of strains on the same dish, it can take a lot of transfers.
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Amanita virosa said: The first plate looks like two incompatible strains to me. If they were compatible they would have formed dikaryotic hyphae. But it is really hard to see in that shot. Is there a clear line without Myc where they come together? In other words are they repelling each other? If so they are incompatible strains. You can always just put a small wedge from say five different plates in each jar to increase likelihood of compatibility
Just to clarify something, strains already are dikaryotic. Hyphae are monokaryotic. If compatible mononucleate hyphae(monokaryotic mycelium) meet, they exchange DNA and become polynucleate (dikaryotic). You can't have dikaryotic hyphae as that would be like a two wheeled monocycle. RR
Here's two quotes just so that you can clearly see you have no idea what you're talking about.
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SamhainJ said: Your a fuckin cocksucker and an arrogant prick.. i hope you die.. before i go to bed tonight im gonna pray that something terrible happens to you.. i wish you were local to me so i could shoot the arrogance myself.. im done with thw shroomery bc its all arrogant cocksuckers like you.. wow you are so fuckin co.. i would kill you if given nthe chance..
This is why Obama is our president. God I hope you're to young to vote and drive.
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SamhainJ
I wanna rock out, in my dreams

Registered: 05/16/04
Posts: 1,002
Loc: USA
Last seen: 8 years, 8 months
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
#19123200 - 11/11/13 08:44 PM (10 years, 3 months ago) |
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Post deleted by SamhainJReason for deletion: .
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SamhainJ
I wanna rock out, in my dreams

Registered: 05/16/04
Posts: 1,002
Loc: USA
Last seen: 8 years, 8 months
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
#19123210 - 11/11/13 08:45 PM (10 years, 3 months ago) |
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Post deleted by SamhainJReason for deletion: '
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ImFukNCLUELESS
I SPIT ON PEOPLE


Registered: 11/19/12
Posts: 1,580
Last seen: 9 years, 1 month
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Re: double knockn gran bags [Re: SamhainJ]
#19123217 - 11/11/13 08:46 PM (10 years, 3 months ago) |
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Quote:
SamhainJ said: You do t know shit.. an your whats wrong with this commu ity.. ten years ago we didnt jave a bunch of noob smart asses like you.. just people who wanted to learn there shit.. your a fuckin cunt too..
Dude if you have been here 9 years u should know it take longer than 24hrs for spores to germinate
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how to pass a drug test FOO MAN'S WBS TEK damions5050's coir tek DONATE TO THE "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf "my girl said it's OK to have a little penis I prefer she didnt have a penis at all"-prisoner#1
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Re: double knockn gran bags [Re: SamhainJ]
#19123219 - 11/11/13 08:46 PM (10 years, 3 months ago) |
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Quote:
SamhainJ said: Your a fuckin cocksucker and an arrogant prick.. i hope you die.. before i go to bed tonight im gonna pray that something terrible happens to you.. i wish you were local to me so i could shoot the arrogance myself.. im done with thw shroomery bc its all arrogant cocksuckers like you.. wow you are so fuckin co.. i would kill you if given nthe chance..
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SamhainJ said: 1slw wtf do you know, your asking if innoculating twoce will hurt your bags bit then tirn around and act like a pro mycoligist . Eat my ass
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SamhainJ said: You do t know shit.. an your whats wrong with this commu ity.. ten years ago we didnt jave a bunch of noob smart asses like you.. just people who wanted to learn there shit.. your a fuckin cunt too..
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SamhainJ said: I was trying to help bit your just a stupid.littl cunt.. yes inocing your bags twice will cause an explosion you fucking fag
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SamhainJ
I wanna rock out, in my dreams

Registered: 05/16/04
Posts: 1,002
Loc: USA
Last seen: 8 years, 8 months
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
#19123222 - 11/11/13 08:47 PM (10 years, 3 months ago) |
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Post deleted by SamhainJReason for deletion: Bods butt is far too hurt
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ImFukNCLUELESS
I SPIT ON PEOPLE


Registered: 11/19/12
Posts: 1,580
Last seen: 9 years, 1 month
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Re: double knockn gran bags [Re: bodhisatta]
#19123226 - 11/11/13 08:48 PM (10 years, 3 months ago) |
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I like u samhainj u made me lol literally
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how to pass a drug test FOO MAN'S WBS TEK damions5050's coir tek DONATE TO THE "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf "my girl said it's OK to have a little penis I prefer she didnt have a penis at all"-prisoner#1
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SamhainJ
I wanna rock out, in my dreams

Registered: 05/16/04
Posts: 1,002
Loc: USA
Last seen: 8 years, 8 months
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
#19123227 - 11/11/13 08:48 PM (10 years, 3 months ago) |
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Post deleted by SamhainJReason for deletion: Drama
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ImFukNCLUELESS
I SPIT ON PEOPLE


Registered: 11/19/12
Posts: 1,580
Last seen: 9 years, 1 month
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Re: double knockn gran bags [Re: SamhainJ]
#19123238 - 11/11/13 08:50 PM (10 years, 3 months ago) |
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Man u just need to calm down there is no need to get so easliy butthurt buddy it will be ok smoke some weed chill out
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how to pass a drug test FOO MAN'S WBS TEK damions5050's coir tek DONATE TO THE "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf "my girl said it's OK to have a little penis I prefer she didnt have a penis at all"-prisoner#1
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Citric


Registered: 03/19/04
Posts: 4,490
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Clean this up guys. One ban has been handed out. Thanks to those who kept a cool head.
-------------------- Self Healing lid tek ** Update 10.17.17 ** Mini casing pictures: Pins to harvest Cup O' Shrooms Magash: I noticed my contams were in the shape of fingers Hyphae: Yes "Loss of moisture from the substrate" is not a casing trigger. My final Grow!
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Gymspawn
Stranger
Registered: 11/10/13
Posts: 100
Last seen: 8 years, 1 month
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Re: double knockn gran bags [Re: Citric]
#19123347 - 11/11/13 09:05 PM (10 years, 3 months ago) |
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It's ok don't be Butthurt.
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TeaSippinHippie



Registered: 11/09/13
Posts: 621
Loc: small sticks town
Last seen: 3 years, 3 months
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Re: double knockn gran bags [Re: Gymspawn]
#19123943 - 11/11/13 10:54 PM (10 years, 3 months ago) |
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aye man, it shouldn't hurt it if the bags not already really wet, well at least I don't think from what I've read. im just trying to help another shroomer out, as it is hard to get help recently on here.
-------------------- I LOVE THE SHROOMERY, GOODVIBES TO EVERYONE. MudaFuka said: There's nothing wrong with making mistakes as long as you don't make the same ones twice.
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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any luck 1slow2v ?
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ImFukNCLUELESS
I SPIT ON PEOPLE


Registered: 11/19/12
Posts: 1,580
Last seen: 9 years, 1 month
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Re: double knockn gran bags [Re: bodhisatta]
#19141972 - 11/15/13 06:07 PM (10 years, 3 months ago) |
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Dont gonna check when i get home if not guess im gonna go back to doing it myseld
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how to pass a drug test FOO MAN'S WBS TEK damions5050's coir tek DONATE TO THE "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf "my girl said it's OK to have a little penis I prefer she didnt have a penis at all"-prisoner#1
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Wow some drama up in this beatch, good on bodhisatta keepin shit real 
OP when it comes to inoculating grain with spores, shit can take time. I have had jars that I gave up on, but was too lazy to pitch end up germinating after a month and produce fruits. I personally think people pitch shit too fast if it does not germinate. I also find that spores to grains are a crapshoot anyway and its much easier to achieve germination on agar 
Even on agar stuff sometimes takes a while. Once I got a print in a trade and it was the worst, faintest print ever. I decided to give it a try and after 3 weeks and nothing, I figured it was no good. But I never pitched the plate, and forgot about it. 6 months later and I'm cleaning my lab and I come across the plate that I forgot. Look inside and lo and behold I see some germination, couldn't have been more than a few days old. Figured it had to be a contam. But a few days later and then a transfer and I had some nice rhizomorphic mycelium. Couldn't believe the plate still had enough moisture to facilitate germination.
I'm not saying that grains could ever do this, chances are some endospores that might have made it through the PC cycle will have recovered by then and fuck the jars. But IME don't start to worry because its been 2 weeks with no growth, it still might come. I would never reinoculate as that becomes a second vector for contams.
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Moorning Due
been know to derp


Registered: 10/08/13
Posts: 8,061
Loc: ether jet of existence
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Re: double knockn gran bags [Re: Pastywhyte]
#19178561 - 11/23/13 11:29 AM (10 years, 3 months ago) |
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I just committed suicide because of this thread.
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