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OfflineImFukNCLUELESS
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double knockn gran bags
    #19122818 - 11/11/13 07:52 PM (10 years, 2 months ago)

I kncked some grain bags about 1 1/2 weeks ago have seen nothing thinking about trying to inoculate them again could this cause a problem:shrug:


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Offlinefrenchfries
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Re: double knockn gran bags [Re: ImFukNCLUELESS]
    #19122834 - 11/11/13 07:53 PM (10 years, 2 months ago)

I would stop wasting spores on the bags and trust yourself to use the pc.  You can do it!!!:smirk:


--------------------
"Oh Krishna I have no desire for victory or for a kingdom or pleasures.' "Even if they were to kill me I would not want to kill them not even to become the ruler of the world."


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OfflineSamhainJ
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Re: double knockn gran bags [Re: frenchfries]
    #19122895 - 11/11/13 08:01 PM (10 years, 2 months ago)

I knocked a grain spawn bag up a week ago with 1 cc pf red spore strain.. and only like 2 grains are colinzing every other day.. its a one pound bag.. think i need to hit it with more or innoc it with a faster colonizing strain..


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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: frenchfries]
    #19122947 - 11/11/13 08:07 PM (10 years, 2 months ago)

Quote:

frenchfries said:
I would stop wasting spores on the bags and trust yourself to use the pc.  You can do it!!!:smirk:




I typically do guess cuttng corners trying to save time  using pre sterilized bags is gonna bite me on the ass


--------------------
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I prefer she didnt have a penis at all"-prisoner#1


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InvisiblebodhisattaMDiscordReddit
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Re: double knockn gran bags [Re: SamhainJ]
    #19122992 - 11/11/13 08:13 PM (10 years, 2 months ago)

Quote:

1slow2v said:
I kncked some grain bags about 1 1/2 weeks ago have seen nothing thinking about trying to inoculate them again could this cause a problem:shrug:




how much spore solution did you use, how big is the bag, and did you shake/mush it after you inoculated it?

Quote:

frenchfries said:
I would stop wasting spores on the bags and trust yourself to use the pc.  You can do it!!!:smirk:



maybe he made his own grain bags with a PC :shrug:
-Edit- nvm just saw the reply.
Premade bags do suck but it's hard to say that's your problem.

Quote:

SamhainJ said:
I knocked a grain spawn bag up a week ago with 1 cc pf red spore strain.. and only like 2 grains are colinzing every other day.. its a one pound bag.. think i need to hit it with more or innoc it with a faster colonizing strain..




It just started why are you worrying? You need to sit and be patient. 1 week is appropriate and of course it starts with one grain. Shake that bag up once it's 20-40% colonized.

More inoculant isn't going to speed anything up and it might slow it down. Less spores is better so you have less strains. A strain is two spores mating. The only way you'll find a faster colonizing strain is to isolate mycellium on agar and select an isolate(single strain) that is a strong performer.


Edited by Trusted cuItivator (11/11/13 08:14 PM)


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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: bodhisatta]
    #19123035 - 11/11/13 08:18 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: Burth-hurtedness from someone who thinks there a pro but is just arrogant..



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InvisiblebodhisattaMDiscordReddit
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Re: double knockn gran bags [Re: SamhainJ]
    #19123051 - 11/11/13 08:21 PM (10 years, 2 months ago)

Could just be that that one bag has been prepared too wet or too dry. A picture might help. Could also have been too little spores but idk how much you used.
I would think since all the others did well that the 1 pound bag has a fault.


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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
    #19123055 - 11/11/13 08:22 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: Ass hurt dbags



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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: SamhainJ]
    #19123061 - 11/11/13 08:24 PM (10 years, 2 months ago)

I used abour 1.5ml per 2lb bag 3 bags total no signs in any of them and did not shake the bag


--------------------
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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: ImFukNCLUELESS]
    #19123067 - 11/11/13 08:25 PM (10 years, 2 months ago)

My spore syringe was made in july could that be the probkem


--------------------
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Re: double knockn gran bags [Re: SamhainJ]
    #19123076 - 11/11/13 08:25 PM (10 years, 2 months ago)

Quote:

SamhainJ said:
And appearance of mycelium cant possibly be just from two spores germinating.. bc on average i have about 10 percent colinization of the surface substrate in 24 hours.. and its not contams.. my frst grow was in 2002.. i know the diff between myc and comtams.. i jist have great colinzation temps etc




who said that? You have 1000's of strains growing on your grains if you use spore syringes.

10% of the jar is colonized in 24h after you shoot spores in?
BS
10% of the jar is colonized in 24h after you see the first visible sign of myc
BS


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InvisiblebodhisattaMDiscordReddit
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Re: double knockn gran bags [Re: ImFukNCLUELESS]
    #19123089 - 11/11/13 08:26 PM (10 years, 2 months ago)

Quote:

1slow2v said:
I used abour 1.5ml per 2lb bag 3 bags total no signs in any of them and did not shake the bag




Weird. I mean spores can take up to 2 weeks. I would have to say if those same spores worked in other bags it's the bags being prepared incorrectly.


Quote:

1slow2v said:
My spore syringe was made in july could that be the probkem




nope. Even at room temperature it would still be just fine.


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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
    #19123094 - 11/11/13 08:27 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: A pussy got butthurt



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InvisiblebodhisattaMDiscordReddit
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Re: double knockn gran bags [Re: SamhainJ]
    #19123105 - 11/11/13 08:29 PM (10 years, 2 months ago)

Quote:

SamhainJ said:
I do t believe it too be too wet or dry, it seems normal.. ive never worked with this strain excwpt this one bag, i know a cube is a cube.. but sometimes it isnt.. due to people i share a home with my house isnt as clean as it would be so im pretty experienced with bags and had great success besidea thos pf redspore bag.. i dont grow dry pounds at a time but i def get enough to keep myself and close friends tripping all year..bags are good for some.. but i believe its cooler if you make your own..



Understandable English is cool too. are tripping and texting to the shroomery on a phone?


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OfflineSamhainJ
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Re: double knockn gran bags [Re: SamhainJ]
    #19123121 - 11/11/13 08:32 PM (10 years, 2 months ago)

Bod, i do t use jars.. bagsonly.. and yes believe it or not my bags have 10% SURFACE area colonized in 24 hours..not 10 % total bag colonization.. and if theres 1000s of diff strains in each syringe why even label em a certain strain for sale? Im sure there could be.tons of diff genetics, but a syringe is made from a specific print amd should be that strain..


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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: SamhainJ]
    #19123134 - 11/11/13 08:33 PM (10 years, 2 months ago)

Its not indiviual gentics like it would be from an isolate

And i agree yhere is no way u have 10% surface area colonized in 24hrs


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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
    #19123146 - 11/11/13 08:35 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: Drama for yo mama



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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: SamhainJ]
    #19123173 - 11/11/13 08:39 PM (10 years, 2 months ago)

Ooooo is your pussy hurting


--------------------
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FOO MAN'S WBS TEK
damions5050's coir tek
DONATE TO THE  "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf

"my girl said it's OK to have a little penis
I prefer she didnt have a penis at all"-prisoner#1


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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
    #19123180 - 11/11/13 08:40 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: Post made someone butthurt



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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: ImFukNCLUELESS]
    #19123182 - 11/11/13 08:41 PM (10 years, 2 months ago)

Did your daddy touch u when u were younger

It will be ok


--------------------
how to pass a drug test
FOO MAN'S WBS TEK
damions5050's coir tek
DONATE TO THE  "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf

"my girl said it's OK to have a little penis
I prefer she didnt have a penis at all"-prisoner#1


Edited by ImFukNCLUELESS (11/11/13 08:41 PM)


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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
    #19123186 - 11/11/13 08:41 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: .



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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: SamhainJ]
    #19123194 - 11/11/13 08:43 PM (10 years, 2 months ago)

:canthelpbutlaugh:


--------------------
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damions5050's coir tek
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"my girl said it's OK to have a little penis
I prefer she didnt have a penis at all"-prisoner#1


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Re: double knockn gran bags [Re: SamhainJ]
    #19123198 - 11/11/13 08:43 PM (10 years, 2 months ago)

Quote:

SamhainJ said:
Bod, i do t use jars.. bagsonly.. and yes believe it or not my bags have 10% SURFACE area colonized in 24 hours..not 10 % total bag colonization.. and if theres 1000s of diff strains in each syringe why even label em a certain strain for sale? Im sure there could be.tons of diff genetics, but a syringe is made from a specific print amd should be that strain..




You have a lot of reading to do.
Quote:

RogerRabbit said:
The quote above is horribly inaccurate.  It presumes that the name some fool wrote on a print constitutes a strain, which it doesn't.  A strain is a pairing of compatible hyphae.  One can easily cross spores from prints labeled ecuador and cambodian for example by simply placing a cap to print on top of the print made by the first, and then using agar or a spore syringe.  This would not be a hybrid any more than someone named Smith would have a hybrid baby from somebody named Jones.
RR



Quote:

RogerRabbit said:
Isolate more if you want single sectors.  You might even start over from scratch, using minimum spores this time.  When you have many thousands of strains on the same dish, it can take a lot of transfers.

Quote:

Amanita virosa said:
The first plate looks like two incompatible strains to me. If they were compatible they would have formed dikaryotic hyphae. But it is really hard to see in that shot. Is there a clear line without Myc where they come together?  In other words are they repelling each other?  If so they are incompatible strains. You can always just put a small wedge from say five different plates in each jar to increase likelihood of compatibility




Just to clarify something, strains already are dikaryotic.  Hyphae are monokaryotic.  If compatible mononucleate hyphae(monokaryotic mycelium) meet, they exchange DNA and become polynucleate (dikaryotic).  You can't have dikaryotic hyphae as that would be like a two wheeled monocycle.
RR




Here's two quotes just so that you can clearly see you have no idea what you're talking about.

Quote:

SamhainJ said:
Your a fuckin cocksucker and an arrogant prick.. i hope you die.. before i go to bed tonight im gonna pray that something terrible happens to you.. i wish you were local to me so i could shoot the arrogance myself.. im done with thw shroomery bc its all arrogant cocksuckers like you.. wow you are so fuckin co.. i would kill you if given nthe chance..




This is why Obama is our president. God I hope you're to young to vote and drive.
:bye:


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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
    #19123200 - 11/11/13 08:44 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: .



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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
    #19123210 - 11/11/13 08:45 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: '



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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: SamhainJ]
    #19123217 - 11/11/13 08:46 PM (10 years, 2 months ago)

Quote:

SamhainJ said:
You do t know shit.. an  your whats wrong with this commu ity.. ten years ago we didnt jave a bunch of noob smart asses like you.. just people who wanted to learn there shit.. your a fuckin cunt too..



Dude if you have been here 9 years u should know it take longer than 24hrs for spores to germinate


--------------------
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I prefer she didnt have a penis at all"-prisoner#1


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Re: double knockn gran bags [Re: SamhainJ]
    #19123219 - 11/11/13 08:46 PM (10 years, 2 months ago)

Quote:

SamhainJ said:
Your a fuckin cocksucker and an arrogant prick.. i hope you die.. before i go to bed tonight im gonna pray that something terrible happens to you.. i wish you were local to me so i could shoot the arrogance myself.. im done with thw shroomery bc its all arrogant cocksuckers like you.. wow you are so fuckin co.. i would kill you if given nthe chance..



Quote:

SamhainJ said:
1slw wtf do you know, your asking if innoculating twoce will hurt your bags bit then tirn around and act like a pro mycoligist
. Eat my ass



Quote:

SamhainJ said:
You do t know shit.. an  your whats wrong with this commu ity.. ten years ago we didnt jave a bunch of noob smart asses like you.. just people who wanted to learn there shit.. your a fuckin cunt too..



Quote:

SamhainJ said:
I was trying to help bit your just a stupid.littl cunt.. yes inocing your bags twice will cause an explosion you fucking fag





:smbfacepalm:


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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
    #19123222 - 11/11/13 08:47 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: Bods butt is far too hurt



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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: bodhisatta]
    #19123226 - 11/11/13 08:48 PM (10 years, 2 months ago)

I like u samhainj u made me lol literally


--------------------
how to pass a drug test
FOO MAN'S WBS TEK
damions5050's coir tek
DONATE TO THE  "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf

"my girl said it's OK to have a little penis
I prefer she didnt have a penis at all"-prisoner#1


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OfflineSamhainJ
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Re: double knockn gran bags *DELETED* [Re: SamhainJ]
    #19123227 - 11/11/13 08:48 PM (10 years, 2 months ago)

Post deleted by SamhainJ

Reason for deletion: Drama



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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: SamhainJ]
    #19123238 - 11/11/13 08:50 PM (10 years, 2 months ago)

Man u just need to calm down there is no need to get so easliy butthurt buddy it will be ok smoke some weed chill out


--------------------
how to pass a drug test
FOO MAN'S WBS TEK
damions5050's coir tek
DONATE TO THE  "IM FUCKING BROKE FUND" 1PtqhURaxtCpGpeUBNqeZi7XnmKwWe8WWf

"my girl said it's OK to have a little penis
I prefer she didnt have a penis at all"-prisoner#1


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InvisibleCitric
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Re: double knockn gran bags [Re: ImFukNCLUELESS]
    #19123260 - 11/11/13 08:53 PM (10 years, 2 months ago)

Clean this up guys.  One ban has been handed out.  Thanks to those who kept a cool head.


--------------------
Self Healing lid tek ** Update 10.17.17 **
Mini casing pictures: Pins to harvest
Cup O' Shrooms
Magash:  I noticed my contams were in the shape of fingers :whack:
Hyphae:  Yes  "Loss of moisture from the substrate"  is not a casing trigger.  :cuckoo:

My final Grow!


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OfflineGymspawn
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Re: double knockn gran bags [Re: Citric]
    #19123347 - 11/11/13 09:05 PM (10 years, 2 months ago)

It's ok don't be Butthurt.


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Re: double knockn gran bags [Re: Gymspawn]
    #19123943 - 11/11/13 10:54 PM (10 years, 2 months ago)

aye man, it shouldn't hurt it if the bags not already really wet, well at least I don't think from what I've read. im just trying to help another shroomer out, as it is hard to get help recently on here.


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Re: double knockn gran bags [Re: TeaSippinHippie]
    #19140533 - 11/15/13 11:10 AM (10 years, 2 months ago)

any luck 1slow2v ?


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OfflineImFukNCLUELESS
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Re: double knockn gran bags [Re: bodhisatta]
    #19141972 - 11/15/13 06:07 PM (10 years, 2 months ago)

Dont gonna check when i get home if not guess im gonna go back to doing it myseld


--------------------
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Re: double knockn gran bags [Re: ImFukNCLUELESS]
    #19142066 - 11/15/13 06:28 PM (10 years, 2 months ago)

Wow some drama up in this beatch, good on bodhisatta keepin shit real :super:

OP when it comes to inoculating grain with spores, shit can take time. I have had jars that I gave up on, but was too lazy to pitch end up germinating after a month and produce fruits. I personally think people pitch shit too fast if it does not germinate. I also find that spores to grains are a crapshoot anyway and its much easier to achieve germination on agar :awesomenod:

Even on agar stuff sometimes takes a while. Once I got a print in a trade and it was the worst, faintest print ever. I decided to give it a try and after 3 weeks and nothing, I figured it was no good. But I never pitched the plate, and forgot about it. 6 months later and I'm cleaning my lab and I come across the plate that I forgot. Look inside and lo and behold I see some germination, couldn't have been more than a few days old. Figured it had to be a contam. But a few days later and then a transfer and I had some nice rhizomorphic mycelium. Couldn't believe the plate still had enough moisture to facilitate germination.

I'm not saying that grains could ever do this, chances are some endospores that might have made it through the PC cycle will have recovered by then and fuck the jars. But IME don't start to worry because its been 2 weeks with no growth, it still might come. I would never reinoculate as that becomes a second vector for contams.


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InvisibleMoorning Due
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Registered: 10/08/13
Posts: 8,061
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Re: double knockn gran bags [Re: Pastywhyte]
    #19178561 - 11/23/13 11:29 AM (10 years, 2 months ago)

I just committed suicide because of this thread.


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