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dh0372
Stranger

Registered: 11/01/13
Posts: 8
Last seen: 10 years, 2 months
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Smoke on the water
#19119508 - 11/11/13 09:10 AM (10 years, 2 months ago) |
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Smoke on the water.
This is my third try in getting liquid culture. The first two where with poor man mycelium. It didn't work!!!!!
This time I tried two different teks. One from spors,and the other form spore syringe. The spores where made in the 26th now it is the 11th of the Next mount 14-15 days. The second one,was made in the 3rd now is the 11th 9 days ,and what I see is a white cloud swimming in the Jar like---- smoke on the water%%%% maybe this is what deep purple were talking about. Fellow shroomers,yes. I think this time I got it right What do you think?
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Kiya_Star427
Live FREE or be a SLAVE!


Registered: 10/14/13
Posts: 355
Loc: New York
Last seen: 5 years, 1 month
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Re: Smoke on the water [Re: dh0372]
#19119554 - 11/11/13 09:24 AM (10 years, 2 months ago) |
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and fire in the sky!
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 18 hours, 4 minutes
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I think you should look into agar and forget about lc for now. When you become more experienced and more confident in your sterile technique you could try lc again.
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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LC is a hard way to learn.
more and more pros, are recommending agar.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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LCs from spores are sketchy as hell....and are known for their failure rate.
If you really want to learn how to expand your mycellium, look into agar and G2G transfers.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: Smoke on the water [Re: PussyFart]
#19120747 - 11/11/13 02:00 PM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: LCs known for their failure rate.
that one educational sentence, says it all.
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dutchfunkle
subowski


Registered: 02/10/11
Posts: 132
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Re: Smoke on the water [Re: PussyFart]
#19121301 - 11/11/13 03:41 PM (10 years, 2 months ago) |
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O RLY? Ive had 100% success anytime Ive done LC from spore syringe. The rhetoric around here is SO annoying when you take one mans word and put it on a pedestal as if there is no other alternative. Keep it up OP and dont let your failures keep you down, when the time comes youre ready for agar, then go for it. You should look into grain LC if contamination keeps getn the best of ya.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Quote:
dutchfunkle said: O RLY? Ive had 100% success anytime Ive done LC from spore syringe. The rhetoric around here is SO annoying when you take one mans word and put it on a pedestal as if there is no other alternative.
Use the search function bro.
It has nothing to do with one man's word....
It does however have to do with a terrible success rate from litterally thousands of noobs.
The cap from which the spores came was most likely fruited in open air.
Which means it is more than likely not 100% clean.
Knowing this, we then inoculate a mixture of sugar and water, which is the perfect breeding ground for everything nasty.
Quote:
dutchfunkle said: Keep it up OP and dont let your failures keep you down, when the time comes youre ready for agar, then go for it. You should look into grain LC if contamination keeps getn the best of ya.
GLC is no less risky.
You cannot inspect every grain in the jar.
There could be one contaminated grain in the middle that you cannot see, and you just made a contaminated LC.
The best way to start an LC is from a clean agar wedge.
But going from agar to LC is just wasting time IMHO.
You could just use the wedge to inoculate a master jar, then G2G it out, never having touched a syringe, and not having to wait for an LC to colonize, only to inoculate grain jars anyways.
Agar straight to grains saves so much time when compared to making a LC....and is far less risky, because there is less involved.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Gymspawn
Stranger
Registered: 11/10/13
Posts: 100
Last seen: 8 years, 8 days
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Re: Smoke on the water [Re: PussyFart]
#19121426 - 11/11/13 03:59 PM (10 years, 2 months ago) |
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I've only had success with GLC LC fucked me all the way to sunday everytime
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silverstem
Caps & Stems



Registered: 10/12/13
Posts: 900
Loc: jordan
Last seen: 6 years, 5 months
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Re: Smoke on the water [Re: Gymspawn]
#19121958 - 11/11/13 05:34 PM (10 years, 2 months ago) |
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i just went from ager wedge to lc... only reason im doing that is because im using brf cakes as spawn... if i was doing grains i would do agar to grain.. then g2g. ive tried ms to lc and failed...
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Shroomery needs a gun forum!!!!!!!!! CAN WE HAVE ONE?????
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dh0372
Stranger

Registered: 11/01/13
Posts: 8
Last seen: 10 years, 2 months
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first of all i dont have a p-corker so stop all this talk about g2g and stuff not relevant.
second i did try agar the same day that i did the lc. guess what it has been two weeks their is nothing on the agar plates
6 of them not even Bactria absolutely nothing.
at the same time something is happening in the lc.
The cap from which the spores came was most likely fruited in open air. the cap was fruited in a terrarium thanks very much.
i didnt even get one relevant answer did you look at the pictures,how does it looks to you,good or not? it is a simple question inst it.
it seems to me that people around here are more interested in barking at somebody then answering questions.
thanks dutchfunkle for the only descent answer.
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 18 hours, 4 minutes
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Re: Smoke on the water [Re: dh0372]
#19124105 - 11/11/13 11:37 PM (10 years, 2 months ago) |
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Rubestoad
Stranger


Registered: 09/13/13
Posts: 231
Loc: your mamas porch. sunrise
Last seen: 8 years, 3 months
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Re: Smoke on the water [Re: dh0372]
#19124240 - 11/12/13 12:23 AM (10 years, 2 months ago) |
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Quote:
dh0372 said: first of all i dont have a p-corker so stop all this talk about g2g and stuff not relevant.
second i did try agar the same day that i did the lc. guess what it has been two weeks their is nothing on the agar plates
6 of them not even Bactria absolutely nothing.
at the same time something is happening in the lc.
The cap from which the spores came was most likely fruited in open air the cap was fruited in a terrarium thanks very much.
i didnt even get one relevant answer did you look at the pictures,how does it looks to you,good or not? it is a simple question inst it.
it seems to me that people around here are more interested in barking at somebody then answering
thanks dutchfunkle for the only descent answer.
Tried agar without a pressure cooker?
The answer to your simple question.... Looking at a LC will not tell you if it's contamed.
These peeps are trying to help you but if you don't like the answers prove them wrong.
Or after it doesn't work make a new account and try to be more humble
-------------------- Go placidly amid the noise and haste, and remember what peace there may be in silence.
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Smoke on the water [Re: dh0372]
#19124290 - 11/12/13 12:37 AM (10 years, 2 months ago) |
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Quote:
dh0372 said: first of all i dont have a p-corker so stop all this talk about g2g and stuff not relevant.
second i did try agar the same day that i did the lc. guess what it has been two weeks their is nothing on the agar plates
6 of them not even Bactria absolutely nothing.
at the same time something is happening in the lc.
The cap from which the spores came was most likely fruited in open air. the cap was fruited in a terrarium thanks very much.
i didnt even get one relevant answer did you look at the pictures,how does it looks to you,good or not? it is a simple question inst it.
it seems to me that people around here are more interested in barking at somebody then answering questions.
thanks dutchfunkle for the only descent answer.
Your 7th post.... I think at my 7th post I felt the same... Was always being corrected, referred back to rules, or asked to use the search engine, or asked to do more reading. Thanks guys! I did me well I think...
I'm glad I stuck around
And btw, the problem with LC is forget the picture, the only way to know if your LC is good is to knock-up agar plates and see what will happens... I think it is standard practice for anyone trying an LC to knockup a plate with it to see if it is good, then show a picture of the plate, and that should say very mucho about the LC you have.
Good luck
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Re: Smoke on the water [Re: dh0372]
#19124302 - 11/12/13 12:40 AM (10 years, 2 months ago) |
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Quote:
dh0372 said: The cap from which the spores came was most likely fruited in open air. the cap was fruited in a terrarium thanks very much.
And the terrarium was in a SAB or in front of a flow hood?
No, it was in open air....and whatever air was inside the terrarium was filled with contams.
You are very welcome.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Smoke on the water [Re: jpack666]
#19124313 - 11/12/13 12:41 AM (10 years, 2 months ago) |
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Quote:
Or after it doesn't work make a new account and try to be more humble
Lol... Stick around, it's true everyone here is helpful... If they seem harsh, it's probable for a good reason. Maybe they beleive this is what can help you the most.
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Smoke on the water [Re: PussyFart]
#19124326 - 11/12/13 12:43 AM (10 years, 2 months ago) |
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Quote:
Notahacker420 said:
Quote:
dh0372 said: The cap from which the spores came was most likely fruited in open air. the cap was fruited in a terrarium thanks very much.
And the terrarium was in a SAB or in front of a flow hood?
No, it was in open air....and whatever air was inside the terrarium was filled with contams.
You are very welcome.
Very true... Read http://www.shroomery.org/forums/showflat.php/Number/19035259 That's what made me understand... Can't beleive the generosity of people here!
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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dutchfunkle
subowski


Registered: 02/10/11
Posts: 132
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Re: Smoke on the water [Re: jpack666]
#19125305 - 11/12/13 07:55 AM (10 years, 2 months ago) |
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Anytime OP! No pressure cooker often ends in frustration. Go down to your local thrift store or goodwill and look for Pressure Cookers, they dont have to be the biggest or baddest, opt for a small 7 quart mirro thats half a century old. These are a must for sterile conditions for cultures to thrive. After you get your PC,order your favorite edible spore syringe from a reputable sponser here and keep reading posts on here. To avoid all of this headache on the cultivation forum, make the search function your best friend;Any questions you have,type it in the search box and you can even narrow your searches by checking a box for Trusted Cultivators to weed out all the B.S. Hope this helps ya bud...
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dh0372
Stranger

Registered: 11/01/13
Posts: 8
Last seen: 10 years, 2 months
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you new the Sentence you dont need a weather man to know where the wind blow.
well you dont need to be an expert shromer to know that you have been answered by bad people. how just want to lash at you.
if you dont like the question dont answer it.
if you find it annoying move to another post.
and above all try to be more of a human been and less off an ass.
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anne halonium
jaguarette


Registered: 05/07/13
Posts: 1,908
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Re: Smoke on the water [Re: dh0372]
#19130328 - 11/13/13 08:33 AM (10 years, 2 months ago) |
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i like these threads, where if ya dont agree with a half baked grow op approach, then somehow your from outer space.
all serious growers, should just cease to entertain peeps without PC's it wastes everyones time, and encourages the lowest form of grow.
LC's are a known to be risky. anyone claiming total 100% success always with them, is essentially saying they had 1 lucky grow.
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