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WindSearcher
Stranger

Registered: 07/15/13
Posts: 33
Last seen: 8 years, 2 months
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Part of jar not colonizing
#19109059 - 11/09/13 05:03 AM (10 years, 3 months ago) |
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So I've got some pf jars that are all fully colonized and have been consolidating for a few days now. Only this jar has a part on the bottom that will not colonize, the myc near the spot is really dense and there is some yellowish metabolites around it I think. Should I just give it some more time? (pic is a bit blurry)
-------------------- -Space does not offer truth and neither does it lie-
Edited by WindSearcher (11/09/13 05:05 AM)
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JMcDoogle
A Serious Scholar


Registered: 07/07/09
Posts: 1,495
Loc: Nunavut
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I am going to take a stab at saying its contamed.
If you have others, toss it.
If you live in a region where it still warm out,
throw it in the garden.
Something will come of it.
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The ego is nothing other than the focus of conscious attention.
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Skinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
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Re: Part of jar not colonizing [Re: JMcDoogle]
#19109194 - 11/09/13 06:23 AM (10 years, 3 months ago) |
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Probably wet spot
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WindSearcher
Stranger

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Re: Part of jar not colonizing [Re: Skinty]
#19109588 - 11/09/13 09:00 AM (10 years, 3 months ago) |
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Will myc colonize a wet spot over time? And is it a contamination risk?
-------------------- -Space does not offer truth and neither does it lie-
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Skinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
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Quote:
WindSearcher said: Will myc colonize a wet spot over time? And is it a contamination risk?
NO I don't think so. You could cut it out if you wanted and still fruit. It has already contam'd you cake so yeh it's a risk
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spacechildo
proletarians rise up



Registered: 01/24/13
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Re: Part of jar not colonizing [Re: Skinty]
#19109697 - 11/09/13 09:42 AM (10 years, 3 months ago) |
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doesn't look like cube mycelium to me. bad pic but still.. what are you growing?
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WindSearcher
Stranger

Registered: 07/15/13
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Re: Part of jar not colonizing [Re: spacechildo]
#19109798 - 11/09/13 10:15 AM (10 years, 3 months ago) |
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should be p. cubensis (thai strain), the mycelium around the spot is a bit weird, but the rest and the other jars look like they should imo
-------------------- -Space does not offer truth and neither does it lie-
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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your subs compact so your myc is working harder,loosen your lid a bit and if that doesnt help flip it
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Skinty
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Registered: 07/04/13
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Re: Part of jar not colonizing [Re: cronicr]
#19109850 - 11/09/13 10:33 AM (10 years, 3 months ago) |
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Quote:
cronicr said: your subs compact so your myc is working harder,loosen your lid a bit and if that doesnt help flip it
You reckon? That thick "solid white" looking myc that does not move is a pretty good indicator of wet spot / bac of some sort. Don't think sub is likely to be significantly more packed in one place than another - especially at the bottom of the jar
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
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Re: Part of jar not colonizing [Re: Skinty]
#19109879 - 11/09/13 10:47 AM (10 years, 3 months ago) |
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on my commp now and it does look off, but if a jar is gonna compact it would naturally happen at the bottom where gravity draws moisture/compacts the bottom making the myc work that much harder to penetrate the uncolonized sub so you often see an excess of metabolites and ti9ck mycelium around the uncolonized portion of the jar, were these widee muth 1/2 pints?
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Skinty
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Re: Part of jar not colonizing [Re: cronicr]
#19109894 - 11/09/13 10:53 AM (10 years, 3 months ago) |
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Quote:
cronicr said: on my commp now and it does look off, but if a jar is gonna compact it would naturally happen at the bottom where gravity draws moisture/compacts the bottom making the myc work that much harder to penetrate the uncolonized sub so you often see an excess of metabolites and ti9ck mycelium around the uncolonized portion of the jar, were these widee muth 1/2 pints?
Ahh I see your point. I was thinking more that people wrongly sometimes pack their sub in the jar which would mean more compact at the top
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cronicr



Registered: 08/07/11
Posts: 61,436
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Re: Part of jar not colonizing [Re: Skinty]
#19109918 - 11/09/13 11:00 AM (10 years, 3 months ago) |
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  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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anon788
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Registered: 11/08/13
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Re: Part of jar not colonizing [Re: cronicr]
#19110044 - 11/09/13 11:38 AM (10 years, 3 months ago) |
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Could possibly be related to your GE.
When I use a LC/syringe filter for a cake, this always happens. (Yes I know syringe filter is for LC and not cakes; and what I'm explaining is exactly why you don't use these for cakes).
But it always presents like this. Regardless of # of inoculation points. The area close to the GE port always grows good. The farthest distance from the GE always ends up stalling out.
Though OP probably not using a LC filter, and the 'hard edges' I can see from the bad pic really shout contam or water collecting.
Edited by anon788 (11/09/13 11:40 AM)
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WindSearcher
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Re: Part of jar not colonizing [Re: anon788]
#19119022 - 11/11/13 04:36 AM (10 years, 3 months ago) |
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just checked on the jar and it is almost 100% colonized now. The myc near the spot still looks a bit weird, but no signs of other contams. Will it be safe to fruit it with the other jars in the SGFC after it's 100% colonized?
-------------------- -Space does not offer truth and neither does it lie-
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sytar
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Registered: 09/01/13
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Looked fine to me, just monokaryotic mycelial growth stalling near the bottom. Smell it when you birth but it should be fine IMO (though I am a newb).
-------------------- I post from my phone. Excuse the typos and autocorrects.
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,811
Loc: Canada
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Re: Part of jar not colonizing [Re: sytar]
#19119425 - 11/11/13 08:32 AM (10 years, 3 months ago) |
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Quote:
sytar said: Looked fine to me, just monokaryotic mycelial growth stalling near the bottom. Smell it when you birth but it should be fine IMO (though I am a newb).
If OP has an entire cake of monokaryotic mycelium, which if he used ms is less likely than me winning the damn lottery, then it would be pretty much useless to him, as it would not be able to fruit. At all. My money is on bacteria for sure. That melted ice cream appearance close to the uncolonized portion screams bacteria IMO. I ain't much of a cake guy so maybe its different with cakes but, if I saw something like that in one of my grain jars it would be in the trash.
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Wydue Wanano
MUH SCIENCE



Registered: 09/02/13
Posts: 37
Last seen: 8 years, 9 months
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I have had this exact thing happen and I can tell you definitively that it is bacteria.
1) Mycelium near the spot is very dense, enveloping the spot 2) Mycelium a little further away has a darkish yellow tinge 3) In the spot there is what appears to be dark yellow gunk 4) If you remove this spot and smell it, it has a very distinct and unpleasant one
I cultured some bacteria from my current batch of failures and actually saw them under microscope. As said above, if you clean it off it can still fruit, but is a huge contam risk if you plan to acquire any sterile spores etc. from this batch.
-------------------- W'sup, I'm a fourth year chem major. Got chemistry questions? Ask me! I know the most about physical and organic chemistry.
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