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OfflineTehMyceliumMonster
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mixing grain & sub before inoculation?
    #19118393 - 11/11/13 12:49 AM (10 years, 2 months ago)

I did an invitro grow a while ago & had limited success (not the highest yeilds but 10+ flushes per bag, after the first flush they were dunked & put in an fc).
Id much rather do a bulk grow in a monotub but my house for whatever reason is prone to contams so invitro is all i can really do at the moment.

A few months after i did that grow i realized that the bags i used had a mix of grain & some kind of sub. This kind of threw me off because its not how things are traditionally done & i havnt had enough experience to really deviate from the book much.
Could the grain/sub mix before inoculation cause any problems?(ex. Weak myc, stalling, potency drop, contams, early pinning, etc.)


Edited by TehMyceliumMonster (11/11/13 01:40 AM)


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OfflinePussyFart
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Re: mixing spawn & sub before inoculation? [Re: TehMyceliumMonster]
    #19118429 - 11/11/13 12:58 AM (10 years, 2 months ago)

Quote:

TehMyceliumMonster said:
Could the spawn/sub mix before inoculation cause any problems?(ex. Weak myc, stalling, potency drop, contams, early pinning, etc.)



This is confusing.....

Mixing spawn with the substrate IS considered inoculation.....


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InvisibleLiquidGlass
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Re: mixing spawn & sub before inoculation? [Re: PussyFart]
    #19118473 - 11/11/13 01:14 AM (10 years, 2 months ago)

:whathesaid:


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OfflineTehMyceliumMonster
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Re: mixing spawn & sub before inoculation? [Re: PussyFart]
    #19118486 - 11/11/13 01:17 AM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Quote:

TehMyceliumMonster said:
Could the spawn/sub mix before inoculation cause any problems?(ex. Weak myc, stalling, potency drop, contams, early pinning, etc.)



This is confusing.....

Mixing spawn with the substrate IS considered inoculation.....




The spawn doesnt have any mycelium on it, the bags come with a mix of substrate & spawn, you have to use ms or lc to get spores in there.
It confused me because usually youd colonize the grain first & then mix with sub & recolonize then fruit.


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InvisibleLiquidGlass
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Re: mixing spawn & sub before inoculation? [Re: TehMyceliumMonster]
    #19118491 - 11/11/13 01:18 AM (10 years, 2 months ago)

Uncolonized grain is not spawn, it only becomes spawn when it is colonized with mycelium

And no, you cannot mix them before inoculation because grains need to be sterilized while substrate needs to be pasteurized


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I was raised a christian and was a stone-faced acid head
- Ken Kesey


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InvisibleLiquidGlass
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Re: mixing spawn & sub before inoculation? [Re: LiquidGlass]
    #19118501 - 11/11/13 01:21 AM (10 years, 2 months ago)

My house is very contam prone as well. With how much rain we get we are considered a rain forest. It is so moist here that mold often grows on peoples walls just because.

But as long as your sterile technique is good you can knock up some grain and get it going.


There are always going to be mold spores in the air no matter what you do


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I was raised a christian and was a stone-faced acid head
- Ken Kesey


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OfflineTehMyceliumMonster
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Re: mixing spawn & sub before inoculation? [Re: LiquidGlass]
    #19118512 - 11/11/13 01:25 AM (10 years, 2 months ago)

Quote:

LiquidGlass said:
Uncolonized grain is not spawn, it only becomes spawn when it is colonized with mycelium

And no, you cannot mix them before inoculation because grains need to be sterilized while substrate needs to be pasteurized




I did not physically mix & sterilize them myself. I bought a a pre sterilized, sealed, filter patched,bag with mix of substrate & what looks like rye berries & wbs inside.


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InvisibleLiquidGlass
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Re: mixing spawn & sub before inoculation? [Re: TehMyceliumMonster]
    #19118538 - 11/11/13 01:31 AM (10 years, 2 months ago)

Quote:

TehMyceliumMonster said:
Quote:

LiquidGlass said:
Uncolonized grain is not spawn, it only becomes spawn when it is colonized with mycelium

And no, you cannot mix them before inoculation because grains need to be sterilized while substrate needs to be pasteurized




I did not physically mix & sterilize them myself. I bought a a pre sterilized, sealed, filter patched,bag with mix of substrate & what looks like rye berries & wbs inside.





I realize that but you keep calling it spawn and if you do not want to confuse people when you ask for help then you need to get your terms right


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I was raised a christian and was a stone-faced acid head
- Ken Kesey


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InvisibleLiquidGlass
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Re: mixing spawn & sub before inoculation? [Re: LiquidGlass]
    #19118550 - 11/11/13 01:33 AM (10 years, 2 months ago)

Quote:

Could the spawn/sub mix before inoculation cause any problems?(ex. Weak myc, stalling, potency drop, contams, early pinning, etc.)




I though you were asking if you could mix the spawn and sub before inoculation


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Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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OfflineTehMyceliumMonster
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Re: mixing spawn & sub before inoculation? [Re: LiquidGlass]
    #19118566 - 11/11/13 01:37 AM (10 years, 2 months ago)

Quote:

LiquidGlass said:
Quote:

TehMyceliumMonster said:
Quote:

LiquidGlass said:
Uncolonized grain is not spawn, it only becomes spawn when it is colonized with mycelium

And no, you cannot mix them before inoculation because grains need to be sterilized while substrate needs to be pasteurized




I did not physically mix & sterilize them myself. I bought a a pre sterilized, sealed, filter patched,bag with mix of substrate & what looks like rye berries & wbs inside.





I realize that but you keep calling it spawn and if you do not want to confuse people when you ask for help then you need to get your terms right




My bad lol. I read that post & took it as you thought i was trying to mix myself.

Im just confused as to why an invitro bag would have both grain & sub in it.


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InvisibleLiquidGlass
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Re: mixing spawn & sub before inoculation? [Re: TehMyceliumMonster]
    #19118583 - 11/11/13 01:41 AM (10 years, 2 months ago)

They are probably both sterilized but since you do not have to exposed either of them to air when you inoculate they can be in the same bag. Pasteurizing leaves some microorganisms that help fight against contam. They probably pre mix them for ease of use, practically foolproof colonization


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Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Re: mixing spawn & sub before inoculation? [Re: LiquidGlass]
    #19118587 - 11/11/13 01:42 AM (10 years, 2 months ago)

But regardless, you could pull off bulk even in a contam prone zone


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I was raised a christian and was a stone-faced acid head
- Ken Kesey


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OfflineTehMyceliumMonster
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Re: mixing spawn & sub before inoculation? [Re: LiquidGlass]
    #19118664 - 11/11/13 01:58 AM (10 years, 2 months ago)

Okay i got you. I was worried that not colonizing grain first then moving to bulk would somehow mess with myc growth or potency or something.

& as for contams im not so much worried about the grain colonizing.
Id have to take the spawn out of the jars to get it into the substrate & between the open air hitting the myc & the second colonization im worried itll mold before it fruits.

When it all comes down to it though its not really the contams that gets to me its the thought of investing money in a grow & not getting anything out of it.


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OfflinePussyFart
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Re: mixing spawn & sub before inoculation? [Re: TehMyceliumMonster]
    #19118676 - 11/11/13 02:01 AM (10 years, 2 months ago)

Quote:

TehMyceliumMonster said:
Id have to take the spawn out of the jars to get it into the substrate & between the open air hitting the myc & the second colonization im worried itll mold before it fruits.



Everyone goes thru this....people rarely spawn their tubs in sterile environments.

Pasteurized bulk substrate material, along with fully colonized spawn, is contam resistant.

THis is how we get away with spawning in open, dirty air.


Edited by PussyFart (11/11/13 02:01 AM)


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InvisibleLiquidGlass
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Re: mixing spawn & sub before inoculation? [Re: TehMyceliumMonster]
    #19118689 - 11/11/13 02:03 AM (10 years, 2 months ago)

As long as your spawn is clean when you mix it with the sub it will colonize just fine, esp. if you just use verm/coco fiber mix. You would need a pressure cooker if you do not have one. They myc somewhat of what you could call an immune system that will fight off contams before it gets too old.


--------------------
Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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