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mrnixon
noob


Registered: 10/14/13
Posts: 20
Last seen: 9 years, 11 months
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spore bank that has to remain unamed do to forum rules
#19118386 - 11/11/13 12:46 AM (10 years, 2 months ago) |
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This is my review. Im a noob to mushroom growing and for my first spore purchase I decided to go with 'unnamed spore bank'. I looked around on line at other spore banks but decided to go with 'unnamed spore bank' because their prices were much better than everywhere else, also their site was very proffesional and convincing looking, and so I bought a 4 pack of there supercharged syringes, PF classics. I paid $25 for them. I used PFtek, as most noobs here do, and did everything by the book and inoculated 12 jars using all 4 syringes. After about a week mycillium started to grow, and in some of the jars started to grow some black and grey stuff. After about two weeks all jars stared to show mycilium and contaminates. Its been almost 4 weeks since I inoculated the jars and all of the jars eventually had contaminates growing along side the mycillium. The mycillium and contaminates seem to be competing with each other in that the mycillium will not grow over the contaminates, only in places that are contaminate free. Its a total loss, all the jars are so full of contaminates that its seems useless to go ahead and try and fruit them. The contaminates are grey, black, green, purple and bright pink which to me seem almost toxic. As much as I wanted this to be a success and tried to convince myself that they could still be salvaged, in the end it was just a waste of time and money. this was for my microscopy puposes.
Edited by mrnixon (11/11/13 01:03 AM)
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Re: Sporestore [Re: mrnixon]
#19118394 - 11/11/13 12:49 AM (10 years, 2 months ago) |
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3. Spores are sold for microscopy use only so mycologists can ID mushrooms. If you're going to misuse them to grow a controlled substance, keep it to yourself. Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning. Do not plug non sponsors.
Please edit your post and remove the vendors name, then read the forum rules.
Rules Thread-READ BEFORE POSTING
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
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Maybe, instead of blaming the vendor for your failure, you should blame your own actions...
Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...
You only need to use a few drops per hole, per jar....by stretching the spores, you also stretch the chance of grabbing a contam out of the syringe....every drop will be different.
How was your sterile technique?
Walk us through everything you did, from the start.
You said you did everything by the book, but that went out the window when you used 3ccs per jar.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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TheWitchfinder


Registered: 08/28/13
Posts: 76
Loc: Salem
Last seen: 3 years, 4 months
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Quote:
Notahacker420 said:
3. Spores are sold for microscopy use only so mycologists can ID mushrooms. If you're going to misuse them to grow a controlled substance, keep it to yourself. Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning. Do not plug non sponsors.
Please edit your post and remove the vendors name, then read the forum rules.
Rules Thread-READ BEFORE POSTING
Please please please. For a lot of reasons listen to what Notahacker is saying. There are many reasons why you should NEVER mention a vendors name.
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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Quote:
inoculated 12 jars using all 4 syringes
That is not by the book at all
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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mrnixon
noob


Registered: 10/14/13
Posts: 20
Last seen: 9 years, 11 months
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Re: spore bank that has to remain unamed do to forum rules [Re: mrnixon]
#19118498 - 11/11/13 01:19 AM (10 years, 2 months ago) |
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The PFtek 'lets grow mushrooms' videos here on this site doesn't say how much to use for inoculation so I used about 4 ml per 1/2 quart jar. Maybe that was to much. just a few drops per hole you say, maybe I shoulda done that. As for my procedure it was steaming the jars for 90 minutes then flame sterilize the needle and without blowing on it and immediately insert it into the jar. The only thing I didn't use is a glovebox.
-------------------- can anyone get me some Lsd
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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Re: spore bank that has to remain unamed do to forum rules [Re: mrnixon]
#19118525 - 11/11/13 01:28 AM (10 years, 2 months ago) |
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Quote:
mrnixon said: The PFtek 'lets grow mushrooms' videos here on this site doesn't say how much to use for inoculation so I used about 4 ml per 1/2 quart jar. Maybe that was to much. just a few drops per hole you say, maybe I shoulda done that. As for my procedure it was steaming the jars for 90 minutes then flame sterilize the needle and without blowing on it and immediately insert it into the jar. The only thing I didn't use is a glovebox.
I have not watched all of the videos. The people who made those however, videos did not come up with the pf tek. That was Psilocybe Fanaticus aka Robert Mcpherson. In his book it stated to use 1cc per jar here is the link if you are interested
http://www.fanaticus.com/
The first time I ever ordered spores was straight from this guy, he is no longer with us
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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ghostshadow422
Stranger


Registered: 10/02/13
Posts: 162
Last seen: 8 years, 17 days
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Re: spore bank that has to remain unamed do to forum rules [Re: LiquidGlass]
#19118613 - 11/11/13 01:48 AM (10 years, 2 months ago) |
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I plugged 5 CCs of liquid culture per jar, is that too much then? oh yeah and I only sterilize once every 2 jars ( 1 full 10cc syringe)
Edited by ghostshadow422 (11/11/13 01:49 AM)
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OgreLokon
Pretty Fun Guy


Registered: 06/30/13
Posts: 512
Last seen: 6 years, 5 months
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Re: spore bank that has to remain unamed do to forum rules [Re: ghostshadow422]
#19118619 - 11/11/13 01:48 AM (10 years, 2 months ago) |
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Sterilize every jar
Edited by OgreLokon (11/11/13 01:49 AM)
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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Re: spore bank that has to remain unamed do to forum rules [Re: OgreLokon]
#19118643 - 11/11/13 01:52 AM (10 years, 2 months ago) |
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Way too much, 1 cc per jar
Back when I used to do pf tek I only sterilized the needle once before inoculation and that was it. Worked for me . . .
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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ghostshadow422
Stranger


Registered: 10/02/13
Posts: 162
Last seen: 8 years, 17 days
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Re: spore bank that has to remain unamed do to forum rules [Re: LiquidGlass]
#19118677 - 11/11/13 02:01 AM (10 years, 2 months ago) |
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I am currently birthing 73 cakes and only two had contams
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
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Re: spore bank that has to remain unamed do to forum rules [Re: ghostshadow422]
#19118687 - 11/11/13 02:03 AM (10 years, 2 months ago) |
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Quote:
ghostshadow422 said: I am currently birthing 73 cakes and only two had contams
This does not mean sub-standard sterile techniques will always work.
Better safe than sorry....flame between every jar.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Enigma1
Positive



Registered: 08/15/13
Posts: 977
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Re: spore bank that has to remain unamed do to forum rules [Re: PussyFart]
#19121424 - 11/11/13 03:59 PM (10 years, 2 months ago) |
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Buy another oyster syringe and try again .
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Gymspawn
Stranger
Registered: 11/10/13
Posts: 100
Last seen: 8 years, 8 days
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Re: spore bank that has to remain unamed do to forum rules [Re: Enigma1]
#19121501 - 11/11/13 04:10 PM (10 years, 2 months ago) |
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half quart jars not by the book in PF tek and I agree way to much solution leads too much room for contams.
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justarandom
around here somewhere

Registered: 07/28/13
Posts: 67
Last seen: 1 year, 2 months
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Re: spore bank that has to remain unamed do to forum rules [Re: mrnixon]
#19121673 - 11/11/13 04:39 PM (10 years, 2 months ago) |
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Quote:
mrnixon said: This is my review. Im a noob to mushroom growing and for my first spore purchase I decided to go with 'unnamed spore bank'. I looked around on line at other spore banks but decided to go with 'unnamed spore bank' because their prices were much better than everywhere else, also their site was very proffesional and convincing looking, and so I bought a 4 pack of there supercharged syringes, PF classics. I paid $25 for them. I used PFtek, as most noobs here do, and did everything by the book and inoculated 12 jars using all 4 syringes. After about a week mycillium started to grow, and in some of the jars started to grow some black and grey stuff. After about two weeks all jars stared to show mycilium and contaminates. Its been almost 4 weeks since I inoculated the jars and all of the jars eventually had contaminates growing along side the mycillium. The mycillium and contaminates seem to be competing with each other in that the mycillium will not grow over the contaminates, only in places that are contaminate free. Its a total loss, all the jars are so full of contaminates that its seems useless to go ahead and try and fruit them. The contaminates are grey, black, green, purple and bright pink which to me seem almost toxic. As much as I wanted this to be a success and tried to convince myself that they could still be salvaged, in the end it was just a waste of time and money. this was for my microscopy puposes.
i got 6 syringes for 35 which is cheaper than your 4 for 25 =? and it was threw a shroomery sponsor look harder next time please and support your community =)
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
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Quote:
Notahacker420 said: Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...
Wetness doesn't cause contamination. It can cause the mycelium to stall long enough for a bacterial endospore that was merely damaged by steam sterilization to recover, but obviously that's not the case here because OP didn't observe stalling and the contaminants sound fungal in nature.
OP, did your contaminations originate in the same spot as your healthy mycelium, or was it ever somewhere else (like the bottom of the jar or halfway between inoculation points)?
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
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Quote:
van der griegen said:
Quote:
Notahacker420 said: Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...
Wetness doesn't cause contamination.
I never said it did....
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Psilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
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Quote:
Notahacker420 said:
Quote:
van der griegen said:
Quote:
Notahacker420 said: Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...
Wetness doesn't cause contamination.
I never said it did....
Sorry, I must have misunderstood when you said OP should blame his own actions for his contamination, like using four syringes to knock up a dozen jars.
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Gymspawn
Stranger
Registered: 11/10/13
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Last seen: 8 years, 8 days
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Re: spore bank that has to remain unamed do to forum rules [Re: Enigma1]
#19121950 - 11/11/13 05:32 PM (10 years, 2 months ago) |
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Yea if you keep going the way you are you will eventually have some issues that you could of bypassed by taking the proper steps.
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Gymspawn
Stranger
Registered: 11/10/13
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Re: spore bank that has to remain unamed do to forum rules [Re: Gymspawn]
#19121959 - 11/11/13 05:34 PM (10 years, 2 months ago) |
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I believe what he's saying is that no syringe or very rarely a syringe is contam free but the more solution you use the more chances you have for using the sealed in contam possibility's.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
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Quote:
van der griegen said:
Quote:
Notahacker420 said:
Quote:
van der griegen said:
Quote:
Notahacker420 said: Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...
Wetness doesn't cause contamination.
I never said it did....
Sorry, I must have misunderstood when you said OP should blame his own actions for his contamination, like using four syringes to knock up a dozen jars.
Well, ok...that does make sense when you look at it like that, but look at what I said.
I said his failures came from using too much spore solution, not contamination.
I was not referring to what he said, but what I was talking about in the line above.
Even you said too much moisture could cause stalling.....well in my mind that is a failure.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
Edited by PussyFart (11/11/13 07:07 PM)
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mrnixon
noob


Registered: 10/14/13
Posts: 20
Last seen: 9 years, 11 months
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The contaminates started at the mycelium but after a few weeks contaminates started at other areas away from mycelium.
-------------------- can anyone get me some Lsd
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Psilicon
Really Nice Guy


Registered: 08/26/12
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Re: Sporestore [Re: mrnixon]
#19124138 - 11/11/13 11:48 PM (10 years, 2 months ago) |
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It sounds like your sterilization procedure needs some work, then.
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Sporestore [Re: mrnixon]
#19124139 - 11/11/13 11:48 PM (10 years, 2 months ago) |
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First time I tried I knocked up like 6 jars out of 20... You'll probably fail and make mistakes... But you can learn from them... It's not as easy as it looks in the videos... Yes it can be I'm sure, but not when it is new territory that you don't yet have a deep perception and understanding of.
Use this opportunity to learn... And all the jars you describe, I would not even try to save them for future use, don't seem like you have a PC, the jars might be contamed for good... Unless you really make an effort but as you may have seen, even breathing air from a contamed jar can be dangerous.
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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spacechildo
proletarians rise up



Registered: 01/24/13
Posts: 19,243
Loc: Babylon
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Quote:
van der griegen said:
Quote:
Notahacker420 said: Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...
Wetness doesn't cause contamination.
but it sure gives bacteria a great environment to thrive in! my towel doesn't grow mold by itself, but if I keep it wet and tucked in a plastic bag it will start to smell and mold.
the moisture sure didn't create the contams, but gave it parameters to grow in.
Also op, it sounded kinda funny when you said the site seems professional, and then they sold you "supercharge syringe"
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Psilicon
Really Nice Guy


Registered: 08/26/12
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Quote:
spacechildo said: my towel doesn't grow mold by itself, but if I keep it wet and tucked in a plastic bag it will start to smell and mold.
If you put your towel into a jar, sealed it and then pressure cooked it for an hour, I'd be very surprised if it started to smell.
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LiquidGlass
Glass Blower


Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
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Quote:
van der griegen said:
Quote:
spacechildo said: my towel doesn't grow mold by itself, but if I keep it wet and tucked in a plastic bag it will start to smell and mold.
If you put your towel into a jar, sealed it and then pressure cooked it for an hour, I'd be very surprised if it started to smell.
Quote:
van der griegen said:
Quote:
spacechildo said: my towel doesn't grow mold by itself, but if I keep it wet and tucked in a plastic bag it will start to smell and mold.
If you put your towel into a jar, sealed it and then pressure cooked it for an hour, I'd be very surprised if it started to smell.
There are bacterial endospores that can actually survive the pressure cooker. And if there is no mycelium there to prevent it from germinating, it can eventually start to grow and contaminate. Th the towel would probably smell eventually . . .
-------------------- Some art I've made Glass Art Gallery
  I was raised a christian and was a stone-faced acid head - Ken Kesey
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