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Offlinemrnixon
noob


Registered: 10/14/13
Posts: 20
Last seen: 9 years, 11 months
spore bank that has to remain unamed do to forum rules
    #19118386 - 11/11/13 12:46 AM (10 years, 2 months ago)

This is my review. Im a noob to mushroom growing and for my first spore purchase I decided to go with 'unnamed spore bank'.  I looked around on line at other spore banks but decided to go with 'unnamed spore bank' because their prices were much better than everywhere else, also their site was very proffesional and convincing looking, and so I bought a 4 pack of there supercharged syringes, PF classics.  I paid $25 for them.  I used PFtek, as most noobs here do, and did everything by the book and inoculated 12 jars using all 4 syringes.  After about a week mycillium started to grow, and in some of the jars started to grow some black and grey stuff.  After about two weeks all jars stared to show mycilium and contaminates. Its been almost 4 weeks since I inoculated the jars and all of the jars eventually had contaminates growing along side the mycillium. The mycillium and contaminates seem to be competing with each other in that the mycillium will not grow over the contaminates, only in places that are contaminate free.  Its a total loss, all the jars are so full of contaminates that its seems useless to go ahead and try and fruit them.  The contaminates are grey, black, green, purple and bright pink which to me seem almost toxic. 
As much as I wanted this to be a success and tried to convince myself that they could still be salvaged, in the end it was just a waste of time and money.
this was for my microscopy puposes.


Edited by mrnixon (11/11/13 01:03 AM)


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
Trusted Cultivator
Re: Sporestore [Re: mrnixon]
    #19118394 - 11/11/13 12:49 AM (10 years, 2 months ago)

:smbfacepalm:    :rules:

3.  Spores are sold for microscopy use only so mycologists can ID mushrooms. If you're going to misuse them to grow a controlled substance, keep it to yourself.  Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning.  Do not plug non sponsors.

Please edit your post and remove the vendors name, then read the forum rules.

Rules Thread-READ BEFORE POSTING


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
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Re: Sporestore [Re: PussyFart]
    #19118416 - 11/11/13 12:54 AM (10 years, 2 months ago)

Maybe, instead of blaming the vendor for your failure, you should blame your own actions...

Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...

You only need to use a few drops per hole, per jar....by stretching the spores, you also stretch the chance of grabbing a contam out of the syringe....every drop will be different.

How was your sterile technique?

Walk us through everything you did, from the start.

You said you did everything by the book, but that went out the window when you used 3ccs per jar.


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OfflineTheWitchfinder


Registered: 08/28/13
Posts: 76
Loc: Salem
Last seen: 3 years, 4 months
Re: Sporestore [Re: PussyFart]
    #19118418 - 11/11/13 12:55 AM (10 years, 2 months ago)

Quote:

Notahacker420 said:
:smbfacepalm:    :rules:

3.  Spores are sold for microscopy use only so mycologists can ID mushrooms. If you're going to misuse them to grow a controlled substance, keep it to yourself.  Any post which mentions 'such and such' vendor, will be deleted and you'll receive an official warning.  Do not plug non sponsors.

Please edit your post and remove the vendors name, then read the forum rules.

Rules Thread-READ BEFORE POSTING





Please please please. For a lot of reasons listen to what Notahacker is saying. There are many reasons why you should NEVER mention a vendors name.


--------------------
TheWitchfinder's LAGM2020 Log


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InvisibleLiquidGlass
Glass Blower
 User Gallery

Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
Re: Sporestore [Re: TheWitchfinder]
    #19118482 - 11/11/13 01:16 AM (10 years, 2 months ago)

Quote:

inoculated 12 jars using all 4 syringes




That is not by the book at all


--------------------
Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Offlinemrnixon
noob


Registered: 10/14/13
Posts: 20
Last seen: 9 years, 11 months
Re: spore bank that has to remain unamed do to forum rules [Re: mrnixon]
    #19118498 - 11/11/13 01:19 AM (10 years, 2 months ago)

The PFtek 'lets grow mushrooms' videos here on this site doesn't say how much to use for inoculation so I used about 4 ml per 1/2 quart jar.  Maybe that was to much.  just a few drops per hole you say, maybe I shoulda done that. 
As for my procedure it was steaming the jars for 90 minutes then flame sterilize the needle and without blowing on it and immediately insert it into the jar.  The only thing I didn't use is a glovebox.


--------------------
can anyone get me some Lsd


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InvisibleLiquidGlass
Glass Blower
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Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
Re: spore bank that has to remain unamed do to forum rules [Re: mrnixon]
    #19118525 - 11/11/13 01:28 AM (10 years, 2 months ago)

Quote:

mrnixon said:
The PFtek 'lets grow mushrooms' videos here on this site doesn't say how much to use for inoculation so I used about 4 ml per 1/2 quart jar.  Maybe that was to much.  just a few drops per hole you say, maybe I shoulda done that. 
As for my procedure it was steaming the jars for 90 minutes then flame sterilize the needle and without blowing on it and immediately insert it into the jar.  The only thing I didn't use is a glovebox.




I have not watched all of the videos.  The people who made those however, videos did not come up with the pf tek. That was Psilocybe Fanaticus aka Robert Mcpherson. In his book it stated to use 1cc per jar here is the link if you are interested

http://www.fanaticus.com/

The first time I ever ordered spores was straight from this guy, he is no longer with us


--------------------
Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Offlineghostshadow422
Stranger
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Registered: 10/02/13
Posts: 162
Last seen: 8 years, 17 days
Re: spore bank that has to remain unamed do to forum rules [Re: LiquidGlass]
    #19118613 - 11/11/13 01:48 AM (10 years, 2 months ago)

I plugged 5 CCs of liquid culture per jar, is that too much then? oh yeah and I only sterilize once every 2 jars ( 1 full 10cc syringe)


Edited by ghostshadow422 (11/11/13 01:49 AM)


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OfflineOgreLokon
Pretty Fun Guy
Male

Registered: 06/30/13
Posts: 512
Last seen: 6 years, 5 months
Re: spore bank that has to remain unamed do to forum rules [Re: ghostshadow422]
    #19118619 - 11/11/13 01:48 AM (10 years, 2 months ago)

Sterilize every jar


Edited by OgreLokon (11/11/13 01:49 AM)


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InvisibleLiquidGlass
Glass Blower
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Registered: 07/08/12
Posts: 5,288
Loc: Pee En Double You
Re: spore bank that has to remain unamed do to forum rules [Re: OgreLokon]
    #19118643 - 11/11/13 01:52 AM (10 years, 2 months ago)

Way too much, 1 cc per jar

Back when I used to do pf tek I only sterilized the needle once before inoculation and that was it. Worked for me . . .


--------------------
Some art I've made Glass Art Gallery

I was raised a christian and was a stone-faced acid head
- Ken Kesey


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Offlineghostshadow422
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Registered: 10/02/13
Posts: 162
Last seen: 8 years, 17 days
Re: spore bank that has to remain unamed do to forum rules [Re: LiquidGlass]
    #19118677 - 11/11/13 02:01 AM (10 years, 2 months ago)

I am currently birthing 73 cakes and only two had contams


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
Trusted Cultivator
Re: spore bank that has to remain unamed do to forum rules [Re: ghostshadow422]
    #19118687 - 11/11/13 02:03 AM (10 years, 2 months ago)

Quote:

ghostshadow422 said:
I am currently birthing 73 cakes and only two had contams



This does not mean sub-standard sterile techniques will always work.

Better safe than sorry....flame between every jar.


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InvisibleEnigma1
Positive
Male User Gallery


Registered: 08/15/13
Posts: 977
Re: spore bank that has to remain unamed do to forum rules [Re: PussyFart]
    #19121424 - 11/11/13 03:59 PM (10 years, 2 months ago)

Buy another oyster syringe and try again .


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OfflineGymspawn
Stranger
Registered: 11/10/13
Posts: 100
Last seen: 8 years, 8 days
Re: spore bank that has to remain unamed do to forum rules [Re: Enigma1]
    #19121501 - 11/11/13 04:10 PM (10 years, 2 months ago)

half quart jars not by the book in PF tek and I agree way to much solution leads too much room for contams.


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Offlinejustarandom
around here somewhere

Registered: 07/28/13
Posts: 67
Last seen: 1 year, 2 months
Re: spore bank that has to remain unamed do to forum rules [Re: mrnixon]
    #19121673 - 11/11/13 04:39 PM (10 years, 2 months ago)

Quote:

mrnixon said:
This is my review. Im a noob to mushroom growing and for my first spore purchase I decided to go with 'unnamed spore bank'.  I looked around on line at other spore banks but decided to go with 'unnamed spore bank' because their prices were much better than everywhere else, also their site was very proffesional and convincing looking, and so I bought a 4 pack of there supercharged syringes, PF classics.  I paid $25 for them.  I used PFtek, as most noobs here do, and did everything by the book and inoculated 12 jars using all 4 syringes.  After about a week mycillium started to grow, and in some of the jars started to grow some black and grey stuff.  After about two weeks all jars stared to show mycilium and contaminates. Its been almost 4 weeks since I inoculated the jars and all of the jars eventually had contaminates growing along side the mycillium. The mycillium and contaminates seem to be competing with each other in that the mycillium will not grow over the contaminates, only in places that are contaminate free.  Its a total loss, all the jars are so full of contaminates that its seems useless to go ahead and try and fruit them.  The contaminates are grey, black, green, purple and bright pink which to me seem almost toxic. 
As much as I wanted this to be a success and tried to convince myself that they could still be salvaged, in the end it was just a waste of time and money.
this was for my microscopy puposes.




i got 6 syringes for 35 which is cheaper than your 4 for 25 =? and it was threw a shroomery sponsor look harder next time please and support your community =)


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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
Re: Sporestore [Re: PussyFart]
    #19121892 - 11/11/13 05:21 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...




Wetness doesn't cause contamination.  It can cause the mycelium to stall long enough for a bacterial endospore that was merely damaged by steam sterilization to recover, but obviously that's not the case here because OP didn't observe stalling and the contaminants sound fungal in nature.

OP, did your contaminations originate in the same spot as your healthy mycelium, or was it ever somewhere else (like the bottom of the jar or halfway between inoculation points)?


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OfflinePussyFart
Retired Cultivation Extrodinaire
Male


Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 15 hours
Trusted Cultivator
Re: Sporestore [Re: Psilicon]
    #19121910 - 11/11/13 05:24 PM (10 years, 2 months ago)

Quote:

van der griegen said:
Quote:

Notahacker420 said:
Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...




Wetness doesn't cause contamination.



I never said it did....


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OfflinePsilicon
Really Nice Guy


Registered: 08/26/12
Posts: 7,057
Last seen: 3 years, 1 month
Re: Sporestore [Re: PussyFart]
    #19121941 - 11/11/13 05:31 PM (10 years, 2 months ago)

Quote:

Notahacker420 said:
Quote:

van der griegen said:
Quote:

Notahacker420 said:
Like using 4 whole syringes to knock up only 12 jars.....could have been too wet...




Wetness doesn't cause contamination.



I never said it did....




Sorry, I must have misunderstood when you said OP should blame his own actions for his contamination, like using four syringes to knock up a dozen jars.


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OfflineGymspawn
Stranger
Registered: 11/10/13
Posts: 100
Last seen: 8 years, 8 days
Re: spore bank that has to remain unamed do to forum rules [Re: Enigma1]
    #19121950 - 11/11/13 05:32 PM (10 years, 2 months ago)

Yea if you keep going the way you are you will eventually have some issues that you could of bypassed by taking the proper steps.


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OfflineGymspawn
Stranger
Registered: 11/10/13
Posts: 100
Last seen: 8 years, 8 days
Re: spore bank that has to remain unamed do to forum rules [Re: Gymspawn]
    #19121959 - 11/11/13 05:34 PM (10 years, 2 months ago)

I believe what he's saying is that no syringe or very rarely a syringe is contam free but the more solution you use the more chances you have for using the sealed in contam possibility's.


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