Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: North Spore Cultivation Supplies   Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
InvisibleRuralAnomaly
Sporadic
Male User Gallery

Registered: 10/05/13
Posts: 2,153
Loc: Spitzenkörper Ohio
measuring spores without a reticule
    #19114114 - 11/10/13 10:18 AM (10 years, 2 months ago)

i've been wracking my brain for a few days (not a pretty thing if you're anywhere close)

primarily, how to compensate for less than ideal equipment microscopically, and still get useful information, esp. regarding spore dimensions.

here's my chain of thinking so far.

I got wind of piximeter from one of Alan's posts and i can muddle through the french a bit and even luckier, cuz i'm rusty, the program installs in english.  so that's installed.

now to the part i'd like discussed:
since some of the images you can find show the dimensions AND a reference bar ( |---------| equals 200 µm for example )

could you use THAT bar to create a reference against which other images that [i would] load into piximeter?
first load the above image and use that as the calibration reference
then, i image some spores from a macroscopically well id'd species and measure its spores (preferably even same species as above?)

its sort of going backwards, and may be convoluted and i know that largely the propagation of error is going to come from the variance in the spore size of the species i select to use as a means to calibrate MY scope against the literature (so perhaps ideally choosing one with as little variance in mature spores as possible to find)
AND i just started messing with the program so its subtle niceties are lost on me as of yet, but it seems to be a v. useful tool, if not this way then definitely down the line.

there may be some serious flaws in my logic here due to some factors i haven't considered (either out of flat out ignorance that they are factors, or just me being generally ignorant) but wouldn't it be wunnerful if technology gave us yet another work-around to compensate for no reticule

TIA for your thoughts on this

oh and please mention any other pixel measuring software you use (esp. free/shareware lol)


--------------------
bite my basidiocarp


Extras: Filter Print Post Top
InvisibleByrain

Registered: 01/07/10
Posts: 9,664
Trusted Identifier
Re: measuring spores without a reticule [Re: RuralAnomaly]
    #19114194 - 11/10/13 10:36 AM (10 years, 2 months ago)

You calibrate your scope with a stage micrometer because its a known size, the reticle in a eye piece and any images you find online wont necessarily conform to those sizes.


Extras: Filter Print Post Top
InvisibleRuralAnomaly
Sporadic
Male User Gallery

Registered: 10/05/13
Posts: 2,153
Loc: Spitzenkörper Ohio
Re: measuring spores without a reticule [Re: Byrain]
    #19114266 - 11/10/13 10:51 AM (10 years, 2 months ago)

i guess the idea behind it is solving for unknowns using algebra or ratios whichever it is

just using made up numbers here to flesh out my thinking:

from reference pic
if spore A = 10µm which in pixels=40  and its 'reference bar' of 100µm would then be 400 pixels (on my screen only i understand, i'm creating a measurement unique to my gear and might not be the same everywhere)

this i use as my calibration reference for piximeter

i generate my own picture of maybe preferably the same type spores. 

now, in my homemade picture,
those spores all measure 8µm using piximeter 'uncalibrated'.  therefore, i'm 20% off vs the referent and would need to calculate accordingly for future measurements on MY rig of computer/camera/scope OR possibly be able to create an alternative 'calibration' using piximeter for subsequent photos.

math makes my brain hurt lol

does this make sense?


--------------------
bite my basidiocarp


Edited by RuralAnomaly (11/10/13 10:52 AM)


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: North Spore Cultivation Supplies   Bridgetown Botanicals Bridgetown Botanicals   Mushroom-Hut Substrate Bags   Left Coast Kratom Buy Kratom Extract


Similar ThreadsPosterViewsRepliesLast post
* mushroom hunt results, now with spore prints + working pics em_pathy 5,643 12 06/27/03 03:18 PM
by Anonymous
* Spore Results: P. castaneifolius and subbalteatus PICS!
( 1 2 all )
Anonymous 4,644 27 09/04/02 01:26 PM
by Anonymous
* Are any black spored, gilled,dung shrooms poison? QuitDrinking 3,519 4 09/17/02 12:12 AM
by Anonymous
* Re: "NO-SPORE" and NO COWS Anonymous 4,106 6 12/27/99 08:24 PM
by Abaddon
* how to do a spore print? 311 3,418 8 04/11/02 10:41 PM
by 311
* White spore print? lucky420 4,896 5 09/19/05 07:30 PM
by jobot37
* Sterile spore print from wild fungi? nermski 1,816 9 06/19/03 08:46 AM
by Jackal
* spray spores wacked 1,253 7 02/06/02 06:11 PM
by gorgebush

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: ToxicMan, inski, Alan Rockefeller, Duggstar, TimmiT, Anglerfish, Tmethyl, Lucis, Doc9151, Land Trout
420 topic views. 4 members, 19 guests and 4 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.023 seconds spending 0.009 seconds on 14 queries.