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OfflineEarthBounded
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Doc 34's WBS X FH Simple coir
    #19112214 - 11/09/13 09:26 PM (10 years, 2 months ago)

My idea for this month is to get tubs going using Doc 34s WBS tek: http://www.shroomery.org/9030/Doc34-s-Wild-Bird-Seed-Tek

I have 16 syringes and prints of my own Burma strain so I am not going to do G2G and risk contam, I am just going to inoculate all the grain jars then after colonization I am going to spawn it to franks simple coir tek:
http://www.shroomery.org/forums/showflat.php/Number/17325780#17325780

Sound like a good idea? Any recommendations?
I believe this would produce quick yields.

EB


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Offlinetwistedty
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Re: Doc 34's WBS X FH Simple coir [Re: EarthBounded]
    #19112238 - 11/09/13 09:30 PM (10 years, 2 months ago)

both great teks and thats a shit ton of syringes.

good luck :thumbup:


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InvisibleSpitballJedi
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Re: Doc 34's WBS X FH Simple coir [Re: EarthBounded]
    #19112260 - 11/09/13 09:35 PM (10 years, 2 months ago)

Quote:

EarthBounded said:
I have 16 syringes and prints of my own Burma strain so I am not going to do G2G and risk contam,..




I don't follow. How does this reduce risk of contams? did the mushroom you took the print from grow in sterile conditions? was the print taken in sterile conditions?


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InvisiblePastywhyteMDiscord
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Re: Doc 34's WBS X FH Simple coir [Re: SpitballJedi]
    #19112373 - 11/09/13 10:01 PM (10 years, 2 months ago)

Quote:

SpitballJedi said:
Quote:

EarthBounded said:
I have 16 syringes and prints of my own Burma strain so I am not going to do G2G and risk contam,..




I don't follow. How does this reduce risk of contams? did the mushroom you took the print from grow in sterile conditions? was the print taken in sterile conditions?



:whathesaid:
The only contam I ever got G2G was in a recieving jar that was cracked and I never noticed until I saw bacteria forming all along the crack. Otherwise I find G2G a lot safer than any syringe.


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OfflineEarthBounded
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Re: Doc 34's WBS X FH Simple coir [Re: SpitballJedi]
    #19120043 - 11/11/13 11:20 AM (10 years, 2 months ago)

Quote:

SpitballJedi said:
Quote:

EarthBounded said:
I have 16 syringes and prints of my own Burma strain so I am not going to do G2G and risk contam,..




I don't follow. How does this reduce risk of contams? did the mushroom you took the print from grow in sterile conditions? was the print taken in sterile conditions?




The reason I personally feel this will reduce con tams ( and I would like your second opinion's but I don't have a flow hood, I could build a SAB like Transcendinglifes tek, and probably will in the future, but I have 16 syringes all Cubes, direct from a sponsor, so I would Like to just inoculate say 8 wbs jars instead of doing the transfer, I am still nube so I would like to start Monos with simple coir but I guess i just feel safer knocking up 6-8 WBS jars per tub with self sealing injector ports to kind of skip G2G until I get a 100% effective SAB built, or flow hood.

Any advice or comments are appreciated.

Thank You kindly
EB


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Offlinespacechildo
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Re: Doc 34's WBS X FH Simple coir [Re: EarthBounded]
    #19120387 - 11/11/13 12:38 PM (10 years, 2 months ago)

you should have a sab for inoculation as well.
g2g is king. syringes are boring and slow :thumbup:


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InvisiblePastywhyteMDiscord
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Re: Doc 34's WBS X FH Simple coir [Re: spacechildo]
    #19120466 - 11/11/13 12:56 PM (10 years, 2 months ago)

Well you should be able to do about 20-30 jars per syringe, so you will be using them 16 syringes for a while. But limiting genetics is usually a good thing, so what most people prefer to do is knock up 6 jars or so, then G2G the fastest/healthiest looking ones. Cuts down on how many strains you will have competing in your monotub and might result in better results.

A good SAB is a must for agar work, so you will eventually want to get one so you can get into that. I recommend to save a couple drops from each variety so that you can knock up plates for ms grows and clone from them.


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OfflineEarthBounded
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Re: Doc 34's WBS X FH Simple coir [Re: Pastywhyte]
    #19120644 - 11/11/13 01:32 PM (10 years, 2 months ago)

Quote:

Pastywhyte said:
Well you should be able to do about 20-30 jars per syringe, so you will be using them 16 syringes for a while. But limiting genetics is usually a good thing, so what most people prefer to do is knock up 6 jars or so, then G2G the fastest/healthiest looking ones. Cuts down on how many strains you will have competing in your monotub and might result in better results.

A good SAB is a must for agar work, so you will eventually want to get one so you can get into that. I recommend to save a couple drops from each variety so that you can knock up plates for ms grows and clone from them.





Good info and I have noted everything thank you!

Quote:

spacechildo said:
you should have a sab for inoculation as well.
g2g is king. syringes are boring and slow :thumbup:




I have a pretty poorly built one right now and limited time, I am planning on building one like Transcending life but this whole thread was for a quick project for this month, I suppose it is just a good time to get into G2G I suppose I could just get a solid SAB built and start now.


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Offlinespacechildo
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Re: Doc 34's WBS X FH Simple coir [Re: EarthBounded]
    #19124983 - 11/12/13 05:22 AM (10 years, 2 months ago)

poorly built sab? it's just holes in a box. or do you mean a small sab?
i have a small sab myself, and I hate working in it.


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