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Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures

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Offlinesilverstem
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agar to lc?
    #19107506 - 11/08/13 08:08 PM (10 years, 2 months ago)

i was wondering how i could go from agar to lc?

also does a lc require ge?


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OfflinebEelzeBosS
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Re: agar to lc? [Re: silverstem]
    #19107525 - 11/08/13 08:11 PM (10 years, 2 months ago)

Just drop a colonized agar wedge into your prepared LC jar in a SAB or in front of a flow hood and shake the hell out of it. LC doesn't require GE, but I would recommend a GE port as it becomes difficult to draw the LC up into the syringe once the jar builds up pressure inside. I'm probably wording this all wrong, Ive had a lot to drink.


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Offlinesilverstem
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Re: agar to lc? [Re: bEelzeBosS]
    #19107545 - 11/08/13 08:15 PM (10 years, 2 months ago)

well im gonna use silicone for the injection port. thanks.


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OfflinecronicrFacebook
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Re: agar to lc? [Re: silverstem]
    #19107555 - 11/08/13 08:17 PM (10 years, 2 months ago)

what species you planning on trying this with?


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OfflinebEelzeBosS
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Re: agar to lc? [Re: silverstem]
    #19107567 - 11/08/13 08:20 PM (10 years, 2 months ago)

Quote:

silverstem said:
well im gonna use silicone for the injection port. thanks.




That's fine, but I'd recommend a 2nd port with a synthetic filter disc or an EZ Felt disc covering it so your jar doesn't build up pressure. Its not completely necessary, but it makes things a lot easier when you decide to draw up your LC.

If you decide to just go with the injection port only you could depressurize the jar by sticking in a sterilized syringe with iso saturated cotton balls and no plunger in the chamber.


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Shop: North Spore Injection Grain Bag   Mushroom-Hut Liquid Cultures


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