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jpack666



Registered: 10/01/13
Posts: 484
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Wbr fractional sterilization?
#19106434 - 11/08/13 04:05 PM (10 years, 2 months ago) |
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Hi all 
I've seen teks for fractional sterilization for about every possible grain except wbr?
Anyone has every tried something like that? Brown rice grain without a PC?
Thanks
Jpack
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Re: Wbr fractional sterilization? [Re: jpack666]
#19106452 - 11/08/13 04:10 PM (10 years, 2 months ago) |
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Fractional sterilization fails 90% of the time.
If you cannot PC grains, steam them for about 8 hours.
That being said, not sure about whole rice...should be able to be just steamed.
BRF can just be steamed, but I dunno.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say...
Last seen: 18 hours, 56 minutes
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Re: Wbr fractional sterilization? [Re: PussyFart]
#19106478 - 11/08/13 04:15 PM (10 years, 2 months ago) |
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Get a job, save some money, and buy a pc
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Wbr fractional sterilization? [Re: PussyFart]
#19106564 - 11/08/13 04:33 PM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: Fractional sterilization fails 90% of the time.
If you cannot PC grains, steam them for about 8 hours.
That being said, not sure about whole rice...should be able to be just steamed.
BRF can just be steamed, but I dunno.
They fail 90% of the time... That's good to know.... Thanks for your help, I'm thinking about what you said and it's helping me in my decision to buy a PC...
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Wbr fractional sterilization? [Re: Stromrider]
#19106901 - 11/08/13 05:41 PM (10 years, 2 months ago) |
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Quote:
Stromrider said: Get a job, save some money, and buy a pc 
Rough... But still... I asked and you answered. Pointing out the obviousness of my financial difficulties and impulsivity.
Best advice for my monkey brain
Thanks
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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TrentBoyett
Aspiring Mycologist



Registered: 11/29/12
Posts: 16,000
Loc: Kazakhstan
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Re: Wbr fractional sterilization? [Re: PussyFart]
#19106938 - 11/08/13 05:51 PM (10 years, 2 months ago) |
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Quote:
Notahacker420 said: Fractional sterilization fails 90% of the time.
If you cannot PC grains, steam them for about 8 hours.
That being said, not sure about whole rice...should be able to be just steamed.
BRF can just be steamed, but I dunno.
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RogerRabbit
Bans for Pleasure



Registered: 03/26/03
Posts: 42,214
Loc: Seattle
Last seen: 11 months, 4 days
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Re: Wbr fractional sterilization? [Re: jpack666]
#19107229 - 11/08/13 07:14 PM (10 years, 2 months ago) |
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Regardless of sterilization method, whole brown rice is a poor spawn choice. Too starchy. There are other, more suitable grains available. RR
-------------------- Download Let's Grow Mushrooms semper in excretia sumus solim profundum variat "I've never had a failed experiment. I've only discovered 10,000 methods which do not work." Thomas Edison
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Wbr fractional sterilization? [Re: PussyFart]
#19111716 - 11/09/13 07:15 PM (10 years, 2 months ago) |
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Quote:
RogerRabbit said: Regardless of sterilization method, whole brown rice is a poor spawn choice. Too starchy. There are other, more suitable grains available. RR
I agree, there is a lot of prep needed with this grain...
Now though I'm about to prepare some agar for plates with no PC... So I hope I find a way to succeed...
Quote:
Notahacker420 said: Fractional sterilization fails 90% of the time.
If you cannot PC grains, steam them for about 8 hours.
That being said, not sure about whole rice...should be able to be just steamed.
BRF can just be steamed, but I dunno.
Assuming I want to try without a PC....
Do you have experiences with many mediums when you say this? Of course there would be things like wbs millet rye in one category, then wbr or grass seed in another, than popcorn in another...
And also you would have agar mediums
Because in all honesty, I see successful fractional sterilization teks working fine on things like pop corn and agar, but I've never seen an example on a different medium...
And regarding the idea of steaming them for 8hrs... Does this, IYE, work best with a certain kind of grain/agar?
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Re: Wbr fractional sterilization? [Re: jpack666]
#19111762 - 11/09/13 07:27 PM (10 years, 2 months ago) |
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Quote:
jpack666 said: Because in all honesty, I see successful fractional sterilization teks working fine on things like pop corn and agar, but I've never seen an example on a different medium...
You can technically steam sterilize agar and it should be fine.....don't know why anyone would try fractional sterilization on that.
But I can link you to hundreds of threads where fractional sterilization either failed, or the OP never posted back, most likely because it failed.
I have never tried fractional sterilization, because I was serious about this hobby and bought a PC.
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THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Enigma1
Positive



Registered: 08/15/13
Posts: 977
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Re: Wbr fractional sterilization? [Re: jpack666]
#19111785 - 11/09/13 07:31 PM (10 years, 2 months ago) |
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Quote:
jpack666 said: Hi all 
I've seen teks for fractional sterilization for about every possible grain except wbr?
Anyone has every tried something like that? Brown rice grain without a PC?
Thanks
Jpack
Tried it to try it 3 out of 4 jars contamed.
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Wbr fractional sterilization? [Re: Enigma1]
#19111864 - 11/09/13 07:53 PM (10 years, 2 months ago) |
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Quote:
Enigma1 said:
Quote:
jpack666 said: Hi all 
I've seen teks for fractional sterilization for about every possible grain except wbr?
Anyone has every tried something like that? Brown rice grain without a PC?
Thanks
Jpack
Tried it to try it 3 out of 4 jars contamed.
Good stuff Enigma! Thanks for sharing your exprience...
If I may... Did you do it over 3 days? And how soon did you notice contams in your other 3 jars??
Thanks
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 17 days, 14 hours
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Re: Wbr fractional sterilization? [Re: jpack666]
#19111871 - 11/09/13 07:55 PM (10 years, 2 months ago) |
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It should not matter how long contams took to show up.
The fact that they showed up at all deems it a failure.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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Pastywhyte
Say hello to my little friend



Registered: 09/15/12
Posts: 37,810
Loc: Canada
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Re: Wbr fractional sterilization? [Re: PussyFart]
#19111977 - 11/09/13 08:31 PM (10 years, 2 months ago) |
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Close don't cut it in this hobby you never see anyone post that they almost got their grains sterile and check out these fruits.
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Wbr fractional sterilization? [Re: Pastywhyte]
#19112047 - 11/09/13 08:47 PM (10 years, 2 months ago) |
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Lol... I was focussing on the 25% success... But I get your point...
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Wbr fractional sterilization? [Re: jpack666]
#19112083 - 11/09/13 08:58 PM (10 years, 2 months ago) |
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Anyways right now I'm crossing my fingers to knock up hpoo with more myc so for now I may still have a chance at prints without a PC. I am -- for the time being -- very poor...
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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jpack666



Registered: 10/01/13
Posts: 484
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Re: Wbr fractional sterilization? [Re: Pastywhyte]
#19112110 - 11/09/13 09:03 PM (10 years, 2 months ago) |
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Quote:
Pastywhyte said:you never see anyone post that they almost got their grains sterile and check out these fruits.
I'm writing this down, just in case, you never know lol
-------------------- "There's a negative and a positive to everything." For more information, RogerRabbit's Website/Videos
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