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Offlineforrest
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19093148 - 11/05/13 11:05 PM (10 years, 3 months ago)

you might transfer them from the pc to the glovebox, and heat the air in thje glovebox with a hairdryer, only for a moment when you put them in.


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Invisibleliamtheloser
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: forrest]
    #19093331 - 11/05/13 11:48 PM (10 years, 3 months ago)

Try putting them in a foam cooler after the PC


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: liamtheloser]
    #19106287 - 11/08/13 03:33 PM (10 years, 3 months ago)

So, a few days later, I'm starting to see (and not see) some results from some of the agar work recently did.

The single drops on agar plates from the new liquid culture syringes aren't looking so hot thusfar.  I'm not sure if that's normal for only 3 days out, or not, though.

Unfortunately, the Pearl Oyster syringe looks to be contaminated, from what I can tell.  out of the three plates I put a drop of LC on, all three seem to be developing mold:








The King and enoki more or less look like this, which seems like nothing's happening.  I expected to see mycelium strands by now.



The only one that looks like it MIGHT be growing is ONE of the king oyster plates, which seems to have a raised section with some thin strands reaching up out of it.  I don't know if you can really see it from this picture, though.



The last experiment I had done was attempting to clone a grocery store yellow oyster to agar, which seems to have "fuzzed" up quite nicely but what I assume is mycelial growth doesn't seem to be running on the agar.




The agar this time around I added some blue food coloring to, about 4 drops for 600ml of agar.  I don't think this would cause a problem with growth on it, though, would it?


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OfflineJeff
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19106319 - 11/08/13 03:40 PM (10 years, 3 months ago)

The coloring doesn't hurt growth but it sure makes it hard to discern contams especially once you get it on grain.  You'll be seeing green everywhere.  I died some agar blue about a year ago just for novelty.  I swore I would never do it again.


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: Jeff]
    #19106334 - 11/08/13 03:44 PM (10 years, 3 months ago)

Yeah, the novelty has already worn off.  I don't even remember why I thoguht it would be a good idea in the first place.  I think I was thinking about making it potentially easier to see the mycelium to track sectors?  No clue...

Oh well, I have plenty of supplies and plenty of LC in those syringes, so I'll give it another round this weekend with some uncolored plates.


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OfflineJeff
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19106359 - 11/08/13 03:49 PM (10 years, 3 months ago)

Oh and by the way, I can see why an x-acto would be hard to deal with as you mentioned in a post above.  I use one like this..scalpel it is a bit more narrow than an x-acto and fits nicely in your hand.


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: Jeff]
    #19106426 - 11/08/13 04:02 PM (10 years, 3 months ago)

Ahh.  Yep, that would probably fit into the more narrow opening of these tubes.

The amber colored vials I bought as well seem to work just fine, thought, and I have a ton of them.  I just figure I need to devise a way to PC the slants at an angle, so they can cool gradually and that way I figure the condensation should be less of an issue.


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OfflineForresterM
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19108204 - 11/08/13 10:58 PM (10 years, 3 months ago)

That enoki looks like you put more than a drop on there, or there was condensation issues (I've been having plenty of that lately - many of my LC --> agar attempts have failed).
3 days isn't much though, give it more time and you'll be able to see better which are contaminated and which are good.  Some of mine that were too wet like that ended up ok, they just kinda take longer and then grow from the whole pool of liquid.  Or they get green mold.  You'll know for sure either way :lol:


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: Forrester]
    #19110084 - 11/09/13 11:50 AM (10 years, 3 months ago)

Yeah I know one or two of the plates got two drops as opposed to one.  That may have been one of them.    I'll do another round of them today and will inoculate a couple grain jars with each, this weekend, as well. 

The pearl syringe worries me, though, seeing 3 out of 3 plates with green on them...


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Invisibleliamtheloser
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19111322 - 11/09/13 05:45 PM (10 years, 3 months ago)

IME lc to agar takes quite a while to grow. I did pearl oyster from spore Depot onto 3 plates. All 3 were fine but they took 2 weeks before I saw any real growth. The parts covered in lc turned pale pretty quick,  but I didn't see mycelium for over a week.


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: liamtheloser]
    #19113491 - 11/10/13 07:09 AM (10 years, 3 months ago)

Well thats encouraging, then.  I won't count these out just yet then.  Well, except the peal oyster...  Thats clearly contaminated at this point. I'll do some new plates with that syringe.


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19113540 - 11/10/13 07:29 AM (10 years, 3 months ago)

Any thoughts on why the samples from the grocery store oyster clone attempts would fuzz up but not start running on the agar?  Or does it take a little while extra for the mycelium to "figure that out"?


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OfflineForresterM
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19113590 - 11/10/13 07:48 AM (10 years, 3 months ago)

Quote:

socraticd said:
Any thoughts on why the samples from the grocery store oyster clone attempts would fuzz up but not start running on the agar?  Or does it take a little while extra for the mycelium to "figure that out"?




So it's only been 5 days?  I'd give it more time.


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Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here.
-------------------

Have some medicinal mushrooms and want to get the most out of them?  Try this double extraction method.


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: Forrester]
    #19113806 - 11/10/13 08:58 AM (10 years, 3 months ago)

Yeah.  Well the grocery store yellow oyster clne is all fuzzed up and looks like mycelium reaching around.  Just hasn't taken to the agar yet.  The LC -> agar plates are still mostly barren but a couple look like they may be starting to grow.  I'm sure I'll know in a couple days


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Invisibleliamtheloser
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19114361 - 11/10/13 11:18 AM (10 years, 3 months ago)

What nutrient recipe are you using in your agar? I find if I made it too weak they take longer to spread initially. Too strong and they spread too fast. But even if I'm plating a wedge that someone sent me it usually takes a week or more for it to spread onto the new agar.


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: liamtheloser]
    #19133527 - 11/13/13 09:40 PM (10 years, 3 months ago)

Finally got a chance to prep some jars of rye for the first tim ever.  SO much nicer to work with than birdseed!

I inoculated 5 of 9 jars with the king oyster, pearl oyster, and enoki LCs that Ive been trying to get to take on agar (updates on those tomorrow) and the other four I put aside to attempt my first agar wedge inoculations.



I cut the plate in to four wedges and dropped one in each of the four jars.  Two of them I shook the hell out of and two I left alone after simply dropping the wedge on top.  Here's one post transfer.



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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19133568 - 11/13/13 09:50 PM (10 years, 3 months ago)

Oh, and liam, my agar recipe is 3% - 

600ml water
18g malt extract
10g agar


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Invisibleliamtheloser
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19134417 - 11/14/13 01:51 AM (10 years, 3 months ago)

Ahh mea, good choice. Nice and cheap

You honestly need like 1/16th of a plate or less as an inoculation. That is a huge wedge. I use small 60mm plates and get 8 wedges per plate.


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: liamtheloser]
    #19135100 - 11/14/13 08:17 AM (10 years, 3 months ago)

Yeah I know i could use a MUCH smaller wedge.  But I only had 4 jars prepped for this and I have probably 8 more fully colonized reishi plates after this one, so I figured I'd just use the whole plate.  :-)


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Offlinesocraticd
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Re: Socraticd's experiments - a.k.a, a newbie's grow log [Re: socraticd]
    #19135108 - 11/14/13 08:19 AM (10 years, 3 months ago)

Anyone have amy thoughts about the pearl oyster plates?  I inoculated 3 more plates and one of my grain jars with it.  If those all show contams like the first three above seemed to, would it be safe to say the syringe is contam'd and ask the vendor for a replacement?


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