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PhytoExtractum Shop: Maeng Da Thai Kratom Leaf Powder

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Invisibleblindingleaf
blue collar underworld
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Registered: 07/19/13
Posts: 22,008
Loc: sub-surface unseen
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2 agar question
    #19105599 - 11/08/13 01:05 PM (10 years, 2 months ago)

hey guys.
I have been working with agar recently, trying to isolate.  With these first 2 pics, There are three transfer sites (I don't have segmented petris yet, they are coming today).  The first two pics are the same, and I was wondering if the transfer on the 9 o clock position and the transfer on the 6 o clock position on the 2nd pic (same transfer, but dish in different position) was an isolate?  Its hard to tell for me, because all the strands look rhizo, but they are not as connected near the base as several pics I have seen.  As you can tell, I transferred from the plate in a different spot.




Now these 2 pics are from fresh tissue transfer.  From what I can tell, they look like Linear (longitudinally radial) mycelium, a la stamets TMC.  Does that look correct?  If so, all the TMC says about this is "Linear mycelium can also produce abundant primordial but this usually occurs soon after it forms rhizomorphs." (TMC, pp.33)  Soo...is this plate worth working with, as a beginner agar user, or something I can mess around with as I get more comfortable with agar?  They are the same plate, 2nd pic is from underside.





Any help would be awesome, you guys got me this far...


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A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

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Invisibleblindingleaf
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Registered: 07/19/13
Posts: 22,008
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Re: 2 agar question [Re: blindingleaf]
    #19110739 - 11/09/13 02:53 PM (10 years, 2 months ago)

bumping after 24 hours...really wanna get an opinion on this... also any info on linear mycelium


--------------------
A few thoughts on cultivation
MICROBIAL HUSBANDRY!!!!

The whole is greater than the sum of its parts


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