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OfflineThe Grump
Stranger
Registered: 11/08/13
Posts: 27
Last seen: 3 years, 7 months
Into the medium instead of sides: how bad did I screw up?
    #19103915 - 11/08/13 03:01 AM (10 years, 2 months ago)

It's my first time, so to make it easy I bought pre-made jars from a vendor (very prompt delivery!)  I ended up using their oven inoculation method.  Went with 18 jars for first time in case some fail (and trying 3 different strains, B+, Creeper, and Koh Samui Super Strain, 6 jars each).  Lysol all over the place, fresh clean clothing, filter mask, rubber gloves, etc.  There were two things I did that did not follow exact instructions:
1) I did not torch the tip with a lighter on first use, I've since read to do this from the start, but since the needle came direct out of its sealed sterile package, I figured it did not need to be lighter burned/alcohol swabbed until after the first jar inoculation (I have a feeling I'm fine here, I mean, it was a sterile medical needle of course, don't see why I would need to flame it the first time.

2) The main issue:  I did not realize until more reading now the importance of getting the spore solution down the sides of the glass.  When I injected, I did not always have the needle angled to the side of the glass, sometimes it was straight down into the medium (full tip insertion, to get past the top layer, of course), other times at an angle where maybe some reached the sides, some maybe not.  Also being a first timer, it took me a bit to get used to how much pressure to put on the syringe stopper, sometimes it was "oops".  I had three syringes and 18 jars, so more than enough solution, and I used it all.  Three of the jars are very lightly inoculated, so I'm not expecting a lot from them, some of the other jars have more than enough. 

So my main issue is 2, my goof in not getting it down the sides of the jar so much.  What might I expect?  Less yield, longer incubation time, or did I screw up to an extent where I should immediately order more spores and inoculate again?  Frustrated with myself, a major "doh" moment.

I'm still going to get SOMETHING, right?  : (




Edited by stonesun (11/08/13 09:18 AM)


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InvisibleBass808
Dr. Womp

Registered: 10/19/13
Posts: 32
Loc: Underground
Re: Into the medium instead of sides: how bad did I screw up? [Re: The Grump]
    #19103926 - 11/08/13 03:13 AM (10 years, 2 months ago)

Yes you should get something, shooting spores down the sides only allows you a better look for first signs of growth. You'll be fine. I do the same thing as far as new needles. If they haven't been opened they are sterile and you don't need too flame sterilize but it couldn't hurt. I wipe new needles with iso and go too work in a SAB.


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OfflineOgreLokon
Pretty Fun Guy
Male

Registered: 06/30/13
Posts: 512
Last seen: 6 years, 5 months
Re: Into the medium instead of sides: how bad did I screw up? [Re: Bass808]
    #19103936 - 11/08/13 03:21 AM (10 years, 2 months ago)

:whathesaid:


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OfflineThe Grump
Stranger
Registered: 11/08/13
Posts: 27
Last seen: 3 years, 7 months
Re: Into the medium instead of sides: how bad did I screw up? [Re: Bass808]
    #19103939 - 11/08/13 03:23 AM (10 years, 2 months ago)

Thanks!  I figured something still has to happen.  I might try building a SAB box next time, but thought I would try the oven method work (supposedly has a high success rate), sprayed lots of lysol first, in air and on all nearby surfaces and a bunch in the oven, etc,  so hopefully it was sterile enough!


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InvisibleSkinty
TOP SECRET


Registered: 07/04/13
Posts: 1,150
Re: Into the medium instead of sides: how bad did I screw up? [Re: Bass808]
    #19104070 - 11/08/13 05:50 AM (10 years, 2 months ago)

Quote:

Bass808 said:
Yes you should get something, shooting spores down the sides only allows you a better look for first signs of growth.




Also allows generally for quicker visual ID of contams :thumbup:


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OfflineGiggle_Grower
A lil less noob each day
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Registered: 04/07/10
Posts: 1,598
Loc: Shroomery
Last seen: 1 year, 5 months
Re: Into the medium instead of sides: how bad did I screw up? [Re: Skinty]
    #19104081 - 11/08/13 06:04 AM (10 years, 2 months ago)

Quote:

sprayed lots of lysol first, in air and on all nearby surfaces and a bunch in the oven, etc, so hopefully it was sterile enough!




Dude you cook in that oven. Do you really want lysol in there? Build a SAB, its soooo worth it. Otherwise it sounds like your on the right path, aside from.a grow kit. They can work if used right, but nothing beats doing it your self! And its cheaper. As said, your spores are fine, they will still grow, it will just be a little longer before you see their growth. Watch that KSSS I hear its a crazy mofo!


--------------------
I'm  always interested in trades.
The Awesome Purple Mystics
Noobs! The best tool here is up top to the right. Don't  forget about it. Just type your question in!:cool:
RR is my favorite source of knowledge. Check out his videos!

If I forgot to leave you a rating, please remind me.


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InvisiblePastywhyteMDiscord
Say hello to my little friend
Male User Gallery


Registered: 09/15/12
Posts: 37,810
Loc: Canada Flag
Trusted Cultivator
Re: Into the medium instead of sides: how bad did I screw up? [Re: Bass808]
    #19104138 - 11/08/13 06:43 AM (10 years, 2 months ago)

Quote:

Bass808 said:
I wipe new needles with iso and go too work in a SAB.




You shouldn't do that. Iso alcohol is not sterile.

Quote:

RogerRabbit said:
Wiping a loop with alcohol after flame sterilizing is like wiping shit on your face after taking a shower.
RR



http://www.shroomery.org/forums/showflat.php/Number/15041493#15041493

Quote:

RogerRabbit said:
I've always been curious why anyone would flame a needle, and then wipe it with alcohol, as if anything could be cleaner than red hot.  It would also be nice if someone could explain how wiping with alcohol can clean the inside of the needle, which to a contaminant spore is the size of the English Channel.

I suggest to flame sterilize until red hot, and then use.  Save your alcohol for the jar lid/tape that you'll be injecting through, and your gloves, etc.
RR




http://www.shroomery.org/forums/showflat.php/Number/10649875#10649875


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InvisiblebodhisattaMDiscordReddit
Smurf real estate agent
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Folding@home Statistics
Registered: 04/30/13
Posts: 61,889
Loc: Milky way
Trusted Cultivator
Re: Into the medium instead of sides: how bad did I screw up? [Re: Pastywhyte]
    #19104356 - 11/08/13 08:34 AM (10 years, 2 months ago)

:whathesaid:
and edit your post remove the sponsor's name. We don't use sponsors products to cultivate actives. Are you trying to screw some good people over?

You really should read the rules before you post man...


Edited by Trusted cuItivator (11/08/13 08:35 AM)


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