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Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
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itinkitook2much
Psychonaut



Registered: 09/04/13
Posts: 180
Loc: CANADA
Last seen: 2 years, 4 months
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TROUBLE WITH PF TEK
#19099933 - 11/07/13 11:16 AM (10 years, 3 months ago) |
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Didn't see any colonization after 5 days, I THINK(hope) that the B.R.F cakes were properly made... 240 ml water 480 ml ground up long grain brown rice 240 ml vermiculite
Got a couple questions on what I might have done wrong.. -Could I use my crock pot for 90 mins for the sterilization? -Does the water, vermiculite or brf go in first for mix (does it even matter) -Should I have gotten organic rice or just plain brown rice in the grinder is fine? -Reptile heat mat too hot for the jar ? -After inoculation do I seal all 4 holes with tape+foil again? -Do I seal all holes during sterilization? -I didn't use a 1/2 pint jar, I used the 500 ml ones.. do they need much more spores? AND MOST IMPORTANT QUESTION : How long after sterilization do i Inoculate spores ?!?!
Thanks guys.. I really hope I see some Mycelium running soon
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screamingdeal
Stranger
Registered: 10/15/13
Posts: 61
Last seen: 3 years, 2 months
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Follow this step by step, thats what ive been doing, so far no problems
http://www.shroomery.org/8410/Simple-Cubensis-Growing-Technique
-------------------- There are two kinds of people in this world, ones who can finish lists and.... No cutting in the Kool-Aid line. -Jim Jones
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urthtown
meat popsicle


Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 2 years, 4 months
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Quote:
itinkitook2much said: Didn't see any colonization after 5 days, I THINK(hope) that the B.R.F cakes were properly made... 240 ml water 480 ml ground up long grain brown rice 240 ml vermiculite
Got a couple questions on what I might have done wrong.. -Could I use my crock pot for 90 mins for the sterilization? -Does the water, vermiculite or brf go in first for mix (does it even matter) -Should I have gotten organic rice or just plain brown rice in the grinder is fine? -Reptile heat mat too hot for the jar ? -After inoculation do I seal all 4 holes with tape+foil again? -Do I seal all holes during sterilization? -I didn't use a 1/2 pint jar, I used the 500 ml ones.. do they need much more spores? AND MOST IMPORTANT QUESTION : How long after sterilization do i Inoculate spores ?!?!
Thanks guys.. I really hope I see some Mycelium running soon
First off, you didn't follow the tek properly - watch RR's video: http://www.mushroomvideos.com/BRF-Pf-Tek
A crock pot may not get hot enough - you want it to boil with the jars elevated so they are in a 90 minute long steam bath. Use a pot with a tight fitting lid.
It can take up to 2 weeks to see myc growth in jars. Be patient. If you use the search bar you'll find plenty of people with successful grows that took longer than 5 days to show initial growth.
You want 1:2:1, BRF:VERM:H20 (EDITED: MY BAD!!) looks like you swapped the verm and BRF amounts.
As per the video and other teks, mix water and verm - then add flour.
Organic prob not going to make any diff except to your conscience.
You shouldn't be heating them... you should keep them at close to 80df ambient temp but if you search the forum, people have colonized well at lower temps ~70df without issue.
FOLLOW THE TEK - don't seal holes, these are required for gas exchange and your layer of dry vermiculite (IF you followed the tek :/) is your filter.
You cover the holes with foil during sterilization to prevent water from getting into your dry layer of verm, then remove after sterilization.
Your jars are twice as big as they should be... logic says you could use more spores. Next time follow the tek.
AS PER THE TEK - wait 8 hours, overnight, or until the jars have cooled completely to room temp before inoculating.
AND THE MOST IMPORTANT ANSWER: you could've answered every one of these questions by watching RR's video
Take it from me - I'm a noob and didn't find the SGFC tek until after having built it incorrectly. You don't know more than mycology than these people, just READ and then FOLLOW the tek!!!
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
Edited by urthtown (11/07/13 11:51 AM)
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OgreLokon
Pretty Fun Guy


Registered: 06/30/13
Posts: 512
Last seen: 6 years, 6 months
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Re: TROUBLE WITH PF TEK [Re: urthtown]
#19100019 - 11/07/13 11:33 AM (10 years, 3 months ago) |
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Quote:
urthtown said:
Quote:
itinkitook2much said: Didn't see any colonization after 5 days, I THINK(hope) that the B.R.F cakes were properly made... 240 ml water 480 ml ground up long grain brown rice 240 ml vermiculite
Got a couple questions on what I might have done wrong.. -Could I use my crock pot for 90 mins for the sterilization? -Does the water, vermiculite or brf go in first for mix (does it even matter) -Should I have gotten organic rice or just plain brown rice in the grinder is fine? -Reptile heat mat too hot for the jar ? -After inoculation do I seal all 4 holes with tape foil again? -Do I seal all holes during sterilization? -I didn't use a 1/2 pint jar, I used the 500 ml ones.. do they need much more spores? AND MOST IMPORTANT QUESTION : How long after sterilization do i Inoculate spores ?!?!
Thanks guys.. I really hope I see some Mycelium running soon
First off, you didn't follow the tek properly - watch RR's video: http://www.mushroomvideos.com/BRF-Pf-Tek
A crock pot may not get hot enough - you want it to boil with the jars elevated so they are in a 90 minute long steam bath. Use a pot with a tight fitting lid.
It can take up to 2 weeks to see myc growth in jars. Be patient. If you use the search bar you'll find plenty of people with successful grows that took longer than 5 days to show initial growth.
You want 1:2:1, BRF:H20:vermiculite looks like you swapped the verm and BRF amounts.
As per the video and other teks, mix water and verm - then add flour.
Organic prob not going to make any diff except to your conscience.
You shouldn't be heating them... you should keep them at close to 80df ambient temp but if you search the forum, people have colonized well at lower temps ~70df without issue.
FOLLOW THE TEK - don't seal holes, these are required for gas exchange and your layer of dry vermiculite (IF you followed the tek :/) is your filter.
You cover the holes with foil during sterilization to prevent water from getting into your dry layer of verm, then remove after sterilization.
Your jars are twice as big as they should be... logic says you could use more spores. Next time follow the tek.
AS PER THE TEK - wait 8 hours, overnight, or until the jars have cooled completely to room temp before inoculating.
AND THE MOST IMPORTANT ANSWER: you could've answered every one of these questions by watching RR's video
Take it from me - I'm a noob and didn't find the SGFC tek until after having built it incorrectly. You don't know more than mycology than these people, just READ and then FOLLOW the tek!!!
Quote:
urthtown said:
Quote:
itinkitook2much said: Didn't see any colonization after 5 days, I THINK(hope) that the B.R.F cakes were properly made... 240 ml water 480 ml ground up long grain brown rice 240 ml vermiculite
Got a couple questions on what I might have done wrong.. -Could I use my crock pot for 90 mins for the sterilization? -Does the water, vermiculite or brf go in first for mix (does it even matter) -Should I have gotten organic rice or just plain brown rice in the grinder is fine? -Reptile heat mat too hot for the jar ? -After inoculation do I seal all 4 holes with tape+foil again? -Do I seal all holes during sterilization? -I didn't use a 1/2 pint jar, I used the 500 ml ones.. do they need much more spores? AND MOST IMPORTANT QUESTION : How long after sterilization do i Inoculate spores ?!?!
Thanks guys.. I really hope I see some Mycelium running soon
First off, you didn't follow the tek properly - watch RR's video: http://www.mushroomvideos.com/BRF-Pf-Tek
A crock pot may not get hot enough - you want it to boil with the jars elevated so they are in a 90 minute long steam bath. Use a pot with a tight fitting lid.
It can take up to 2 weeks to see myc growth in jars. Be patient. If you use the search bar you'll find plenty of people with successful grows that took longer than 5 days to show initial growth.
You want 1:2:1, BRF:H20:vermiculite looks like you swapped the verm and BRF amounts.
As per the video and other teks, mix water and verm - then add flour.
Organic prob not going to make any diff except to your conscience.
You shouldn't be heating them... you should keep them at close to 80df ambient temp but if you search the forum, people have colonized well at lower temps ~70df without issue.
FOLLOW THE TEK - don't seal holes, these are required for gas exchange and your layer of dry vermiculite (IF you followed the tek :/) is your filter.
You cover the holes with foil during sterilization to prevent water from getting into your dry layer of verm, then remove after sterilization.
Your jars are twice as big as they should be... logic says you could use more spores. Next time follow the tek.
AS PER THE TEK - wait 8 hours, overnight, or until the jars have cooled completely to room temp before inoculating.
AND THE MOST IMPORTANT ANSWER: you could've answered every one of these questions by watching RR's video
Take it from me - I'm a noob and didn't find the SGFC tek until after having built it incorrectly. You don't know more than mycology than these people, just READ and then FOLLOW the tek!!!
Incorrect- 2:1:1 verm:h2o:BRF
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Peachette
Wayward Princess



Registered: 11/22/09
Posts: 681
Loc: Secret Dungeon Level
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Re: TROUBLE WITH PF TEK [Re: OgreLokon]
#19100057 - 11/07/13 11:39 AM (10 years, 3 months ago) |
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I'd worry more a bout the heat mat and the crock pot first.
Getting the PFTek down is important, read the link above, but if you cannot get rid of the crock pot or the heat mat you're just going to have more issues down the road. Use smaller jars for your first go-around.
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PussyFart
Retired Cultivation Extrodinaire



Registered: 04/08/12
Posts: 22,502
Loc: Orbiting Earth
Last seen: 1 month, 10 days
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Re: TROUBLE WITH PF TEK [Re: Peachette]
#19100066 - 11/07/13 11:41 AM (10 years, 3 months ago) |
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Jars/bags/tubs/trays should colonize @ room temperature getting ambient/indirect light.
Main pinning triggers are full colonization, FAE and Evaporation off of the substrate.
Light is a secondary pinning trigger. For tropical species temperature is not a pinning factor.
P. Cubensis are a tropical species. You could colonize at 70F and fruit at 80F with great results.
Light has been proven beneficial during all stages of mycellium growth. Mushrooms like mammals have a circadian rhythm.
You want ambient/indirect light(on a 12/12 schedule preferably) for colonization and consolidation.
You want direct/intense 6500K light on a 12/12 schedule for fruiting.
Optimal temps are mid 70s throughout the whole grow, but anywhere from 65F-80F is acceptable.
Incubation is outdated/uneeded unless temps in the range stated above cannot be kept.
The inside of the jar is always a few degrees warmer than the outside because the mycellium produces heat..mycellium tends to stall at temps above 86F , and contams thrive.
Fruiting at cooler temps tends to produce denser, meatier fruits, while fruiting at higher temps will often produce hollow, less dense stems.
--------------------
THIS HOBBY IS NOT FOR THE IMPATIENT! PLEASE BE PATIENT, DON'T BE A PATIENT! A Tale of 10 Isolates, GT Cluster Clone Monotubs, RR's Let's Grow Mushrooms DVD, SGFC(Shotgun Fruiting Chamber), Monotub Tek, Damion5050's Coir Tek, TL's Tek List, Frank's Tek List, EvilMushroom666's Pasteurization Tek, How It Should & Shouldn't Look - NEW CULTIVATORS GUIDE *** *** AFGHAN KUSH GROW LOG *** ***
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urthtown
meat popsicle


Registered: 10/26/13
Posts: 1,039
Loc: Eastern Canadia
Last seen: 2 years, 4 months
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Re: TROUBLE WITH PF TEK [Re: OgreLokon]
#19100108 - 11/07/13 11:53 AM (10 years, 3 months ago) |
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Quote:
OgreLokon said:
Incorrect- 2:1:1 verm:h2o:BRF
my bad - that's what I was trying to write. Edited original reply to avoid confusing people even more.
Long story short, watch the RR video and follow what he says.
-------------------- Cluster Headache sufferer? Cluster Busting Veil Tear GIF Flower Pot Grow GIF Mini Mono Tub GIFS "All mushrooms are edible, but some only once." -- Croatian Proverb
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itinkitook2much
Psychonaut



Registered: 09/04/13
Posts: 180
Loc: CANADA
Last seen: 2 years, 4 months
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Re: TROUBLE WITH PF TEK [Re: urthtown]
#19100151 - 11/07/13 12:04 PM (10 years, 3 months ago) |
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Thanks a FUCK lot guys man I had already ordered the 1/2 pint jars online while I was doing first attempt.. I was pretty fucking stoned when I was watching the boring as fuck RR videos, I must've written down the wrong ingredients.. I'l post up results of my second try!!! THANKS AGAIN!!! 
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Peachette
Wayward Princess



Registered: 11/22/09
Posts: 681
Loc: Secret Dungeon Level
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It's all about trial and error :]
Save those jars for future use!
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itinkitook2much
Psychonaut



Registered: 09/04/13
Posts: 180
Loc: CANADA
Last seen: 2 years, 4 months
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Re: TROUBLE WITH PF TEK [Re: Peachette]
#19100621 - 11/07/13 01:37 PM (10 years, 3 months ago) |
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Random shit but I did IN FACT write down the proper ingredient amounts, where I fucked up was re-writing it here on the post lol.. 240mlBRF,240mlH20,480mlVERM, so all I have to do is wait for some mycelium I guess.. I Inoculated a couple of minutes after it was sterilized inside the crock pot. Like in the RR using jar lids, water meets bottom of the jars and with vapors for 90 minutes, these steps are all well done,, Maybe I could have waited till the jar completely cooled down before inoculation..
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bodhisatta 
Smurf real estate agent



Registered: 04/30/13
Posts: 61,890
Loc: Milky way
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Quote:
itinkitook2much said: Maybe I could have waited till the jar completely cooled down before inoculation..
Yes... You should have. Patience is a protip for success in this hobby.
Using that crockpot did the water come to a hard boil?
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1down5up
Social Ninja


Registered: 11/04/13
Posts: 1,743
Last seen: 1 year, 3 months
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Re: TROUBLE WITH PF TEK [Re: bodhisatta]
#19100976 - 11/07/13 02:39 PM (10 years, 3 months ago) |
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I'm a complete noob myself, but i think you should follow the advice on the video. Even crack heads on the street can find a pot, and ask a friend for a lid. scrap the crock pot.
On a side note, if you find the video boring as fuck, it's no wonder you're doing it wrong. Seriuosly man...watch it, and put the joint down this time...lol
-------------------- ~~Everything is relative~~ A Simplified Overview of Mushroom Cultivation Strategies - RR says - EvilMushroom666's Take on BRF Cakes - Frank's list of goodies - Cronicr's Goodies No one is placed higher than another no matter race or creed or gender, we must teach forgiveness and compassion for all life. J.L.
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cronicr



Registered: 08/07/11
Posts: 61,436
Loc: Van Isle
Last seen: 2 years, 1 month
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Re: TROUBLE WITH PF TEK [Re: 1down5up]
#19101006 - 11/07/13 02:44 PM (10 years, 3 months ago) |
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Quote:
1down5up said: I'm a complete noob myself, but i think you should follow the advice on the video. Even crack heads on the street can find a pot, and ask a friend for a lid. scrap the crock pot.
On a side note, if you find the video boring as fuck, it's no wonder you're doing it wrong. Seriuosly man...watch it, and put the joint down this time...lol 
horrrible advise...DON'T EVER PUT YOUR JOINT DOWN TILL YOUR FINGERS BURN
--------------------
  It doesn't matter what i think of you...all that matters is clean spawn I'm tired do me a favor
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Fungi Tunic
Strange
Registered: 10/08/13
Posts: 101
Loc: Seattle
Last seen: 3 years, 9 months
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Re: TROUBLE WITH PF TEK [Re: cronicr]
#19101762 - 11/07/13 05:08 PM (10 years, 3 months ago) |
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As an aside, I recently found out that my local Wholefoods bulk BRF stinks a good bit after being steam sterilized. Red Mill does not, unknown effect on the grow itself as I just knocked em up.
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itinkitook2much
Psychonaut



Registered: 09/04/13
Posts: 180
Loc: CANADA
Last seen: 2 years, 4 months
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Re: TROUBLE WITH PF TEK [Re: cronicr]
#19101808 - 11/07/13 05:16 PM (10 years, 3 months ago) |
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Quote:
bodhisatta said:
Quote:
itinkitook2much said: Maybe I could have waited till the jar completely cooled down before inoculation..
Yes... You should have. Patience is a protip for success in this hobby.
Using that crockpot did the water come to a hard boil?
well it was boiling but I don't know about HARD boil lol, many tiny bubbles and if i took the lid off there was a lot of steam that flew out.. so i left it closed for the remaining time after having lost much steam... Good enough? Im waiting for the 1/2 pint jars I can't wait for my 2nd shot and doing it RIGHT this time !!  Quote:
cronicr said:
Quote:
1down5up said: I'm a complete noob myself, but i think you should follow the advice on the video. Even crack heads on the street can find a pot, and ask a friend for a lid. scrap the crock pot.
On a side note, if you find the video boring as fuck, it's no wonder you're doing it wrong. Seriuosly man...watch it, and put the joint down this time...lol 
horrrible advise...DON'T EVER PUT YOUR JOINT DOWN TILL YOUR FINGERS BURN
never
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Rubestoad
Stranger


Registered: 09/13/13
Posts: 231
Loc: your mamas porch. sunrise
Last seen: 8 years, 3 months
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Quote:
itinkitook2much said: Random shit but I did IN FACT write down the proper ingredient amounts, where I fucked up was re-writing it here on the post lol.. 240mlBRF,240mlH20,480mlVERM, so all I have to do is wait for some mycelium I guess.. I Inoculated a couple of minutes after it was sterilized inside the crock pot. Like in the RR using jar lids, water meets bottom of the jars and with vapors for 90 minutes, these steps are all well done,, Maybe I could have waited till the jar completely cooled down before inoculation..
Do not put down that joint! But if you inoculated per that post "a couple minutes after"you turned the heat of the pseudo sterilize then it was to hot for the spores to live. You will get no growth
-------------------- Go placidly amid the noise and haste, and remember what peace there may be in silence.
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elasticaltiger
Like Tigers in Coitus




Registered: 06/24/13
Posts: 8,403
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Quote:
itinkitook2much said: well it was boiling but I don't know about HARD boil lol, many tiny bubbles and if i took the lid off there was a lot of steam that flew out.. so i left it closed for the remaining time after having lost much steam... Good enough?
No.
The water has to first hit a hard rolling boil. Then turn the heat down until you're left with a constant audible bubbling (big bubbles.)
I've never worked with any crockpot that can maintain a full boil. A 'simmer' is not the same. Sorry man.
-------------------- First time growing cakes? DON'T make a Shotgun Fruiting Chamber The Shmuvbox. - The Old TC's Like it Afraid to Start Growing From Your Own Prints? Drop it Like a Tiger! No Pouring. No Syringes. No Cutting. No flaming. No Contamination. No Bullshit. "The best thing to do while your waiting is to start more stuff. I usually got so much happening that I have tossed projects simply because I didn't have time for them. -Pastywhite QFT Pastywhite's Easy Agar Tek (PastyPlates) Tiger Drop Video Demos By munchauzen Van Gogh would’ve sold more than one painting if he’d put tigers in them.―Bill Watterson EZEKIEL 23:20
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