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InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
old MS print on agar
    #19088853 - 11/05/13 07:10 AM (10 years, 2 months ago)

just wondering how long to wait for signs of growth from MS print innoc on agar? Print is over five years old... will warmer temps help? it's maybe around 65*.


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OfflinePussyFart
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Registered: 04/08/12
Posts: 22,502
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Re: old MS print on agar [Re: NOS4A2]
    #19088860 - 11/05/13 07:14 AM (10 years, 2 months ago)

Warmer than 65 might help a little bit, but it could take 2+ weeks to see growth in some cases.


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OfflineeLeSDenes
Mycelium Expander
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Registered: 05/25/11
Posts: 955
Last seen: 1 year, 11 months
Re: old MS print on agar [Re: PussyFart]
    #19088891 - 11/05/13 07:29 AM (10 years, 2 months ago)

Sometimes it helps if you put a drop of water over the spores on agar because the spores dehydrate over time. You can try to make the agar on the soupy side as well. Also you can try to put the spores in the agar not on it. Germination will take very long but this is all you can do.


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OfflineRubestoad
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Registered: 09/13/13
Posts: 231
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Re: old MS print on agar [Re: eLeSDenes]
    #19089000 - 11/05/13 08:22 AM (10 years, 2 months ago)

Another option would be to make a syringe from some of the print and let that sit for a week to hydrate the spores mabey


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Go placidly amid the noise and haste, and remember what peace there may be in silence.


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InvisibleNOS4A2
This is the way


Registered: 07/22/04
Posts: 572
Loc: -tite Flag
Re: old MS print on agar [Re: Rubestoad]
    #19089455 - 11/05/13 10:45 AM (10 years, 2 months ago)

OK, patience it is then
A syringe was made from a different print stored in a different place and inoc'ed PF jars with no effect. Took 3 months to contam! lol

This print was sealed in a specimen jar like the ones you have your pee put in during a random drug test so there is hope for better result.  heard seven years is the rule of thumb.

what's the oldest print anyone's revived here?


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