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Offlinenasser
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agaricus bisporus spores 2nd trasfere w\pics any advise
    #19088666 - 11/05/13 04:46 AM (10 years, 2 months ago)

hey
i tried several times to clone white button mushroom and as rr said i started with a spore print rather than a tissue from store bought mushroom so this is the result and am a little confused is it a white button mycelium or not the pictures shows a flufy growth at the edge of the colony and its my first time so i want to make sure its an agaricus bisporus mycelium
note: sorry for my bad English am not an English speaker but i try.


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InvisibleZen Peddler
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Re: agaricus bisporus spores 2nd trasfere w\pics any advise [Re: nasser]
    #19108890 - 11/09/13 02:22 AM (10 years, 2 months ago)

It certainly looks like mycelium although Id say you'd have to think about cleaning it up via quick transfers or h202 - quick transfers are healthier and quicker but some say lots of transfers just get the strain used to growing on agar, Im personally a fan of h202 to knock out any spores present and then its clean.

You can clone an old or dirty specimen also by dipping it in h202. Rip a mushroom in half in a glove box, flame your scalpel then take a section, dip it in h202 and then transfer to h202 agar if its really bad - this method has never failed me in 14 years...


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Offlinenasser
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Re: agaricus bisporus spores 2nd trasfere w\pics any advise [Re: Zen Peddler]
    #19109137 - 11/09/13 05:55 AM (10 years, 2 months ago)

so its a normal growth?
thank you i will try the h2o2


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InvisibleZen Peddler
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Re: agaricus bisporus spores 2nd trasfere w\pics any advise [Re: nasser]
    #19124999 - 11/12/13 05:26 AM (10 years, 2 months ago)

No idea what that white shit down the bottom is but it looksand is growing like mycelium. Agaricus I've seen on agar are usually a thin kind of mycelium but yours looks ok. I'd try and sector it or h202 it to clean it up. You may want to isolate different sectors and try out a few.


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