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Offlinedr.alkaline
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Registered: 12/15/12
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Is it better to make syringes from my prints or go to agar straight from print?
    #19087818 - 11/04/13 10:43 PM (10 years, 2 months ago)

I looked around on the search engine from this, but couldn't find a good answer.

I have been printing all of my decent size caps on aluminum, drying the print, and then swabbing a bit on agar with an innoc loop before mating the print to another piece of alum foil for long term storage. How many times can I transfer to agar from an individual print? I know prints are never 100% clean, but Is it likely going to be contaminated to an unusable point after doing this a few times. Is it better to make a few syringes from one print and inoculate the agar with the syringe when I need to?


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OfflineStromriderM
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Registered: 06/02/13
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Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: dr.alkaline]
    #19087934 - 11/04/13 11:15 PM (10 years, 2 months ago)

Straight to agar from print. Even if you happen to get a contam you can transfer away from it


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Offlinedr.alkaline
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Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: Stromrider]
    #19087946 - 11/04/13 11:17 PM (10 years, 2 months ago)

Can I just keep doing it from the same print, storing the print in back in the foil and bag between inoculations?


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: dr.alkaline]
    #19087962 - 11/04/13 11:21 PM (10 years, 2 months ago)

Definitely straight to agar. And yes, you can use the same print for years.


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InvisiblePestile

Registered: 05/02/13
Posts: 875
Loc: Northern Europe
Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: dr.alkaline]
    #19087971 - 11/04/13 11:23 PM (10 years, 2 months ago)

Definitely agar. And yes, you can use the same print for years.


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OfflineStromriderM
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Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: Pestile]
    #19088128 - 11/05/13 12:03 AM (10 years, 2 months ago)

Quote:

Pestilence said:
Definitely agar. And yes, you can use the same print for years.




:whathesaid:


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Offlinesilverstem
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Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: Pestile]
    #19088136 - 11/05/13 12:06 AM (10 years, 2 months ago)

why dont u just take a bit of ur mushroom and use it as an isolate instead of making a print then adding it to agar... pick a mushroom u really like. grab some flesh from the inside of the stem and put it on ur petri... that way u could grow with partially the same genetics as the isolate u picked... better then trying to hit the lotto with ms.


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Offlinedr.alkaline
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Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: silverstem]
    #19088944 - 11/05/13 07:56 AM (10 years, 2 months ago)

I like prints for safe keeping, and I am just getting started so I have not had a chance to try that, but I am working in that direction.


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InvisibleSpitballJedi
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Registered: 10/13/12
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Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: silverstem]
    #19089236 - 11/05/13 09:43 AM (10 years, 2 months ago)

Quote:

silverstem said:
why dont u just take a bit of ur mushroom and use it as an isolate instead of making a print then adding it to agar... pick a mushroom u really like. grab some flesh from the inside of the stem and put it on ur petri... that way u could grow with partially the same genetics as the isolate u picked... better then trying to hit the lotto with ms.




Your point is well taken, but it is not an isolate. What you describe is just cloning, but it will have many genetics.

Taking prints is common practice and has many benefits.


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OfflineDeadPhan
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Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: SpitballJedi]
    #19089268 - 11/05/13 09:51 AM (10 years, 2 months ago)

Quote:

SpitballJedi said:
Quote:

silverstem said:
why dont u just take a bit of ur mushroom and use it as an isolate instead of making a print then adding it to agar... pick a mushroom u really like. grab some flesh from the inside of the stem and put it on ur petri... that way u could grow with partially the same genetics as the isolate u picked... better then trying to hit the lotto with ms.




Your point is well taken, but it is not an isolate. What you describe is just cloning, but it will have many genetics.

Taking prints is common practice and has many benefits.




on that note, if i have a tub, that i made from an isolate on agar that i transferred to grain, then g2gd with, and the tub, performs decent, but maybe not out of this world amazing with even pinset and yield, however, i have a very awesome fruit from it, from, fast growing, to size, shape, etc, and i want to clone that fruit, am i to expect the same in yield and pinset from cloning that fruit, or is it just as much a crapshoot as the isolate itself was?

and with that, in that case, if not,  how does one ever know they have an isolate, or a clone, that will perform with a great yield and pinset as the one before it? 

is that only when you take a stab at it with an isolate, find out if performs well, and hopefully still have some plates of that isolate?


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InvisibleSpitballJedi
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Re: Is it better to make syringes from my prints or go to agar straight from print? [Re: DeadPhan]
    #19089355 - 11/05/13 10:17 AM (10 years, 2 months ago)

I'm by no means a master at isolating. But, sometimes, the untrained/inexperienced  eye may see an isolate on agar when in fact it is not.

Even when you have an isolate, what you see are the dominate genetics. The original MS genetics are still present, but recessive. This is why you can take a spore print from an isolate and be back to square one MS. In other words, a spore print from an isolate is still MS.

I'm no geneticist either, but that's is my simple understanding.

So, if what I'm saying is correct, it would stand to reason that your fruits from an isolate will not be all exact. The recessive genes still may show up.

Isolates, from my understanding, are the best way to obtain even pin-sets, flushes, and fruits. But, it is not considered to be a 100% guarantee.

When fruiting isolates, I would still take the clone from the fruit I like the best. If isolation was attempted on agar, then an individual fruit should still have a reduced number of genetics.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


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