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Jalidone
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Registered: 11/04/13
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First Mycological Experiment! NeEd SomE Help
#19087559 - 11/04/13 09:41 PM (10 years, 2 months ago) |
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Greetings fellow Mycologists, this is my first time experimenting with mushroom growing. I have quite a bit of experience growing cannabis, but I am extremely interested in being able to produce my own Psilocybin Cubensis mushrooms. So after watching many video and readying many guides I acquired the necessary materials to start a BRF Substrate grow. Materials acquired were, 3 spore syringes Golden Teacher, Pink Buffalo, and Ecuador, 12 wide mouth 1/2 pint canning jars, BRF, Horticultural Medium Grade Vermiculite, and Perlite for the Fruiting Chamber. Here is a base picture of my supplies


So after reading more and having some bad results after 7 days from inoculation, I believe I missed a step in sterilizing the jars. I punched a hole in the lids and positioned them upside down so the sharp part is facing down, then I covered them in tin foil and sterilized with a pressure cooker for 40 minutes. Unfortunately after they were cooling I read about someone putting masking tape over the holes of the substrate. I did no do this and when I took them out I noticed that there was a discoloration of the water, and the caps that I had extra and propped them up on looked like they had some vermiculite on them.


Ill take some pictures of my inoculated jars after 7 days, but there is little to none rizomorphic growth and I believe this to be the result of a lack of water, I heard people should have moisture on the sides of their substrate and there is none at all. My question is should I add sterilized water to the substrate and hope it starts to grow, or should I completely restart and call it a failure. Any help is appreciate and cheers!
-------------------- "Psychedelics are illegal not because a loving government is concerned that you may jump out of a third story window. Psychedelics are illegal because they dissolve opinion structures and culturally laid down models of behavior and information processing. They open you up to the possibility that everything you know is wrong." ― Terence McKenna
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OgreLokon
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Re: First Mycological Experiment! NeEd SomE Help [Re: Jalidone]
#19087577 - 11/04/13 09:47 PM (10 years, 2 months ago) |
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Do not add anything now. Don't worry about the masking tape, since you don't want to cover those holes anyway.
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silverstem
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Re: First Mycological Experiment! NeEd SomE Help [Re: Jalidone]
#19087582 - 11/04/13 09:47 PM (10 years, 2 months ago) |
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Quote:
Jalidone said: I read about someone putting masking tape over the holes of the substrate. I did no do this and when I took them out I noticed that there was a discoloration of the water, and the caps that I had extra and propped them up on looked like they had some vermiculite on them.
was there foil on them?
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Jalidone
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Re: First Mycological Experiment! NeEd SomE Help [Re: silverstem]
#19087616 - 11/04/13 09:56 PM (10 years, 2 months ago) |
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If you look up at the picture with the syringes on top of the substrates, they were put in the PC as shown. The foil completely covered the top but was somewhat lose as tin foil tends to be. When I was in my glove box to inoculate them I noticed a good amount of condensation on the underside of the foil (above my 3 inoculation holes) I'm uploading the pics of the jars shortly. Thanks for the quick response!!
-------------------- "Psychedelics are illegal not because a loving government is concerned that you may jump out of a third story window. Psychedelics are illegal because they dissolve opinion structures and culturally laid down models of behavior and information processing. They open you up to the possibility that everything you know is wrong." ― Terence McKenna
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silverstem
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Re: First Mycological Experiment! NeEd SomE Help [Re: Jalidone]
#19087676 - 11/04/13 10:09 PM (10 years, 2 months ago) |
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after inoculation i would put micropore tape(not masking tape) on top of all inoculation holes. it decreases the chances on contams making it to your dry verm layer if you used one.
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Jalidone
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Re: First Mycological Experiment! NeEd SomE Help [Re: silverstem]
#19087754 - 11/04/13 10:28 PM (10 years, 2 months ago) |
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ALright so heres a little update, I went to check on my inoculation room with some disappointing results, these are the jars after 7 days.


 So even with the low quality pic (can anyone tell me how in increase the quality that they show up with, they are taken in HD) you can see there is little to no growth, Once again i think this has to do with the lack of moisture because they seem very dry. I am currently mixing 8 more substrates and I will repeat the sterilization in the pressure cooker this time with micropore tape over the holes so that the water does not bubble in.
After note: I just opened one of the jars that did not show any colonization and the cake wasn't extremely moist but it was kind of spongy with a sense of moisture, how moist is the substrate supposed to be to promote growth? Whats more perplexing is that some of them have mycelium while others do not.
Edited by Jalidone (11/04/13 10:39 PM)
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Kagenical
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Re: First Mycological Experiment! NeEd SomE Help [Re: Jalidone]
#19087820 - 11/04/13 10:44 PM (10 years, 2 months ago) |
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87f is too hot. Get it between 74 and 80f at the absolute maximum.
Over 78f is asking for more contaminates.
Also, when doing BRF cakes, you don't want them wet. You want a damp mixture of substrate. If you followed the recipes properly, you should be fine.
1 week from innoc, well, IMHO, that's what BRF cakes should look like. I don't see anything wrong, except for perhaps the massive holes you hammered in the top of your jars.
Whenever I did BRF cakes, quite some time ago, I would make 4 small holes with a nail in the jar lids. About 4mm in diameter. I would then shoot in each of the holes, and cover each jar with 3 coffee filters and an elastic band. The foil is preventing gas exchange, which you don't want. I know coffee filters are a poor filtering medium, but they always worked well for me... And my BRF cakes always took 4-5 weeks to fully colonize at 75f.
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Giggle_Grower
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Re: First Mycological Experiment! NeEd SomE Help [Re: Kagenical]
#19087945 - 11/04/13 11:17 PM (10 years, 2 months ago) |
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Your jars look normal. Take the foil off put them.upright on a shelf andforget about them for like 3 wks. Tipping them on the side disturbs your dry verm barrier, which can lead to contams. And lose the incubator! Anything over 80 will help the comtams and your jars will produce a little heat of their own. You want your temps in the 70-75 range.
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Edited by Giggle_Grower (11/04/13 11:20 PM)
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Jalidone
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Re: First Mycological Experiment! NeEd SomE Help [Re: Kagenical]
#19088028 - 11/04/13 11:39 PM (10 years, 2 months ago) |
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I think you may have been mislead by the picture, here is a picture of the holes I punched, they are pretty much the smallest that my syringe would allow. I was mislead about the temperature and read that you should keep the inoculated jars 10 degrees higher than you plan to grow them in, I have since watched RR's video and I see that high 70's low 80's is best.
-------------------- "Psychedelics are illegal not because a loving government is concerned that you may jump out of a third story window. Psychedelics are illegal because they dissolve opinion structures and culturally laid down models of behavior and information processing. They open you up to the possibility that everything you know is wrong." ― Terence McKenna
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silverstem
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Re: First Mycological Experiment! NeEd SomE Help [Re: Jalidone]
#19088058 - 11/04/13 11:49 PM (10 years, 2 months ago) |
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you want to keep ur jars about 5 to 10 degrees below 75 to 80 only when there is alot of myc present. myc lets out its own heat. i keep my jars and plastic Tupperware at 68. i havent had a contam yet. but thats mostly because of my sterilization tek and my great spore syringe mixture.
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Jalidone
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Registered: 11/04/13
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Last seen: 9 years, 10 months
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Re: First Mycological Experiment! NeEd SomE Help [Re: silverstem]
#19088143 - 11/05/13 12:09 AM (10 years, 2 months ago) |
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Thanks everyone for the constructive criticism! I moved them to a place in my apartment that is 75 right now, it should be consistent which is nice. I only put them on their side for the purpose of a picture but I understand how that could disturb the dry vermiculite layer. I inoculated some Pink Buffalo and Ecuadorian tonight so ill keep you guys posted when I see some growth!
Edited by Jalidone (11/05/13 03:37 AM)
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