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OfflineOOKLA
Mr.Slippyfist

Registered: 01/09/03
Posts: 443
Last seen: 3 years, 7 months
Can DI water be used for spore syringes. *DELETED*
    #1908751 - 09/12/03 08:07 AM (13 years, 6 months ago)

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OfflineExtravagantDream
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Re: Can DI water be used for spore syringes. [Re: OOKLA]
    #1908923 - 09/12/03 10:38 AM (13 years, 6 months ago)

I believe class 100000 but it wouldn't be considered a cleanroom.
Measured particle size in micrometers (M)

Class Name...... 0.1M... 0.2M... 0.3M... 0.5M... 5M
1 (M 1.5).......... 35....... 7.5...... 3...... 1......... N/A
10 (M 2.5)........ 350..... 75....... 30..... 10....... N/A
100 (M 3.5)...... N/A..... 75....... 300... 100...... N/A
1000 (M 4.5)..... N/A..... N/A..... N/A.. 1,000.... 7
10000 (M 5.5)... N/A..... N/A..... N/A.. 10,000.. 70
100000 (M 6.5). N/A..... N/A..... N/A.. 100,000. 700



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InvisibleCow Shit Collector
Patty Poacher

Registered: 02/15/01
Posts: 1,959
Loc: Random Field
Re: Can DI water be used for spore syringes. [Re: OOKLA]
    #1909956 - 09/12/03 04:36 PM (13 years, 6 months ago)

Quote:

OOKLA said:
Also does anyone know if my particle counter is reading 5000 PPM, what class cleanroom does that make a glovebox? 




Your particle counter?
Lucky dog  :cool: :wink:


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OfflineOOKLA
Mr.Slippyfist

Registered: 01/09/03
Posts: 443
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Re: Can DI water be used for spore syringes. *DELETED* [Re: Cow Shit Collector]
    #1910823 - 09/12/03 09:37 PM (13 years, 6 months ago)

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OfflineExtravagantDream
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Re: Can DI water be used for spore syringes. [Re: OOKLA]
    #1910962 - 09/12/03 10:37 PM (13 years, 6 months ago)

Bastard.. got some good stuff.

What are you going to use the bunny suit for, I know they don't shed particles.. but if you arn't in a clean room there is no purpose. As for the glovebox, it should suffice. Done it with great success under a table in a room.

I was trying to figure out what DI was.. I've seen it before and I know I know what it is.. but I just can't remember. For syringes I generally use distilled.. but any should do as long as it has been sterilized.


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OfflineOOKLA
Mr.Slippyfist

Registered: 01/09/03
Posts: 443
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Re: Can DI water be used for spore syringes. *DELETED* [Re: ExtravagantDream]
    #1910987 - 09/12/03 10:44 PM (13 years, 6 months ago)

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Offlinejustsmurfy
little bluetwerp

Registered: 09/09/03
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Re: Can DI water be used for spore syringes. [Re: OOKLA]
    #1911359 - 09/13/03 01:01 AM (13 years, 6 months ago)

I worked in couple of different labs in grad school, mostly basic biotech, one did molecular genetics (splicing genes into bacteria and plants) and plant tissue culture (good sterile technique practice for me!). Most people used DI for everything- it was piped into every lab and many offices too, oddly enough. My advisor used it for tea- the local water was crappy. I used to smuggle it out for homebrewing (water chemistry changes all sorts of things in brewing and you can add minerals to get the right ions for your style of beer).

It was a big ass school and during my four years there and out of the maybe forty labs I saw, only one lab that used distilled instead of each building's piped in DI. They triple distilled tap water and then autoclaved it. I asked about the difference between DI and distilled water- apparently DI is _much_ cheaper to make, thus the odds-on favorite. There where minor differences in quality- both retained much of the volatile contaminants and very few of the mineral contaminants. Apparently the mineral contaminants where of a much greater concern- no ions, no new metal-containing proteins could be made by any bacteria or fungi.

__So they were, for a serious research institute, considered equivalent.__

I can't se any reason that you can't use DI.

I must stress that (as you probably already realize) that DI is not necessarily sterile. PC it or autoclave it, if you got one of those babies with the bunny suits.

-Just Smurfy


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Offlinehenryp
Refugee from the Group W bench

Registered: 12/17/01
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Yes [Re: OOKLA]
    #1911504 - 09/13/03 02:11 AM (13 years, 6 months ago)

DI H20 will work fine for syringes.

Autoclave it if you're worried about contams.....


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OfflineExtravagantDream
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Re: Yes [Re: henryp]
    #1912768 - 09/13/03 03:21 PM (13 years, 6 months ago)

De-ionized.. sounds like the charge is taken out.  :confused:


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