|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
|
TiT revisited
#19059110 - 10/30/13 06:23 PM (10 years, 3 months ago) |
|
|
Here's my TiT (tub-in-tub incubator).
I haven't used a TiT in a long time.
Now that I have some experience and more knowledge under my belt, I recently decided to go back to using one.
This is my first trial with it.
I've been monitoring it for several days since I made it. The temperature inside stays between 79.8F and 80.5F.

In this first run, I have 7 quarts of Rye Berries inoculated with agar 4 Petris with MS from a syringe 2 Petris with MS from a print 1 Petri with a transfer from the same agar I used in the jars.
This was all done in a SAB (Still Air Box)
As things develop, I will post results.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
|
SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
|
|
Update:
After 48hrs from OP
The wedges in the rye seem to be starting to jump to the grains. About what I'd expect in a short time.
The agar transfer is growing well. It's gone from the size of a small short grain rice to about 5/8".
I can't get a good pic because The Petris all have condensation. They did not have condensation before they went in the TiT.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
|
Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 3 hours, 31 minutes
|
|
I just made me a titi for the winter months. I heat with a woodstove so the back bedroom where iI keep my jars gets pretty cold. My grain jars have been colonizing very quick. About 8 to 10 days for g2g jars
|
blueconfusion
Strangest



Registered: 12/14/12
Posts: 1,727
Last seen: 14 hours, 30 minutes
|
|
been thinking about going back to a TiT myself but i'm slowing down for a minute i'm sure once i pick back up i'll be full on for a TiT. do you use clear tubs been kinda curious if anyone has done this considering they are pp5 and work perfectly fine... since so many ppl swear by ambient light during colonization this would be the perfect balance clear tubs with heating right?
|
PsiLisaBin
Stranger<<<Strangest


Registered: 07/11/13
Posts: 128
|
|
damn, so incubating is what's hot in the streets of the shroomery these days, huh? seen a number of threads on this topic here lately....
i first started lurking on here last winter as i was first getting started, and was thinking about building one of these but everyone swore against it......i actually don't think it's a bad idea at all to do this when it's winter-time and you can't afford to have the heat on at 80 for four months in a row
|
blueconfusion
Strangest



Registered: 12/14/12
Posts: 1,727
Last seen: 14 hours, 30 minutes
|
|
i wasn't for TiT for a while simply because i had so many problems with mine namely the fact i didn't know what i was doing and contaminated everything... now that i have a better grasp and am not blaming something that was not actually the problem i'm thinking of it trying again...
|
SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
|
|
It's Halloween time so black tubs are cheap.
If it's cold, you may benefit greatly from one. Mycelium does colonize faster at 80F than at 70F. And definitely faster than at 60F.
My limited testing in regards to light have not shown a difference either way. I don't intentionally colonize in the dark or light.
In regards to incubators and light, there is a lot of "quoting" on these forums, but few "quoters" have actually produced their own evidence and/or data.
Incubators are not necessary in most cases, but they are not outdated. Do your own experiments and report back. I like looking at successful peoples pics and doing what they do. Fuck the quoters.
I will keep this thread updated as things develop.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
|
SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
|
|
Quote:
Stromrider said: I just made me a titi for the winter months. I heat with a woodstove so the back bedroom where iI keep my jars gets pretty cold. My grain jars have been colonizing very quick. About 8 to 10 days for g2g jars

Quote:
blueconfusion said: i wasn't for TiT for a while simply because i had so many problems with mine namely the fact i didn't know what i was doing and contaminated everything... now that i have a better grasp and am not blaming something that was not actually the problem i'm thinking of it trying again...
Sounds like my story. Turns out that TiTs don't cause contams, I cause contams.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
|
Stromrider
This must be the place



Registered: 06/02/13
Posts: 7,338
Loc: Dept of know what I'm say...
Last seen: 3 hours, 31 minutes
|
|
Quote:
PsiLisaBin said: damn, so incubating is what's hot in the streets of the shroomery these days, huh? seen a number of threads on this topic here lately....
i first started lurking on here last winter as i was first getting started, and was thinking about building one of these but everyone swore against it......i actually don't think it's a bad idea at all to do this when it's winter-time and you can't afford to have the heat on at 80 for four months in a row 
A lot of people just tell noobs not to incubate because they do dumb shit like put their jars in a tub on top of a heating pad cooking the bottom of the jars
|
Rubestoad
Stranger


Registered: 09/13/13
Posts: 231
Loc: your mamas porch. sunrise
Last seen: 8 years, 3 months
|
|
I'm one of those newbs kinda ... I have a cooler with a terrarium heater on the top side so it warms the cooler but isn't drying the air inside it stays at 79.9 inside is this ok ?
-------------------- Go placidly amid the noise and haste, and remember what peace there may be in silence.
|
SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
|
|
The trick to TiTs is having steady and even heating.
But I really didn't intend on turning this in to a "how to".
I just wanted to allow people to track my results.
The link in the OP shows how I made mine.
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
Edited by SpitballJedi (11/01/13 07:51 PM)
|
SpitballJedi
Ancient Astronaut



Registered: 10/13/12
Posts: 8,598
Loc: Nibiru
|
|
Update
3days 15hours from OP
1 print contaminated. Un-salvageable.
1 print no growth.
1 jar trich
6 jars seem healthy and colonizing.
Neither syringes shows growth.
The transfer had a smooth lump on one side. I made 2 new transfers.
 
I made a new print agar
-------------------- The Basics A little civility goes a long way The Noob Forum The Hammock Hangers' Forum
|
|