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Offlineeelrd72
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my first agar project
    #18945040 - 10/07/13 02:10 PM (10 years, 4 months ago)

i started this project messing up. Thought I was taking the good away from the contaminated part of dish. Then found out.WRONG. Not suppose to take the contaminate away from the good mycelium in front of flow hood. So I cleaned up the area with alcohol and lysol. At lunch I took one of my dishes made from a print. Had the hardest time figuring out how to use the inoculation loop. There was no directions that came with it. Im pretty sure I scraped way too much. Also scalpel did not have instructions with it either. Took me what seemed like forever to figure out the blade clipped on ..instead of trying to squeeze it in the middle. Anyways..cut away what appeared to be the rope fingerlike projections. Cut 5 pieces and put in 5 dishes. Also didnt know how to use that plastic wrap. Found a Youtube video on it.


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OfflineStromriderM
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Re: my first agar project [Re: eelrd72]
    #18945080 - 10/07/13 02:22 PM (10 years, 4 months ago)

Wow a couple of those plates are disastrous! :whatdidyoudo:


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Offlineeelrd72
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Re: my first agar project [Re: Stromrider]
    #18945160 - 10/07/13 02:39 PM (10 years, 4 months ago)

my first time....probably because I scraped way tooo much..on there. I couldnt find a good example of going from print to plate. In fact not even sure about the inoculating loop I bought from sponser.


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OfflineStromriderM
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Re: my first agar project [Re: eelrd72]
    #18945181 - 10/07/13 02:44 PM (10 years, 4 months ago)

You don't want to ever scrape spores onto a dish. You want to flame sterilize the loop and then cool it in the receiving dish. When you do that some agar will be on the end of the loop allowing you to just touch your print and get a few spores to stick to the end :thumbup:


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Offlineeelrd72
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Re: my first agar project [Re: Stromrider]
    #18945349 - 10/07/13 03:21 PM (10 years, 4 months ago)

Thanks..Im going to try to do a few more plates tonite the way that you described it. I know it all sounds easy but once i go to do it..the blower is blowing the print, im trying to hurry, didnt seem like it was picking up anything. Even tho doesnt look like its picking up anything, now I know it is.


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OfflineStromriderM
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Re: my first agar project [Re: eelrd72]
    #18945500 - 10/07/13 03:59 PM (10 years, 4 months ago)

:thumbup:


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Offlineeelrd72
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Re: my first agar project [Re: Stromrider]
    #18954740 - 10/09/13 02:42 PM (10 years, 4 months ago)

i let my flow hood run for about 6 hours. Then using sterile technique I think I figured out how to twist the metal thread on the inoculation loop to make it sturdier and have a loop at the end. After flaming it, I dipped in the receiving dish to cool and scraped alittle and put in the center. On those first ones...I scraped a bunch up and kinda dusted the plate. yeah I know..Now. Thats why the plates look so messed up. I put these right in the center only opening the lid for seconds at a time. Then I wrapped up and put back into ziplock baggie filled with air from the flowhood. Will give 5 days before taking a look. Will post pics of those then. Thanks for the help.


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Offlineeelrd72
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Re: my first agar project [Re: eelrd72]
    #18984918 - 10/16/13 05:55 AM (10 years, 4 months ago)

adding some more pics of my agar project. I have these small saucer size plates i decided to go ahead and try to isolate some GT. I put two on each plate . i went ahead and had 4 jars ready so I threw together a monotub also..


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OfflineMastaBlastar
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Re: my first agar project [Re: eelrd72]
    #19044720 - 10/28/13 08:43 AM (10 years, 3 months ago)

I like this thread, been lurking it and decided to post as I am about to start agar myself.  Updates?  I wanna see how those tubs did, looking to do side by side MS/isolate tests soon. 
  Got 12 plates (plastic, single zone) and some parafilm.  Looking around for the best deal on an agar mix rather than getting the individual components.  Also, I am going to try it in my SAB as that is all I have, at least I went all out on it. The gloves are not attached yet in that pic and I took that black shelf out and just use the white shelf thats in there and another identical one.  I set the jars on one side and the bags on the other and it works great.  I can do 4 bags at a time in it rather than have 4 bags take up to 2+ hours for time taken to clean and re-wet the box with fresh bleach water and trying to do large myco bags in a 105qt tub is a BITCH to say the least, lost 2 bags because the tub was too short and I hit the top and the tub came off the lid. 
    Also, I have some of the stuff for my flowhood and if agar in a SAB doesn't go so well(bags and jars work GREAT in it, so don't see why agar wouldn't) but probably will now be late nov-dec before I start building it. 
How are your tubs doing?  PICS MAN PICS!!!


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Everything I have said, may say, will say, am thinking about saying and/or thinking/typing/dreaming/writing is in all likelyhood made up and has no factual basis in reality whatsoever, and is likely all plagiarized and copy pasted straight from someone else, so get mad at them .  Just a warning


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Offlineeelrd72
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Re: my first agar project [Re: MastaBlastar]
    #19053286 - 10/29/13 06:01 PM (10 years, 3 months ago)

no pics yet...noticed today..about 10 or so pins...just starting..will post pics..as soon as I can..bout nother week


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InvisibleNimpo
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Re: my first agar project [Re: eelrd72]
    #19053410 - 10/29/13 06:28 PM (10 years, 3 months ago)

OP if you are gonna keep posting pictures, make sure your focus is right! 95% of the plate close ups are all out of focus.

Other than that, good work :thumbup:


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Offlineeelrd72
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Re: my first agar project [Re: Nimpo]
    #19057972 - 10/30/13 02:36 PM (10 years, 3 months ago)

yeah ..sorry about the focus...im all nervous about the contamination while im working in there...i will get some better pics..  I got some real good ones of contaminations...Ive counted more than 20 or so Pins..today..cant get a good photo on the monotub yet..still real steamy in there


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Offlineeelrd72
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Re: my first agar project [Re: eelrd72]
    #19078883 - 11/03/13 05:49 AM (10 years, 3 months ago)

took couple pics of How Not To Do Things...was thinking of copying Franks Getting Shit done.....to my Own How I Fuck My Shit Up....and a few of how to get things done.
I dont fuck up everything tho

trying to take a better pic. the monotub is looking good...need to read how frank gets things done again...


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OfflineStromriderM
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Re: my first agar project [Re: eelrd72]
    #19079132 - 11/03/13 07:35 AM (10 years, 3 months ago)

Wow!  Those are some of the worst looking jars I've ever seen!


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OfflineMastaBlastar
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Re: my first agar project [Re: Stromrider]
    #19079388 - 11/03/13 09:08 AM (10 years, 3 months ago)

i have had worse.  well...maybe, those are pretty gd bad.  Had a couple black ones, ii think those are worse from a health danger factor.


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Offlineeelrd72
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Re: my first agar project [Re: Stromrider]
    #19081311 - 11/03/13 04:41 PM (10 years, 3 months ago)

those jars have been sitting for quiet awhile now. I just wanted to see what they grew into. Threw out just after the pic. The messed up agar dishes also..been watching how they grow also.


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Offlineeelrd72
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Re: my first agar project [Re: eelrd72]
    #19094099 - 11/06/13 05:25 AM (10 years, 3 months ago)

here are some updated pics of the monotub 


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OfflineMidnight Cyclone
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Re: my first agar project [Re: eelrd72]
    #19094252 - 11/06/13 07:33 AM (10 years, 3 months ago)

Quote:

eelrd72 said:





Quote:

Stromrider said:
Wow!  Those are some of the worst looking jars I've ever seen!




Dude, you've got every contamination in the books! :lol:

I do the same thing occasionally, with watching what the contaminated jars turn into. Just watch that black mold... that shit is nothing to take lightly.

Quote:

eelrd72 said:
 




Happy to say, nice! :thumbup:


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Offlineeelrd72
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Re: my first agar project [Re: Midnight Cyclone]
    #19094320 - 11/06/13 08:16 AM (10 years, 3 months ago)

thanks for the input..yeah..i just kept it ...to see what would happen..I actually threw them in my neighbors garbage on Monday at 4:30 am cause the trash guy comes at 7am.  Hopefully I will not have to deal with as much contaminated rye..as to working with agar now.


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OfflineMastaBlastar
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Re: my first agar project [Re: eelrd72]
    #19098497 - 11/07/13 12:10 AM (10 years, 3 months ago)

Quote:

eelrd72 said:
here are some updated pics of the monotub 



:bearbreakdance::wooawesome: :sherm:  :fappage:  :rocketcrotch:  Nicely done sir!


--------------------
Everything I have said, may say, will say, am thinking about saying and/or thinking/typing/dreaming/writing is in all likelyhood made up and has no factual basis in reality whatsoever, and is likely all plagiarized and copy pasted straight from someone else, so get mad at them .  Just a warning


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