|
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
My first atempt rye berry transfer to WBS
#19075670 - 11/02/13 03:26 PM (10 years, 2 months ago) |
|
|
 Ill report the progress weekly if it takes.Just trying diffident methods to find out what works best for me what are some of the best methods for large scale production in the northern climates??any input is welcome
|
CAP_TURTLE
Adventurer



Registered: 03/11/05
Posts: 1,135
Last seen: 5 years, 8 months
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19077445 - 11/02/13 10:16 PM (10 years, 2 months ago) |
|
|
Why not just transfer to more rye berries? Also are you doing actives or gourmets :P
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
Re: My first atempt rye berry transfer to WBS [Re: CAP_TURTLE]
#19079709 - 11/03/13 10:45 AM (10 years, 2 months ago) |
|
|
gourmet. Just trying out all the tek to find what works best for me,and wild bird seed is a pretty accessible around here.Rye berries were fairly simple and i wanted to see if WBS tek was any quicker in the prep time so far its just about the same... My next trial tek will be a few mini straw bales inoculated with the pearl oyster WBS in the pics.I will also try the rye berries on the straw to see which one colonizes faster.
Edited by eagel774 (11/03/13 10:47 AM)
|
CAP_TURTLE
Adventurer



Registered: 03/11/05
Posts: 1,135
Last seen: 5 years, 8 months
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19081749 - 11/03/13 06:09 PM (10 years, 2 months ago) |
|
|
I don't think your going to see a noticable difference in the wbs and rye. I recently switched to rye and it is almost fool proof and I like it way better. Working on other conditions will affect your colonization more than anything I expect.
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
Re: My first atempt rye berry transfer to WBS [Re: CAP_TURTLE]
#19084263 - 11/04/13 06:07 AM (10 years, 2 months ago) |
|
|
I did 11 jars of rye berries and only 4 took. the first I injected distilled water into a plug spawn bag and transferred that to the jars. After five or so days i noticed only a few jars started to colonize.So inpatient as i can be at times,loaded up the other jars with a single plug spawn in each jar(under sanitary conditions of course) and they took off quick and then just fell flat.once the berries take off like that should i agitate the berries or WBS to speed up the process or is it better to just let it be??
|
CAP_TURTLE
Adventurer



Registered: 03/11/05
Posts: 1,135
Last seen: 5 years, 8 months
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19084396 - 11/04/13 07:47 AM (10 years, 2 months ago) |
|
|
Interesting. Is it possibly a little on the cool side in the room you colonize in? It's possible that when you made the mycelium water you disturbed the mycelium a little more than you did removing a single dowel so there was less recovery time needed with the dowel. If you did the mycelium water correctly and you didn't see any contams after 5 days you likely just needed a little more time with the mycelium water. I usually shake my jars sometimes twice depending on how good my dispersion is as I like to fit as much into my jars as I can and still shake at all. Some might recommend against this. idk. I know it's commonly recomended to shake once around 30 percent or so. With oysters and other rapid colonizers I sometimes wonder if it wouldn't just be faster to let them be and not disturb at all but I also typically use more than one initial inoculation point via grains or multiple agar wedges so it's spread out to an extent anyways. A final word of advice: Have Patients. Fully Observe. And Learn.
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
Re: My first atempt rye berry transfer to WBS [Re: CAP_TURTLE]
#19084407 - 11/04/13 07:53 AM (10 years, 2 months ago) |
|
|
I think i shook them too much.the smaller one in the pic worked great and i only shook em up a few times because i could see them better in the smaller jar. im just going to let em go for another week and if they don't rebound ill take it as a loss.4 out of 11 on my first attempt with rye is not bad..I think... thank for the input cap
Edited by eagel774 (11/04/13 07:54 AM)
|
Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 8 hours
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19084418 - 11/04/13 07:57 AM (10 years, 2 months ago) |
|
|
It would be better to start with a good culture, agar or lc syringe, and not plug spawn. Most plugs by the time you get them have had the culture expanded pretty far already, that's probably it's stalling once you get it on grain (that, or it's contaminated). AV explains the plug thing a little better than I can, in this thread if you read through it.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
|
CAP_TURTLE
Adventurer



Registered: 03/11/05
Posts: 1,135
Last seen: 5 years, 8 months
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19084422 - 11/04/13 07:57 AM (10 years, 2 months ago) |
|
|
Yeah I definitely think you need to just let them go for some time. You will learn the cycle and be able to hone your skills. Shaking them every couple days or two with no significant growth in between is just further shocking the mycelium and not allowing any time for recovery. Oh by the way, what species are you doing? Many are speedy colonizers but shiitakes for me are much slower than anything else I grow.
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
Re: My first atempt rye berry transfer to WBS [Re: CAP_TURTLE]
#19185728 - 11/25/13 07:45 AM (10 years, 2 months ago) |
|
|
As of 11/25/13 the WBS & rye berries (i did a week later) are fully colonized with pearl oyster and ready to transfer to whatever sub i can use (gonna try cornstalks as i have 70 acres of them). It took approximately 28 days to colonize the WBS fully and 18 days to colonize the rye berries. I did notice that the filter disk i used let alot of excess moisture escape leaving the top berries dry and contaminated looking.So i covered up about 90% of the filter disk to reduce moisture loss,and am waiting to see if the oysters will recover the top berries,or the alternate,contamination!! Fore the WBS i used a syringe to inoculate all the jars. The berries were inoculated from plug spawn scrapings and did colonize a week faster. The WBS liquid transfer seams to be much stronger and fuller looking but took a little longer to recover and colonize. I personal think rye berries are much faster than WBS especially with prep times.I think the colonization rates would be close to the same if i had inoculated both (WBS & Rye berries) with the same procedures.Two jars WBS & berries are going into sawdust bags.Is this enough to transfer to a 5# block?

|
Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 8 hours
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19189321 - 11/25/13 10:07 PM (10 years, 2 months ago) |
|
|
Quote:
eagel774 said: As of 11/25/13 the WBS & rye berries (i did a week later) are fully colonized with pearl oyster and ready to transfer to whatever sub i can use (gonna try cornstalks as i have 70 acres of them). It took approximately 28 days to colonize the WBS fully and 18 days to colonize the rye berries. I did notice that the filter disk i used let alot of excess moisture escape leaving the top berries dry and contaminated looking.So i covered up about 90% of the filter disk to reduce moisture loss,and am waiting to see if the oysters will recover the top berries,or the alternate,contamination!! Fore the WBS i used a syringe to inoculate all the jars. The berries were inoculated from plug spawn scrapings and did colonize a week faster. The WBS liquid transfer seams to be much stronger and fuller looking but took a little longer to recover and colonize. I personal think rye berries are much faster than WBS especially with prep times.I think the colonization rates would be close to the same if i had inoculated both (WBS & Rye berries) with the same procedures.Two jars WBS & berries are going into sawdust bags.Is this enough to transfer to a 5# block?
Are you asking if a single jar of spawn is enough for a 5lb block? Should be more than enough, I use a full jar for every block but most people tell me that's way more than you need.
28 days seems like a long time for a jar of oyster spawn to colonize, but maybe I'm wrong I don't really keep records
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
Re: My first atempt rye berry transfer to WBS [Re: Forrester]
#19190756 - 11/26/13 07:51 AM (10 years, 2 months ago) |
|
|
I read somewhere that to much grain spawn in the blocks will reduce yeilds..has anyone ever tried cornstalks instead of straw?
|
Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 8 hours
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19194533 - 11/26/13 10:36 PM (10 years, 2 months ago) |
|
|
Quote:
eagel774 said: I read somewhere that to much grain spawn in the blocks will reduce yeilds..has anyone ever tried cornstalks instead of straw?
I haven't but I've thought of it, I drive by acres and acres of them every day that go unused. Oysters will grow on anything so definitely give it a go. I'm sure someone has done it.
And as far as I know too much grain spawn will not reduce yields. It might make the substrate more nutritious, which might make it take longer to colonize and fruit, but it should only help yields.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
Re: My first atempt rye berry transfer to WBS [Re: Forrester]
#19221993 - 12/03/13 12:41 PM (10 years, 1 month ago) |
|
|
Ive got two block of corn cobs and aspen bedding going with oysters as of 11/29. I pasteurized at 180 for about 2 hrs let it cool and dumped a qt a piece of WBS. Does this look like too much moisture???
 Would this qualify as a mini mono-tube
Edited by eagel774 (12/03/13 12:46 PM)
|
Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 8 hours
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19224857 - 12/03/13 11:05 PM (10 years, 1 month ago) |
|
|
Looks good from here, but hard to tell. Seems to be doing well for the time period given. If after a while the whole thing is colonized except some parts around the bottom that look too wet, then you'll know.
180's kinda high for pasteurization, isn't it?
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
Re: My first atempt rye berry transfer to WBS [Re: Forrester]
#19225405 - 12/04/13 05:56 AM (10 years, 1 month ago) |
|
|
I was thinking that the aspen bedding would need a little higher temps so i kept it at 180. Is it bad to pasteurize at higher temps? its my first time so I'm not sure.
|
Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 8 hours
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19225743 - 12/04/13 08:42 AM (10 years, 1 month ago) |
|
|
I might be a little off as I never do real pasteurization, but temps should remain somewhere between like 140-160f. The whole point is you want certain bacteria/organisms to survive that will help, when you go to 180f you might be killing some/all of those beneficial organisms by partially sterilizing.
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
Re: My first atempt rye berry transfer to WBS [Re: Forrester]
#19230169 - 12/05/13 07:02 AM (10 years, 1 month ago) |
|
|
I see..Ill keep the temps down next run and see what happens.I was surprised to see them colonize so quickly on something that's not sterilized.
|
Forrester
aspiring sociopath


Registered: 02/05/13
Posts: 9,351
Loc: Northeast USA
Last seen: 25 days, 8 hours
|
Re: My first atempt rye berry transfer to WBS [Re: eagel774]
#19230245 - 12/05/13 07:52 AM (10 years, 1 month ago) |
|
|
Yeah oysters are pretty tough so it might not matter anyway, if it's colonized you're probably fine!
-------------------- Repugnant is a creature who would squander the ability to lift an eye to heaven, conscious of his fleeting time here. ------------------- Have some medicinal mushrooms and want to get the most out of them? Try this double extraction method.
|
eagel774
Handsom Stranger


Registered: 11/01/13
Posts: 78
Loc: Michigan
Last seen: 6 months, 6 days
|
Re: My first atempt rye berry transfer to WBS [Re: Forrester]
#19249037 - 12/09/13 07:40 AM (10 years, 1 month ago) |
|
|
My first flush ever this is cool!!....How long do i let them go before harvesting??
|
|