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InvisiblePsiLisaBin
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Registered: 07/11/13
Posts: 128
sudden cobweb in jars
    #19075180 - 11/02/13 01:32 PM (10 years, 2 months ago)

Please help me come up with a reason why this may have happened to me...

Had some jars I inoculated with an agar wedge on 10/19.  They had been fine until I looked at them last night and I noticed three of them have a good amount of cobweb in there (i'm positive that's what it is).

Now usually, if I did a sloppy inoculation (esp. with an agar wedge inoc. where you have to crack open the receiving jar lid) the contam would show up within a few days of the inoculation.  It's been 2 weeks since the inoculation and things were cool until yesterday - and I know they were ok because I do glance at them everyday, just a visual check, I'm not handling them and disturbing them.  So do you agree that the contam didn't happen during the inoculation, but some time after?  2 weeks is too long for cobweb to sprout up if it was introduced then, since we all know how quickly it spreads - so I'd have to assume it somehow crept in within the last day or two.  But then what else could cause this, how could a contam get through the jar lid like that after the fact?  Lemme know if there's any other details I can share - these are quart jars of wbs.    Sucks cuz they were looking GOOD up til last night, was going to shake them yesterday :mad2:


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OfflinecronicrFacebook
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Re: sudden cobweb in jars [Re: PsiLisaBin]
    #19075206 - 11/02/13 01:41 PM (10 years, 2 months ago)

its happens, sometimes doest show up till some colonization takes place and drives co2 levels up, whiich is y it doesnt normally show its face on grain and mostly in casing layers. just my guess though


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InvisiblePsiLisaBin
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Re: sudden cobweb in jars [Re: cronicr]
    #19075222 - 11/02/13 01:46 PM (10 years, 2 months ago)

ok, so bottom line is this must have happened during inoculation?

is it like totally impossible for contaminant spores to get in through the sfd?  i know it provides gas exchange, so if a gas can move through the filter disc could outside spores also come in through it? i've always wondered that, but had been having a really good run until yesterday.......


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InvisiblePirateSwazey
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Re: sudden cobweb in jars [Re: PsiLisaBin]
    #19075276 - 11/02/13 01:58 PM (10 years, 2 months ago)

must have been during your inoculation...the filter discs are designed to only let air move through, and not larger particles like spores. i have heard of a wicking effect that can take place if the filter gets wet and bacteria hitches a ride into the jar through the moisture. i've had cobweb randomly take over a whole jar in a similar situation, except it was from a g2g transfer and not an agar wedge. all of the other jars from that g2g were fine except the one with overnight cobweb explosion which happened almost a week after the transfer. :shrug:


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OfflinecronicrFacebook
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Re: sudden cobweb in jars [Re: PsiLisaBin]
    #19075286 - 11/02/13 02:00 PM (10 years, 2 months ago)

filters do there job but what we call sterilized jars arent, we just buy ourself a window of oppurtunity but i would say yes probly during inoculation


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Invisiblejoiningheads
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Registered: 05/05/13
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Re: sudden cobweb in jars [Re: PsiLisaBin]
    #19075490 - 11/02/13 02:45 PM (10 years, 2 months ago)

Quote:

PsiLisaBin said:
ok, so bottom line is this must have happened during inoculation?

is it like totally impossible for contaminant spores to get in through the sfd?  i know it provides gas exchange, so if a gas can move through the filter disc could outside spores also come in through it? i've always wondered that, but had been having a really good run until yesterday.......




I don't think there's a "bottom line" but one thing I do know is that a spore doesn't have to germinate immediately.  It could have entered during inoc and not germinated until CO2 was high and it made contact with nutrients.
Synthetic filter discs are ideally 0.3 microns, and spores are several microns, so in an ideal world, it shouldn't get through.  Filter disc material (thru cutting or poor manufacturing) could however allow contams through. 
Molecules of O2 and CO2 are on the order of angstroms, which are 10,000 times smaller than microns. Big difference.


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InvisiblePsiLisaBin
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Registered: 07/11/13
Posts: 128
Re: sudden cobweb in jars [Re: joiningheads]
    #19075579 - 11/02/13 03:06 PM (10 years, 2 months ago)

Quote:

joiningheads said:
Quote:

PsiLisaBin said:
ok, so bottom line is this must have happened during inoculation?

is it like totally impossible for contaminant spores to get in through the sfd?  i know it provides gas exchange, so if a gas can move through the filter disc could outside spores also come in through it? i've always wondered that, but had been having a really good run until yesterday.......




I don't think there's a "bottom line" but one thing I do know is that a spore doesn't have to germinate immediately.  It could have entered during inoc and not germinated until CO2 was high and it made contact with nutrients.
Synthetic filter discs are ideally 0.3 microns, and spores are several microns, so in an ideal world, it shouldn't get through.  Filter disc material (thru cutting or poor manufacturing) could however allow contams through. 
Molecules of O2 and CO2 are on the order of angstroms, which are 10,000 times smaller than microns. Big difference.




thanks, that makes sense, you are a good explainer of things seriously :thumbup:


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