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Invisiblejpack666
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Registered: 10/01/13
Posts: 484
Print to agar vs seringe to agar?
    #19073097 - 11/02/13 12:34 AM (10 years, 2 months ago)

If one makes his own prints and starts his cycles with agar to grain transfers...

What would an expert do?

Would he go from print to agar (I myself wouldn't know how)?
Or would he add an extra step, make a seringe, and then go to agar?

I've searched a lot for this answer and nothing yet, sorry if it's on the forum and I haven't found it.

It's just been picking my mind for awhile now, if starting cultures from agar and ending them with prints, would the seringe become obsolete? And if not, why? I mean an expert wouldn't add an extra step unless it made a considerable difference no?

Ps: found this thread http://www.shroomery.org/forums/showflat.php/Cat/0/Number/1154400
Apparently a tek to make thousands of plates from one print?!?! But couldn't find the tek anywhere... Anyone knows about it?

Thanks for the help


--------------------
"There's a negative and a positive to everything."  :mushroom2:

For more information, RogerRabbit's Website/Videos


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OfflinePussyFart
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Re: Print to agar vs seringe to agar? [Re: jpack666]
    #19073187 - 11/02/13 01:01 AM (10 years, 2 months ago)

I have done both, and both work just fine.

That being said, I would not go thru the hassle of making a syringe for the sole purpose of knocking up plates.

Just scrape spores with a sterile loop to the agar.

Less chance of contams, and less hassle.

This is just my opinion.


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OfflineMacMerdin
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Re: Print to agar vs seringe to agar? [Re: jpack666]
    #19073882 - 11/02/13 07:37 AM (10 years, 2 months ago)

Quote:

jpack666 said:
if starting cultures from agar and ending them with prints, would the seringe become obsolete? And if not, why?




The syringe is still useful.  You can grow out a culture on agar until you think it is clean enough or no more sectoring is occuring.  Take a syringe, fill it with sterile water (same way we sterilize them to put spores into), squirt some sterile water onto the mycelium grown agar, scrape the mycelium to get it broken up and then pull the psuedo-liquid culture back into the syringe.  Do all this under a hood or in a SAB.

It's a good way to clean a dirty print and still be able to use a syringe.  It's how I cleaned wild Pan cinctulus spores for my current experiments.

But, if you are just looking to do grains and skip the PF tek all together, then like Hacker said, why use the extra step in making a syringe?  Just put spores to agar and use wedges to inoculate the grains.

Hope we helped.


--------------------
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Edited by MacMerdin (11/02/13 07:58 AM)


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OfflineStromriderM
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Re: Print to agar vs seringe to agar? [Re: MacMerdin]
    #19073908 - 11/02/13 07:45 AM (10 years, 2 months ago)

Definitely print to agar. It's hard to control how many spores your putting on the plate with a syringe. Also like hacker said it's just an unnecessary step


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InvisibleSpitballJedi
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Re: Print to agar vs seringe to agar? [Re: jpack666]
    #19073988 - 11/02/13 08:11 AM (10 years, 2 months ago)

As far as spores are concerned, making syringes become an unnecessary step when inoculating agar.

If you have spore syringes, use them, but don't bother making a syringe from spore print just to inoculate agar.


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InvisibleViolet
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Re: Print to agar vs seringe to agar? [Re: SpitballJedi]
    #19074131 - 11/02/13 08:58 AM (10 years, 2 months ago)

The above is correct.

I'll add that combining spore solution and agar often favors bacteria that may otherwise not appear.  Not sure of entirely why this is, but a spore syringe that contam's bacteria on agar may somehow do just fine on grains.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

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Invisiblejpack666
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Re: Print to agar vs seringe to agar? [Re: Violet]
    #19074710 - 11/02/13 11:34 AM (10 years, 2 months ago)

Thanks for all your answers everyone. Loving the learning thanks to you all.

There is so much reading to do. And planning (my friend realized this after screwing up his first grow attempts probably cause he was doing it all wrong and not reqding the forum)

...so this is great, like hacker said... One less step... And also with that last remark... It all makes a lot of sense.

Thanks again


--------------------
"There's a negative and a positive to everything."  :mushroom2:

For more information, RogerRabbit's Website/Videos


Edited by jpack666 (11/02/13 11:36 AM)


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OfflinePussyFart
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Re: Print to agar vs seringe to agar? [Re: Violet]
    #19075048 - 11/02/13 01:01 PM (10 years, 2 months ago)

Quote:

Violet said:
The above is correct.

I'll add that combining spore solution and agar often favors bacteria that may otherwise not appear.  Not sure of entirely why this is, but a spore syringe that contam's bacteria on agar may somehow do just fine on grains.



I have to say, I have noticed this as well.

I thought it was just me....lol.


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InvisibleViolet
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Re: Print to agar vs seringe to agar? [Re: PussyFart]
    #19075210 - 11/02/13 01:43 PM (10 years, 2 months ago)

It's one of the advantages that caused me to start making grain petris!  Agar-style outward growth with a spore solution, just 2-3 drops which don't roll around or turn into bacteria blotch, and countless easy tweezer transfers!  For that matter if they grow out clean they can easily be broken-up for a poured g2g like any other.

Quote:

Notahacker420 said:
Quote:

Violet said:
I'll add that combining spore solution and agar often favors bacteria that may otherwise not appear.  Not sure of entirely why this is, but a spore syringe that contam's bacteria on agar may somehow do just fine on grains.



I have to say, I have noticed this as well.

I thought it was just me....lol.



I've read RR say so much as well.
In my opinion, these are the sort of things that can plainly show the difference between real growers and community copycats, that only the real growers can be aware of.  Anyone who tries spore solution to agar enough times is likely to come to the same realization.  Again IMO, giving advice regarding spore solution to agar other than "Solution to agar sucks" is like giving advice regarding LCs other than "LCs suck"


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Edited by Violet (11/02/13 03:13 PM)


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Invisiblejpack666
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Re: Print to agar vs seringe to agar? [Re: Violet]
    #19075527 - 11/02/13 02:53 PM (10 years, 2 months ago)

Hey hacker... Do you need to do isolations/petri transfers to get plates like on your signature. Or do you sometimes get lucky and just use one print, innoc like 10 plates and they all turn out without contams? (In reality, I understand this would be like wasting a print but in theory, one might do it if he's innoculating hundreds of plates at once and then making thousands of babies from them.... Like commercial style maybe)

Although, I beleive from your picture that you have one "mother plate" and all the others are its "babies" so that leads me to think that the mother plate may have come from a few isolations...

(violet with her clean prints in vitro tek just got me thinking... How exactly unclean are prints usually? When you drop spores from the print on the agar, what are the probabilities of having contams and needing an isolation?)

Thanks


--------------------
"There's a negative and a positive to everything."  :mushroom2:

For more information, RogerRabbit's Website/Videos


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InvisibleViolet
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Re: Print to agar vs seringe to agar? [Re: jpack666]
    #19075649 - 11/02/13 03:21 PM (10 years, 2 months ago)

Quote:

jpack666 said:
Hey hacker... Do you need to do isolations/petri transfers to get plates like on your signature. Or do you sometimes get lucky and just use one print, innoc like 10 plates and they all turn out without contams?



It depends entirely on how clean the print is. This will be different everywhere. Relevant to below

Quote:

jpack666 said:
(violet with her clean prints in vitro tek just got me thinking... How exactly unclean are prints usually? When you drop spores from the print on the agar, what are the probabilities of having contams and needing an isolation?)



I answered your question for you some in the culture tek thread.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


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InvisiblePastywhyteMDiscord
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Re: Print to agar vs seringe to agar? [Re: Violet]
    #19075767 - 11/02/13 03:51 PM (10 years, 2 months ago)

I have had prints from sponsors that were so contamed it was nearly impossible (not completely but close) to clean up. I have made prints myself that showed no contams on the initial inoculation. I would say that no print is 100% sterile (except maybe ones fruited invito as per Violet). This is why I never just fire some spores into a jar or grains or LC, its inoculate with agar or nothing at all.


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Invisiblejpack666
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Posts: 484
Re: Print to agar vs seringe to agar? [Re: Pastywhyte]
    #19093536 - 11/06/13 12:43 AM (10 years, 2 months ago)

Quote:

This is why I never just fire some spores into a jar or grains or LC, its inoculate with agar or nothing at all.




Thanks for reminding me, I was wondering if I could inoculate straw or dung with a seringe and you just might have made the best point: don't take chances because you don't know how clean the seringe is. better start from petri -- if aiming for ideal conditions.

Thanks for the comment


--------------------
"There's a negative and a positive to everything."  :mushroom2:

For more information, RogerRabbit's Website/Videos


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Invisiblejpack666
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Re: Print to agar vs seringe to agar? [Re: jpack666]
    #19157947 - 11/19/13 01:24 AM (10 years, 2 months ago)

This is stupid but I can't find a single video on how to do this...

Is there a prefered technique?

I mean, when you scrap off the spores with a sterile loop... They just "fall down on the agar"?
Do have to use the loop to make zoro style lines and such, or do you have to use the loop to melt the agar where the spores will fall?

Basically? How much am I over thinking this lol
Violet said 2-3 drops
Hacker said scrap a few with the ring
I remember reading somewhere someone saying use the zoro technique...

It's basic stuff, but some, like me, might wonder...

Thanks


--------------------
"There's a negative and a positive to everything."  :mushroom2:

For more information, RogerRabbit's Website/Videos


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Invisiblepoopy mcpooperson
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Registered: 10/17/13
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Re: Print to agar vs seringe to agar? [Re: jpack666]
    #19159306 - 11/19/13 11:46 AM (10 years, 2 months ago)

prints: take your heated loop and touch the agar, then touch your print, then touch your agar again. heat and repeat.

MS spores solution: one drop per section or agar plate is all you need.


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Invisiblejpack666
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Re: Print to agar vs seringe to agar? [Re: poopy mcpooperson]
    #19159385 - 11/19/13 12:15 PM (10 years, 2 months ago)

Thanks :-)


--------------------
"There's a negative and a positive to everything."  :mushroom2:

For more information, RogerRabbit's Website/Videos


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