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Anonymous #1

First time on agar no results!!!!
    #19069365 - 11/01/13 11:11 AM (10 years, 3 months ago)

First time on agar

1.i bought agar named potato dextrose agar
  Is it enough by it self to grow mycelium?
Or do I have to add something like
  Malt extract or yeasts.

2.i have inoculated 6 Petri dishes with spore
  A week ago. I see no growth in them.
  Isn't it supposed to be fast on agar, isn't it?
  Does it mean that the inoculation failed?

3.i also inoculated 3 micro boxes
  this time I cocked the agar inside the micro-box
  And inoculated with spore syringe.
  Again 5 days have pasted and I see nothing.

How long is it suppose to take to see significant growth on agar?
And how long should I Waite before declaring it failed.

Thanks in advance for your answers


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Anonymous #2

Re: First time on agar no results!!!! [Re: Anonymous #1]
    #19069519 - 11/01/13 11:49 AM (10 years, 3 months ago)

I never had luck with spores to agar.  Ever.

So much fail that I quit trying to grow.


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Anonymous #3

Re: First time on agar no results!!!! [Re: Anonymous #2]
    #19070028 - 11/01/13 01:54 PM (10 years, 3 months ago)

Why didn't you post this in the Cultivation forum?


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Anonymous #4

Re: First time on agar no results!!!! [Re: Anonymous #3]
    #19071688 - 11/01/13 07:21 PM (10 years, 3 months ago)

did you soak the spores in sterilized water first?


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Anonymous #2

Re: First time on agar no results!!!! [Re: Anonymous #4]
    #19072498 - 11/01/13 10:06 PM (10 years, 3 months ago)

Quote:

Anonymous #4 said:
did you soak the spores in sterilized water first?




He said it was a syringe...


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Anonymous #1

Re: First time on agar no results!!!! [Re: Anonymous #2]
    #19074375 - 11/02/13 10:06 AM (10 years, 3 months ago)

Why didn't you post this in the Cultivation forum?

it took me about a week to find how to post at all.
so the first place that i find that i could post in,
i jumped on the opportunity.
only then i realize my mistake.
sorry for any inconvenience.

did you soak the spores in sterilized water first?

i did spores with no soaking on the petri dishes
and i did spores that were socked in the micro box.

the petri dishes were inoculated tow days before the micro box
new 8 Day for the petri.
6 days on the micro box.

still no visible result.

i thought that the hole idea on petri and agar was
that you can see result very fast.

my lest grow took 3 month and is still on going.
4 cakes birthed,1 about a month ago,gave
Mushrooms,the second 2-3 weeks ago still nothing.
the last two 1 week ago this time with no dunk and rool
just dunk.


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Anonymous #4

Re: First time on agar no results!!!! [Re: Anonymous #1]
    #19074726 - 11/02/13 11:37 AM (10 years, 3 months ago)

i dont know about microboxes but spores on agar need soaking:
When first produced, spores are moist, inflated cells with a relatively high rate of germination.
As time passes, they dry, collapse at their centers and can not easily germinate. The probability of
germinating dehydrated spores increases by soaking them in sterilized water. For 30 minutes at 15
psi, sterilize an eye dropper or similar device (syringe or pipette) and a water filled test tube or
25-250 ml. Erlenmeyer flask stopped with cotton and covered with aluminum foil. Carefully touch
~ spores onto a scalpel and insert into sterile water. Tightly seal and let stand for 6-12 hours.


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