Home | Community | Message Board


This site includes paid links. Please support our sponsors.


Welcome to the Shroomery Message Board! You are experiencing a small sample of what the site has to offer. Please login or register to post messages and view our exclusive members-only content. You'll gain access to additional forums, file attachments, board customizations, encrypted private messages, and much more!

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder

Jump to first unread post Pages: 1
Some of these posts are very old and might contain outdated information. You may wish to search for newer posts instead.
Offlineflipsidetrue
Male User Gallery


Folding@home Statistics
Registered: 05/26/13
Posts: 364
Loc: Earth
Last seen: 7 years, 10 months
Thinking about using short half pint jars instead of petri dishes
    #19071526 - 11/01/13 06:49 PM (10 years, 2 months ago)

So i got some Anti biotic MEA and a sleeve of 20 pre sterilized petri dishes about a week ago, instead of using all 20 i only used 6 i thought i was being safe by doing so in case anything went wrong and i didnt wanna waste the agar but now i think i made a mistake by not doing because now i have an opened bag of 14 petri dishes and i doubt there sterile now.

Well after inoculating 3 dishes with a spore syringe and adding tissue to clone from fruit to 3 dishes I've had the same result with both,
All 6 have gotten cobweb mold in them, so im thinking of just using short wide mouthed half pint jars instead to insure a more sterile procedure.


--------------------
DOWNLOAD LETS GROW MUSHROOMS


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Thinking about using short half pint jars instead of petri dishes [Re: flipsidetrue]
    #19071603 - 11/01/13 07:07 PM (10 years, 2 months ago)

no-pour agar tek


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
OfflineStromriderM
This must be the place
Male User Gallery


Registered: 06/02/13
Posts: 7,326
Loc: Dept of know what I'm say... Flag
Last seen: 37 minutes, 51 seconds
Trusted Cultivator
Re: Thinking about using short half pint jars instead of petri dishes [Re: SpitballJedi]
    #19071633 - 11/01/13 07:12 PM (10 years, 2 months ago)

I've tried both. I definitely prefer the pre-sterilized plastic dishes. I always make all 20 of them and then package them 2 to a zip lock bag. I stick them in the top of my closest and use them as needed


Extras: Filter Print Post Top
Offlineflipsidetrue
Male User Gallery


Folding@home Statistics
Registered: 05/26/13
Posts: 364
Loc: Earth
Last seen: 7 years, 10 months
Re: Thinking about using short half pint jars instead of petri dishes [Re: Stromrider]
    #19072454 - 11/01/13 09:55 PM (10 years, 2 months ago)

yea i wish i would have done that  :facepalm3:  i think I'll just go ahead and do the rest after i give my SAB a cleaning since i seem to successfully grow cobweb I'm planning on using the rest of my 14 dishes i have left since i didn't use them but should i worry about those being con tamed?  :wizardfail:


--------------------
DOWNLOAD LETS GROW MUSHROOMS


Edited by flipsidetrue (11/01/13 09:57 PM)


Extras: Filter Print Post Top
InvisibleSpitballJedi
Ancient Astronaut
Male User Gallery


Registered: 10/13/12
Posts: 8,598
Loc: Nibiru Flag
Trusted Cultivator
Re: Thinking about using short half pint jars instead of petri dishes [Re: flipsidetrue]
    #19073967 - 11/02/13 08:03 AM (10 years, 2 months ago)

If they've been sitting in open air, I would be concerned about contamination.

Like I mentioned on your other thread, If you are getting the same contamination with both spores and tissue, I would suspect your technique is at fault.

I can't explain why your poured blank petris are not contaminated after sitting in your closet, unwrapped, in the open, without contaminating, but then show cobweb soon after inoculating.

I suspect it's just a time delay.

In your other thread, I suspected the tape you were using to wrap the inoculated plates was the problem. That was the basis of my questions.

But, you stumped me when you said the poured UN-inoculated (blank) plates were not protected at all.


--------------------
The Basics
A little civility goes a long way

The Noob Forum
The Hammock Hangers' Forum


Extras: Filter Print Post Top
Offlinerumfor69
Bodhicitta Cultivator
Male


Registered: 08/05/11
Posts: 6,767
Loc: In the Gills
Last seen: 15 hours, 1 minute
Re: Thinking about using short half pint jars instead of petri dishes [Re: SpitballJedi]
    #19074121 - 11/02/13 08:53 AM (10 years, 2 months ago)

I use the short wide mouth jars for petri's with an sfd on them. 

Only bad part, you cant look down from the top unless its open.

You can see up through the bottom though. I just mix enough PDA right 

in each jar to cover the bottom then PC.


--------------------

Links For Beginners And Beyond


Extras: Filter Print Post Top
InvisibleViolet
 User Gallery


Registered: 12/06/11
Posts: 4,205
Re: Thinking about using short half pint jars instead of petri dishes [Re: rumfor69]
    #19074163 - 11/02/13 09:10 AM (10 years, 2 months ago)

Container agar dishes, saved my life.


--------------------
Intentionally or not, here in mushcult we are purveyors of love culture and enlightenment movement. Let's try to act like it!

PODS TEK - Growing Invitro with BRF/verm or Grass Seed containers
The simplest, quickest, safest tek!  For beginners, culturers, lazy people, stealth lovers, contam haters, and alternative seekers!
Violet's Teks and Posts


Extras: Filter Print Post Top
Offlineflipsidetrue
Male User Gallery


Folding@home Statistics
Registered: 05/26/13
Posts: 364
Loc: Earth
Last seen: 7 years, 10 months
Re: Thinking about using short half pint jars instead of petri dishes [Re: SpitballJedi]
    #19075270 - 11/02/13 01:57 PM (10 years, 2 months ago)

well i opened the sleeve in the SAB and closed the bag back and put them in the closet also, I'm gunna pour the rest tonight and place in bags until i use them I'll prob wait a week and see if contams grow in them before i add anything else to them, and the ones i stored in the closet without wrapping then or putting them in a bag sat there in the closet for about 3 to 4 day sand i would imagine contams would have taken over by then but maybe i didnt wait long enough? and then when i added spores or tissue it was just a matter of time like you said.

BTW I greatly appreciate all your words and advice you've chipped in its helped me out a whole bunch!


--------------------
DOWNLOAD LETS GROW MUSHROOMS


Extras: Filter Print Post Top
Jump to top Pages: 1

Shop: Mushroom-Hut Liquid Cultures   Original Sensible Seeds Bulk Cannabis Seeds   Left Coast Kratom Buy Kratom Extract   North Spore Cultivation Supplies   PhytoExtractum Maeng Da Thai Kratom Leaf Powder


Similar ThreadsPosterViewsRepliesLast post
* Rye in half pint jars MycoGlowFlow 2,543 2 02/04/02 09:32 AM
by bluhoney
* Half-Pint Jars alen 1,642 3 09/10/01 08:23 PM
by Dead Shaman
* half pint jars Dane 1,313 11 02/18/02 06:56 PM
by Anno
* Re: Need help finding shoulderless half pint jars magnusra 945 2 04/29/01 08:39 AM
by MNmyc
* Re: Tricks for pint jars? psilocyberV 1,149 7 06/10/00 01:48 AM
by 3Mshroom
* One Pint Jar Progress Nomez 2,826 12 02/15/20 08:58 PM
by MaddoxA
* Half-Assed Half-Pint windex 1,819 10 09/14/01 05:33 PM
by ShroomieWv
* Pint jars ok? or definitely not? Absolut 3,295 17 02/15/20 09:48 PM
by CatsLoveHouseMusic

Extra information
You cannot start new topics / You cannot reply to topics
HTML is disabled / BBCode is enabled
Moderator: Shroomism, george castanza, RogerRabbit, veggie, mushboy, fahtster, LogicaL Chaos, 13shrooms, Stipe-n Cap, Pastywhyte, bodhisatta, Tormato, Land Trout, A.k.a
887 topic views. 45 members, 251 guests and 34 web crawlers are browsing this forum.
[ Show Images Only | Sort by Score | Print Topic ]
Search this thread:

Copyright 1997-2024 Mind Media. Some rights reserved.

Generated in 0.026 seconds spending 0.008 seconds on 14 queries.